超声介导的p选择素靶向和大麻素2型受体激动剂微泡破坏改善大鼠骨骼肌缺血-再灌注损伤

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2023-10-01 DOI:10.1166/jbn.2023.3560
Lei Zhang, Qi Yang, Wei Wu, Huiyang Wang, Zhichao Ding, Hebin Zhang, Laizhi Zhang, Hao Wu
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摘要

本研究旨在探讨超声联合p选择素靶向/载AM1241微泡(AM1241 mb)对骨骼肌缺血再灌注(I/R)损伤大鼠的改善作用。采用旋转蒸发和机械震动法制备AM1241 MB。H&E染色检测骨骼肌组织学病变,TUNEL染色检测骨骼肌细胞凋亡。ELISA法分析小鼠骨骼肌组织抗氧化能力和炎症反应的变化。western blot检测Nfr2/HO-1通路相关蛋白的变化。由此可见,超声介导的AM1241 mb破坏可显著改善I/R损伤大鼠骨骼肌组织的损伤程度和水肿程度。骨骼肌组织的炎症反应和细胞凋亡也明显改善,抗氧化能力明显增强。Western blot分析显示,超声联合AM1241 mb处理激活了Nrf2/HO-1通路。然而,选择性敲低Nrf2显著逆转了超声联合AM1241 mb治疗骨骼肌I/R损伤的有益作用。超声介导破坏AM1241 mb可通过激活Nrf2/HO-1通路,有效改善大鼠骨骼肌组织I/R损伤。
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Ultrasound-Mediated Destruction of P-Selectin-Targeted and Cannabinoid Type 2 Receptor Agonist-Loading Microbubbles Ameliorates Skeletal Muscle Ischemia-Reperfusion Injury in Rat
Current study aims to explore the ameliorative effects of ultrasound in combination with P-selectin-targeted/AM1241-loaded microbubbles (AM1241 MBs) in rats with skeletal muscle ischemia/reperfusion (I/R) injury. AM1241 MB was prepared via rotary evaporation and mechanical shaking. Histological lesions and cellular apoptosis in skeletal muscle were detected by H&E staining and TUNEL staining, respectively. Changes in antioxidant capacity and inflammatory response in skeletal muscle tissue were analyzed by ELISA method. Changes of Nfr2/HO-1 pathway-related proteins were measured via the western blot. As a result, ultrasound-mediated destruction of AM1241 MBs significantly improved the degree of damage and edema in skeletal muscle tissue of rats with I/R injury. Inflammatory response and cellular apoptosis in skeletal muscle tissue were also significantly improved, while the antioxidant capacity was significantly enhanced. Western blot analysis exhibited that ultrasound in combination with AM1241 MBs treatment activated the Nrf2/HO-1 pathway. Nevertheless, selective knockdown of Nrf2 significantly reversed the beneficial effects of ultrasound combined with AM1241 MBs treatment on I/R injuries of the skeletal muscle. Ultrasound-mediated disruption of AM1241 MBs could effectively ameliorate the I/R injuries of skeletal muscle tissues in rats via activating the Nrf2/HO-1 pathway.
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CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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