抑癌基因<i>APC</i> <i>GSTP1</i> <i>RASSF1A</i>前列腺特异性抗原相关标志物含量在前列腺癌诊断中的意义

Q4 Biochemistry, Genetics and Molecular Biology Sibirskii nauchnyi meditsinskii zhurnal Pub Date : 2023-10-31 DOI:10.18699/ssmj20230518
I. A. Aboyan, E. N. Fedotova, A. Yu. Maksimov, E. F. Кomarova, S. N. Vlasov
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引用次数: 0

摘要

尽管前列腺癌(PCa)诊断方法的改进和前列腺特异性抗原(PSA)监测的引入,晚期前列腺癌在俄罗斯的发病率仍然很高。早期和高级别前列腺癌的有效诊断需要新的更多信息变量。在表观遗传学领域,特别感兴趣的是DNA超甲基化的检测,它可以作为PCa的生物标志物,因为它很容易发现并可能诱导稳定的适当基因沉默,导致显著的细胞变化。本研究的目的是分析APC、GSTP1和RASSF1A基因甲基化与前列腺癌总PSA和前列腺健康指数(PHI)的关系。材料和方法。本研究包括54例疑似PCa的患者,年龄在75岁以下,初始总PSA水平为2.5至10 ng/ml。采用化学发光免疫法测定血清中总PSA、游离PSA和早成熟PSA ([-2] prosa)的浓度,计算PHI值。结果和讨论。该研究揭示了血浆样本中甲基化程度与活检材料中仅GSTP1基因和psa相关标记物之间具有统计学意义的相关性。我们发现参数的偶然性随着Gleason评分的增加而增加。结论。这项工作的结果是,在GSTP1基因启动子区域甲基化模式的变化和PHI之间发现了统计上显著的直接相关性,这使我们能够将它们作为潜在的候选者纳入诊断小组,以更有效地早期检测前列腺癌。
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The relationship between methylation of tumor suppressor genes <i>APC</i>, <i>GSTP1</i>, <i>RASSF1A</i> and content of prostate-specific antigen- associated markers in prostate cancer diagnosis
Despite the improvement of prostate cancer (PCa) diagnostic methods and the introduction of prostate-specific antigen (PSA) monitoring, the incidence of advanced PCa in Russia remains high. New more informative variables are needed for the effective diagnosis of early stage and high-grade PCa. In the field of epigenetics of special interest is the detection of DNA hypermethylation, which can serve as a PCa biomarker, since it is common to find and may induce a stable appropriate gene silencing, leading to significant cell changes. Aim of the study was to analyze the relationship between methylation of the APC, GSTP1 and RASSF1A genes and total PSA and prostate health index (PHI) in PCa. Material and methods. The present study included 54 patients with suspicion of PCa, up to 75-years old, who had an initial total PSA level from 2.5 to 10 ng/ml. To calculate PHI value the concentrations of total PSA, free PSA and pre-mature form of PSA ([–2]proPSA) in blood serum were measured by chemiluminescence immunoassay. Results and discussion. The study has revealed the statistically significant correlation between the degree of methylation in blood plasma samples and biopsy material of only the GSTP1 gene and PSA-associated markers. We found an increase of parameters contingency as Gleason score increased. Conclusions. As a result of this work, statistically significant direct correlations were identified between changes in methylation patterns of the promoter region of the GSTP1 gene and PHI, which allows us to consider them as potential candidates for inclusion in a diagnostic panel for more effective early detection of prostate cancer.
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来源期刊
CiteScore
0.40
自引率
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发文量
54
审稿时长
12 weeks
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