两种地方食用菌(侧耳菌属)的诊断及其生产管理

Q4 Medicine Revista Bionatura Pub Date : 2023-10-15 DOI:10.21931/rb/css/2023.08.03.56
Abeer Kadhim Jasim Al-Falahi, Ahmed Kareem Alsadaawy, Rukaibaa Ali Chechan
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引用次数: 0

摘要

本研究利用内部转录间隔序列(ITS)证实了两种伊拉克蘑菇的身份;利用ITS1和ITS4引物扩增两株分离菌株的基因组DNA,并利用NCBI数据库进行BLAST (Basic Local Alignment Tool)检索。结果表明,两种蘑菇的核苷酸序列与基因库中的平菇(Pleurotus ostreatus)和平菇(Pleurotus eryngii)序列的匹配度分别为99%和100%。本研究的第二阶段是比较不同农业废弃物,包括麦秸(WS)、玉米芯(CC)和锯末(SD)添加25%麦麸(W)和2%碳酸钙(CaCo3)对当地平菇菌丝生长、产量和生物效率(BE)的影响,并与中国菌种平菇(pleurotus ostreatus121)和平菇(pleurotus eryngii 080)进行比较。在SD基质上,局部大叶假单胞菌的平均生长最高为1.50 cm,而大叶假单胞菌的平均生长最高为1.18 cm。在相同的基质条件下,CC最适合蘑菇本地产白顶菇和本地产白顶菇的产量分别为485.40和418.50 g/袋,而本地产白顶菇的产量为470.40 g/袋。同时发现WS底物对各本地分离株和中国菌株的BE均最高。关键词:伊拉克菌株,侧耳菌,农业废弃物,野生农用蘑菇
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Diagnosis of two local mushroom species (Pleurotus spp.) and their production management
The present study confirmed the identity of two Iraqi mushrooms by using the internal transcribed spacer (ITS) sequence; the genomic DNA of two isolates was amplified using ITS1 and ITS4 primers, and the data analyzed through Basic Local Alignment Tool( BLAST) search was achieved using the National Center for Biotechnology Information (NCBI) database. The result shows that the nucleotide sequence of two mushrooms blasted against the sequence from the Gene Bank database that Pleurotus ostreatus and Pleurotus eryngii matched 99% and 100%, respectively. The second stage of the current study was the comparison of different agro-waste, including wheat straw(WS), corncob(CC) and sawdust(SD) supplemented with 25% wheat bran(W) and 2% calcium carbonate (CaCo3) on mycelium growth, yield and biological efficiency (BE) of local oyster mushroom which comparison with Chinese strain pleurotus ostreatus121 and pleurotus eryngii 080. The highest growth average of local P. ostretus on the SD substrate was 1.50 cm, and P.eryngii was recorded at 1.18 cm. The substrate CC was the most suitable for the yield of mushroom local P. ostreatus and P. ostreatus121(485.40 and 418.50 g/bag, respectively), while local P. eryngii recorded 470.40 in the same substrate. It was also found that the WS substrate recorded the highest BE for each local isolate and Chinese strain. Keywords: Iraqi strains, Pleurotus spp., agricultural wastes, wild agricultural mushrooms
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来源期刊
Revista Bionatura
Revista Bionatura Medicine-Epidemiology
CiteScore
0.50
自引率
0.00%
发文量
144
审稿时长
8 weeks
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