饲粮添加脂肪酸和矿质元素来源后,公牛组织中ω-3和ω-6家族必需多不饱和脂肪酸积累的特异性

IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Agricultural Science and Practice Pub Date : 2023-11-01 DOI:10.15407/agrisp10.02.062
O. B. Diachenko, J. F. Rivis, G. V. Tesak, O. I. Stadnytska
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Copper content was determined by the method of atomic absorption spectro- photometry using the Selmi C-115 M1 device. Results. It was found that the introduction of linseed oil (as a source of α-linolenic acid, which is a precursor of polyunsaturated fatty acids of the ω-3 family) and sunflower oil (as a source of linoleic acid, which is a precursor of polyunsaturated fatty acids of the ω-6 family), the synthetic substance doxane (as an inhibitor of biohydrogenation processes in unsaturated fatty acids in the rumen) and pentahydrate copper sulfate (as a source of copper) to the diet of young fattening cattle caused a probable increase in the content of biologically active polyunsaturated fatty acids of the ω-3 and ω-6 family and copper in their liver and skeletal muscles. 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引用次数: 0

摘要

的目标。测定在饲粮中添加必需脂肪酸和硫酸铜源后,肥育公牛的生长强度和ω-3和ω-6家族必需多不饱和脂肪酸在其组织中积累的特异性,以提高其产肉率,提高牛肉的生物学价值。方法。本研究采用国际通用的方法学方法,按照ISO 17025的要求进行,并采用涉及临床健康动物的同行组的常规方法进行。采用Chrom-5气相色谱法测定了ω-3和ω-6族多不饱和脂肪酸的含量。采用Selmi C-115 M1原子吸收分光光度法测定铜的含量。结果。研究发现,引入亚麻籽油(α-亚麻酸的来源,它是ω-3族多不饱和脂肪酸的前体)和葵花籽油(亚油酸的来源,它是ω-6族多不饱和脂肪酸的前体),在育肥幼牛日粮中添加合成物质十二烷(作为瘤胃不饱和脂肪酸生物氢化过程的抑制剂)和五水硫酸铜(作为铜的来源),可能会增加其肝脏和骨骼肌中具有生物活性的ω-3和ω-6家族多不饱和脂肪酸和铜的含量。同时,由于体内代谢过程的刺激,上述组织中ω-3和ω-6家族的生物活性多不饱和脂肪酸和铜的含量增加,可能是育肥幼畜平均日增重增加的原因。由此可见,饲粮中α-亚麻酸、亚油酸和铜的含量与实验动物组织中α-亚麻酸、亚油酸和铜的含量、生产特性和牛肉的生物学价值存在直接关系。结论。在育肥公牛日粮中添加亚麻籽油和葵花籽油的混合物,导致α-亚麻酸和亚油酸含量增加,ω-6族必需多不饱和脂肪酸与ω-3族必需多不饱和脂肪酸的比值降低1.7倍。增肥公牛日粮中铜和ω-6和ω-3族必需多不饱和脂肪酸含量的增加,导致其在肝脏和骨骼肌中积累,从而提高了牛肉的生物学价值。
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Specificities of accumulation of essential polyunsaturated fatty acids of ω-3 and ω-6 families in the tissues of bulls after supplementing their diet with the sources of fatty acids and mineral elements
Aim. To determine the intensity of growth of fattening bulls and the specificities of the accumulation of essential polyunsaturated fatty acids from the ω-3 and ω-6 families in their tissues after supplementing their diet with the sources of essential fatty acids and copper sulfate to increase their meat productivity and improve the biological value of beef. Methods. The study was conducted using the methodological approaches, common for international practice in accordance with the requirements of ISO 17025, and using the conventional methods of peer groups involving clinically healthy animals. The content of polyunsaturated fatty acids of the ω-3 and ω-6 families was determined by gas chro- matography using the Chrom-5 device. Copper content was determined by the method of atomic absorption spectro- photometry using the Selmi C-115 M1 device. Results. It was found that the introduction of linseed oil (as a source of α-linolenic acid, which is a precursor of polyunsaturated fatty acids of the ω-3 family) and sunflower oil (as a source of linoleic acid, which is a precursor of polyunsaturated fatty acids of the ω-6 family), the synthetic substance doxane (as an inhibitor of biohydrogenation processes in unsaturated fatty acids in the rumen) and pentahydrate copper sulfate (as a source of copper) to the diet of young fattening cattle caused a probable increase in the content of biologically active polyunsaturated fatty acids of the ω-3 and ω-6 family and copper in their liver and skeletal muscles. At the same time, the increase in the content of biologically active polyunsaturated fatty acids of the ω-3 and ω-6 families and copper in the abovementioned tissues due to the stimulation of metabolic processes in the body contributed to a probable increase in the average daily weight gain of young fattening animals. Thus, there was a direct relationship between the content of α-linolenic and linoleic acids and copper in the diet and their content in the tissues of experimental animals, productivity characteristics, and biological value of beef. Conclusions. The introduction of a mixture of linseed and sunflower oils into the diet of fattening bulls led to an increase in the content of α-linolenic and linoleic acids and a 1.7-fold decrease in the ratio between essential polyunsaturated fatty acids of the ω-6 family and that of the ω-3 family. The increase in the content of copper and essential polyunsaturated fatty acids of the ω-6 and ω-3 families in the diet of fattening bulls led to their accumulation in the liver and skeletal muscles, which contributed to the enhanced biological value of beef.
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Agricultural Science and Practice
Agricultural Science and Practice AGRICULTURE, MULTIDISCIPLINARY-
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