俄罗斯达吉斯坦两个自然气候区(山地和平原)红草原和高加索褐牛品种产奶量相关基因多态性分析

A. A. Ozdemirov, A. A. Khozhokov, Z. M. Huseynova, M. A. Daveteeva
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引用次数: 0

摘要

的目标。达吉斯坦高加索地区独特的地方品种基因库受到日益广泛使用商业品种的威胁,这反过来又有可能缩小增加和维持现有遗传多样性所必需的遗传基础。因此,本研究旨在研究达吉斯坦条件下饲养的红草原牛和高加索褐牛PRL、PIT‐1、GH基因的多态性。方法。采用PCR - RFLP方法对红草原牛和高加索褐牛进行基因分型。利用这些方法进行了遗传分析,研究了催乳素、生长激素和垂体特异性转录因子基因的多态性。结果。对红草原牛试验组进行基因分型分析,发现红草原牛的2个等位基因和3个基因型(PIT‐1A和PIT‐1B;PIT‐1基因的PIT‐1AA, PIT‐1BB, PIT‐1AB)具有特异性。PRL基因等位基因谱的特异性表现为PRLA等位基因出现频率高(0.84),PRLB等位基因出现频率低(0.16)。这反映在同源和杂合基因型的存在上,发生频率为73.0;23.0;分别为4.0%。结论。应用遗传方法来提高牲畜的生产和品种质量,这些方法是现代的,并且基于对所研究群体的遗传多样性和基因型的更完整的分析,有助于保护分区品种。同时,种群内变异性和良好的适应性应保证奶牛养殖在不同气候带的可持续发展。
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Analysis of polymorphisms in genes associated with milk productivity in red steppe and Caucasian brown cow breeds in two natural‐climate zones (mountains and plain) of Dagestan, Russia
Aim . The unique gene pool of indigenous breeds in the Dagestan Caucasus is threatened by the increasingly widespread use of commercial breeds, which in turn risks narrowing the genetic base necessary to increase and maintain existing genetic diversity. For this reason, our research aimed to study the polymorphism of the PRL, PIT‐1, GH genes in red steppe and Caucasian brown cattle raised in the conditions of Dagestan. Methods . Genotyping of red steppe and Caucasian brown cattle was carried out using PCR‐RFLP methods. Using these methods, genetic analysis was carried out and polymorphism of the prolactin, somatotropin and pituitary‐specific transcription factor genes was studied. Results . As a result of the genotyping of an experimental group of red steppe cattle, it was found that the distribution of two alleles and three genotypes (PIT‐1A and PIT‐1B; PIT‐1AA, PIT‐1BB, PIT‐1AB) of the PIT‐1 gene has a specific character. The specificity of the allelic spectrum of the PRL gene is expressed in the high (0.84) frequency of occurrence of the PRLA allele and low (0.16) frequency of the PRLB allele. This is reflected in the presence of homo‐ and heterozygous genotypes with a frequency of occurrence of 73.0; 23.0; 4.0 %, respectively. Conclusion . Applied genetic approaches to improve the productive and breed qualities of livestock, which are modern and based on a more complete analysis of the genetic diversity and genotype of the studied populations, contribute to the conservation of zoned breeds. At the same time, intrapopulation variability and good adaptability should ensure the sustainable development of dairy cattle breeding in various climatic zones.
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来源期刊
CiteScore
0.80
自引率
50.00%
发文量
73
审稿时长
8 weeks
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