edta血新鲜度对地中海贫血筛查试验可靠性的影响

IF 0.2 Q4 MULTIDISCIPLINARY SCIENCES Suranaree Journal of Science and Technology Pub Date : 2023-05-29 DOI:10.55766/sujst-2023-02-e02352
Patiwat Kongdang, Thanusak Tatu
{"title":"edta血新鲜度对地中海贫血筛查试验可靠性的影响","authors":"Patiwat Kongdang, Thanusak Tatu","doi":"10.55766/sujst-2023-02-e02352","DOIUrl":null,"url":null,"abstract":"Thalassemia screening tests include one tube (0.36% NaCl) osmotic fragility test (OFT), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). Reliability of these parameters in stored EDTA blood is the important issue to be concerned. To address this issue, we analyzed the results of these thalassemia screening tests in 50 fresh and stored EDTA blood samples of collected in the Chiang Mai province of northern Thailand. These blood samples; 25 with MCV < 80 fl and 25 with MCV ≥ 80 fl, were stored at room temperature (RT) (approximately 30°C) and in cold (approximately 4°C). OFT, MCV, and MCH were tested in both groups of blood samples everyday for 7 days. It was found that falsely negative OFT results were seen after 1 day and 4 days of storage at RT and in cold, respectively. For MCV, acceptable values were obtained at 0 day and 2-3 days storage at RT and in cold, respectively. No change of MCH values was observed throughout 7-day storage at both RT and cold. It was concluded that when performing thalassemia screening tests in Thailand, fresh EDTA blood must be used for OFT test and MCV determination. However, although MCH seemed to be the most stable parameters, thalassemia screen cannot rely only on MCH value. Therefore, thalassemia screen using these three tests must be done within blood collection day if no cooling facility is available. This information would also be useful for countries in tropical region where ambient temperature is high.","PeriodicalId":43478,"journal":{"name":"Suranaree Journal of Science and Technology","volume":"9 1","pages":"0"},"PeriodicalIF":0.2000,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"INFLUENCE OF FRESHNESS OF EDTA BLOOD ON RELIABILITY OF THALASSEMIA SCREENING TESTS\",\"authors\":\"Patiwat Kongdang, Thanusak Tatu\",\"doi\":\"10.55766/sujst-2023-02-e02352\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Thalassemia screening tests include one tube (0.36% NaCl) osmotic fragility test (OFT), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). Reliability of these parameters in stored EDTA blood is the important issue to be concerned. To address this issue, we analyzed the results of these thalassemia screening tests in 50 fresh and stored EDTA blood samples of collected in the Chiang Mai province of northern Thailand. These blood samples; 25 with MCV < 80 fl and 25 with MCV ≥ 80 fl, were stored at room temperature (RT) (approximately 30°C) and in cold (approximately 4°C). OFT, MCV, and MCH were tested in both groups of blood samples everyday for 7 days. It was found that falsely negative OFT results were seen after 1 day and 4 days of storage at RT and in cold, respectively. For MCV, acceptable values were obtained at 0 day and 2-3 days storage at RT and in cold, respectively. No change of MCH values was observed throughout 7-day storage at both RT and cold. It was concluded that when performing thalassemia screening tests in Thailand, fresh EDTA blood must be used for OFT test and MCV determination. However, although MCH seemed to be the most stable parameters, thalassemia screen cannot rely only on MCH value. Therefore, thalassemia screen using these three tests must be done within blood collection day if no cooling facility is available. This information would also be useful for countries in tropical region where ambient temperature is high.\",\"PeriodicalId\":43478,\"journal\":{\"name\":\"Suranaree Journal of Science and Technology\",\"volume\":\"9 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2023-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Suranaree Journal of Science and Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55766/sujst-2023-02-e02352\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Suranaree Journal of Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55766/sujst-2023-02-e02352","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

地中海贫血筛查试验包括1管(0.36% NaCl)渗透脆性试验(OFT)、平均红细胞体积(MCV)、平均红细胞血红蛋白(MCH)。储存EDTA血液中这些参数的可靠性是值得关注的重要问题。为了解决这一问题,我们分析了在泰国北部清迈省收集的50份新鲜和储存的EDTA血液样本的地中海贫血筛查试验结果。这些血液样本;25与MCV <80 fl和MCV≥80 fl的25份分别在室温(约30°C)和低温(约4°C)下保存。每天检测两组血液样本的OFT、MCV和MCH,连续7天。结果发现,在室温和低温条件下分别保存1天和4天后,OFT结果为假阴性。对于MCV,分别在室温和低温下储存0天和2-3天获得可接受值。在室温和低温条件下,MCH值在7天的贮藏过程中没有变化。因此,在泰国进行地中海贫血筛查试验时,必须使用新鲜EDTA血液进行OFT试验和MCV测定。然而,虽然MCH似乎是最稳定的参数,但地中海贫血筛查不能仅依赖MCH值。因此,如果没有冷却设备,必须在采血当天使用这三种检查进行地中海贫血筛查。这一信息对环境温度较高的热带地区国家也很有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
INFLUENCE OF FRESHNESS OF EDTA BLOOD ON RELIABILITY OF THALASSEMIA SCREENING TESTS
Thalassemia screening tests include one tube (0.36% NaCl) osmotic fragility test (OFT), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). Reliability of these parameters in stored EDTA blood is the important issue to be concerned. To address this issue, we analyzed the results of these thalassemia screening tests in 50 fresh and stored EDTA blood samples of collected in the Chiang Mai province of northern Thailand. These blood samples; 25 with MCV < 80 fl and 25 with MCV ≥ 80 fl, were stored at room temperature (RT) (approximately 30°C) and in cold (approximately 4°C). OFT, MCV, and MCH were tested in both groups of blood samples everyday for 7 days. It was found that falsely negative OFT results were seen after 1 day and 4 days of storage at RT and in cold, respectively. For MCV, acceptable values were obtained at 0 day and 2-3 days storage at RT and in cold, respectively. No change of MCH values was observed throughout 7-day storage at both RT and cold. It was concluded that when performing thalassemia screening tests in Thailand, fresh EDTA blood must be used for OFT test and MCV determination. However, although MCH seemed to be the most stable parameters, thalassemia screen cannot rely only on MCH value. Therefore, thalassemia screen using these three tests must be done within blood collection day if no cooling facility is available. This information would also be useful for countries in tropical region where ambient temperature is high.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Suranaree Journal of Science and Technology
Suranaree Journal of Science and Technology MULTIDISCIPLINARY SCIENCES-
CiteScore
0.30
自引率
50.00%
发文量
0
期刊最新文献
REDUCTION IN GREENHOUSE GAS EMISSIONS FROM COCONUT MILK PRODUCTION PLANTS IN THAILAND ADSORPTION OF DIBENZOXAZEPINE GAS ON TRANSITION METAL-DOPED SILICON CARBIDE NANOTUBES: A THEORETICAL INVESTIGATION PRELIMINARY DEVELOPMENT OF NURSES’ PRACTICE OF PEACEFUL END-OF-LIFE CARE INSTRUMENT (NP-PECI) PREVALENCE OF SICKLE CELL ANEMIA IN YOUTH BY COST EFFECTIVE STRATEGY ALONG WITH HPLC ADAPTIVE TRAFFIC SYSTEM CONTROLLERS IN TRAFFIC ENGINEERING : A SURVEY
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1