芒果叶生物活性成分中芒果苷的含量测定及创面愈合电位评价

Q3 Pharmacology, Toxicology and Pharmaceutics Jordan Journal of Pharmaceutical Sciences Pub Date : 2023-09-23 DOI:10.35516/jjps.v16i3.652
Valdy Filando Sardi, Astika Astika, Ilza Milenia Jalius, Friardi Ismed
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引用次数: 0

摘要

烧伤是指由于暴露在高温下而对皮肤表面造成的损伤,这可能是由于油、水、电、火、阳光照射和化学物质等因素造成的。及时和适当的治疗对于防止不良后果至关重要。因此,本研究旨在量化芒果叶(Mangifera indica L.)生物活性成分中的芒果苷,这是一种潜在的烧伤治疗方法,并评估其在烧伤愈合中的有效性。所采用的方法包括薄层色谱(TLC)-密度法,并进行了验证措施,包括线性度、检测和定量限(LoD和LoQ)、精密度、准确度和定量。在膜中配制浓度为5%、10%和15%的生物活性组分。将这些膜应用于先前遭受6次创面烧伤的家兔,并通过每3天使用游标卡尺测量烧伤直径来监测愈合进展,共21天。活性化合物芒果苷的检测波长为257 nm。结果表明,线性方程为y = 76496x + 2935.7,相关系数为0.9957,检出限为2.01µg/mL,定量限为6.07µg/mL,变异系数为0.59% ~ 3.33%,准确度为99.18% ~ 100.9%,芒果苷含量为208.31µg/mL。对生物活性芒果苷部分的膜制剂进行了兔二度烧伤的评价,其中浓度为10%和15%的效果最好。
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Quantification of Mangiferin from the Bioactive Fraction of Mango Leaves (Mangifera indica L.) and Evaluation of Wound-Healing Potential
Burns refer to damage to the skin's surface caused by exposure to high temperatures, which can be due to factors such as oil, water, electricity, fire, sun exposure, and chemicals. Prompt and appropriate treatment is essential to prevent undesirable consequences. Thus, this study aimed to quantify mangiferin, a potential treatment for burns, in the bioactive fraction of mango leaves (Mangifera indica L.) and evaluate its effectiveness in healing burns.The methods employed included thin-layer chromatography (TLC)-densitometry with validation measures, including linearity, detection and quantification limits (LoD and LoQ), precision, accuracy, and quantification. The bioactive fraction was formulated in membranes at concentrations of 5%, 10%, and 15%. These membranes were applied to rabbits previously subjected to six wound burns, and the healing progress was monitored by measuring burn diameter using a vernier caliper every 3 days for a total of 21 days. Mangiferin, the active compound, was detected at a wavelength of 257 nm. Test results yielded a linearity equation, y = 76496x + 2935.7, with a correlation coefficient value of 0.9957, a detection limit of 2.01 µg/mL, a quantification limit of 6.07 µg/mL, a coefficient of variation ranging from 0.59% to 3.33%, and an accuracy range of 99.18% to 100.9%, with mangiferin levels at 208.31 µg/mL. The membrane preparations of the bioactive mangiferin fraction were evaluated on second-degree burns in rabbits, with concentrations of 10% and 15% showing the most effectiveness.
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来源期刊
Jordan Journal of Pharmaceutical Sciences
Jordan Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
1.70
自引率
0.00%
发文量
33
期刊介绍: The Jordan Journal of Pharmaceutical Sciences (JJPS) is a scientific, bi-annual, peer-reviewed publication that will focus on current topics of interest to the pharmaceutical community at large. Although the JJPS is intended to be of interest to pharmaceutical scientists, other healthy workers, and manufacturing processors will also find it most interesting and informative. Papers will cover basic pharmaceutical and applied research, scientific commentaries, as well as views, reviews. Topics on products will include manufacturing process, quality control, pharmaceutical engineering, pharmaceutical technology, and philosophies on all aspects of pharmaceutical sciences. The editorial advisory board would like to place an emphasis on new and innovative methods, technologies, and techniques for the pharmaceutical industry. The reader will find a broad range of important topics in this first issue.
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