从中国宁夏分离的人类埃可病毒 30 的遗传特征和分子流行病学研究

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Biosafety and Health Pub Date : 2023-12-01 DOI:10.1016/j.bsheal.2023.10.001
Fang Yuan , Xinfeng Wei , Xueping Ma , Jiangtao Ma , Xuemin Ma , Xiaoqiang Sun , Min Cao , Juan Zhou , Wei Zhang , Hui Chen , Rui Wang , Jichen Li , Qiang Sun
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引用次数: 0

摘要

2017年10月,宁夏回族自治区一所小学发生了9例与无菌性脑膜炎相关的埃可病毒30(E30)小规模暴发疫情。同年,我们在急性弛缓性麻痹(AFP)病例监测系统中观察到 E30 的水平显著上升。为了研究它们之间的系统发育关系,我们测定了从无菌性脑膜炎病例、急性弛缓性麻痹病例和健康儿童中分离出的 12 株菌株的全基因组序列。我们发现,在宁夏流行的E30菌株属于两个系(H系和J系)。2010年、2012年和2016年分离到的菌株属于H系。2017 年,一个新的品系 J 出现,成为优势品系。根据全基因组和P1、P2和P3区构建了系统发生树;发现了与其他类型肠道病毒B种的聚类,表明发生了重组事件。重组位点主要分布在 2B、2C 和 3D 区。这项研究证实,2010年、2012年和2016年宁夏的E30毒株具有不同的重组模式,并与不同的肠道病毒重组。2017年流行的E30来源于另一个新品系,具有复杂的重组模式,并在宁夏形成了独立的传播链。
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Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China

In October 2017, a small outbreak of echovirus 30 (E30) associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region. That year, we observed a significant increase in E30 levels in an acute flaccid paralysis (AFP) case surveillance system. To investigate their phylogenetic relationships, we determined the whole genomic sequences of 12 strains isolated from aseptic meningitis cases, AFP cases, and healthy children. We found that the E30 strains circulating in Ningxia belong to two lineages (H and J). The strains isolated in 2010, 2012, and 2016 belonged to the H lineage. In 2017, a new lineage, J, emerged as the dominant lineage. Phylogenetic trees were constructed based on the whole genome and P1, P2, and P3 regions; clustering with other types of enterovirus species B was found, suggesting that recombination events had occurred. The recombination sites were mainly in regions 2B, 2C, and 3D. This study confirmed that the E30 strains in Ningxia in 2010, 2012, and 2016 had different recombination patterns and were recombined with different enteroviruses. The 2017 epidemic E30 originated from another new lineage with a complex recombination pattern and formed an independent transmission chain in Ningxia.

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来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
期刊最新文献
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