R.A. Healy, C. Truong, M.A. Castellano, G. Bonito, J. Trappe, M.V. Caiafa, A.B. Mujic, E. Nouhra, S. Sánchez-Ramírez, M.E. Smith
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We redescribe two Amylascus species from Australia and describe seven new Amylascus species, five from South America and two from Australia. This is the first report of Amylascus species from South America. We also describe the new South American genus Nothoamylascus as sister lineage to the Pachyphlodes - Amylascus - Luteoamylascus clade (including Amylascus , Luteoamylascus , Pachyphlodes , and Plicariella ). We obtained ITS sequences of mitotic spore mats from Nothoamylascus erubescens gen. & sp. nov. and four of the seven newly described Amylascus species, providing the first evidence of mitotic spore mats in Amylascus . Additional ITS sequences from mitotic spore mats reveal the presence of nine additional undescribed Amylascus and one Nothoamylascus species that do not correspond to any sampled ascomata. We also identify three additional undescribed Amylascus species based on environmental sequences from the feces of two ground-dwelling bird species from Chile, Scelorchilus rubecula and Pteroptochos tarnii . Our results indicate that ascomata from Amylascus and Nothoamylascus species are rarely collected, but molecular data from ectomycorrhizal roots and mitotic spore mats indicate that these species are probably common and widespread in southern South America. Finally, we present a time-calibrated phylogeny that is consistent with a late Gondwanan distribution. The time since the most recent common ancestor of: 1) the family Pezizaceae had a mean of 276 Ma (217–337 HPD); 2) the Amylascus - Pachyphlodes - Nothoamylascus - Luteoamylascus clade had a mean of 79 Ma (60–100 HPD); and 3) the Amylascus - Pachyphlodes clade had a mean of 50 Ma (38–62 HPD). 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引用次数: 0
摘要
淀粉松露(Amylascus)是松露科(Pezizaceae)中外生菌根松露的一个属,已知于澳大利亚,仅有a . herbertianus和a . tasmanicus两种。直链淀粉与北半球的松露(Pachyphlodes, Luteoamylascus)和杯状真菌(Plicariella)密切相关。本文基于新的形态学和分子数据,对南美南部和澳大利亚的直链木及其相关分类群的物种多样性进行了重新评估。我们在梅尔泽试剂中鉴定了以前未记载的子囊孢子颜色、子囊孢子纹饰、膜结构、上皮结构和淀粉样反应的多样性和形态变异。我们重新描述了两个来自澳大利亚的直链淀粉种,并描述了7个新的直链淀粉种,其中5个来自南美洲,2个来自澳大利亚。这是南美洲首次报道的直链淀粉属。我们还将这个新的南美属Nothoamylascus描述为Pachyphlodes - Amylascus - Luteoamylascus分支(包括Amylascus, Luteoamylascus, Pachyphlodes和Plicariella)的姐妹谱系。我们获得了巨紫栎(Nothoamylascus erubescens)有丝分裂孢子席的ITS序列;新发现的7个直链淀粉种中的4个,为直链淀粉种有丝分裂孢子席的存在提供了第一个证据。来自有丝分裂孢子垫的额外ITS序列揭示了另外9个未描述的Amylascus和1个Nothoamylascus物种的存在,这些物种与任何取样的ascomata都不对应。根据智利两种地栖鸟类(Scelorchilus rubecula和Pteroptochos tarnii)粪便的环境序列,我们还鉴定了另外三种未描述的直链蝇。我们的研究结果表明,Amylascus和Nothoamylascus物种的ascomata很少被收集到,但来自外生菌根和有丝分裂孢子的分子数据表明,这些物种可能在南美洲南部普遍存在。最后,我们提出了一个与晚冈瓦纳分布一致的时间校准的系统发育。从最近的共同祖先到现在的时间:1)佩孜科平均276 Ma (217-337 HPD);(2)直链淀粉科-厚叶淀粉科-无直链淀粉科-黄体淀粉科枝平均为79 Ma (60 ~ 100 HPD);3) Amylascus - Pachyphlodes枝平均为50 Ma (38 ~ 62 HPD)。Pachyphlodes的平均树冠年龄为39 Ma (25-42 HPD), Amylascus的平均树冠年龄为28 Ma (20-37 HPD),落在始新世-渐新世边界和南极冰川开始(约35 Ma)附近。
Re-examination of the Southern Hemisphere truffle genus Amylascus (Pezizaceae, Ascomycota) and characterization of the sister genus Nothoamylascus gen. nov.
Amylascus is a genus of ectomycorrhizal truffles within Pezizaceae that is known from Australia and contains only two described species, A. herbertianus and A. tasmanicus . Species of Amylascus are closely related to truffles ( Pachyphlodes , Luteoamylascus ) and cup fungi ( Plicariella ) from the Northern Hemisphere. Here we reevaluate the species diversity of Amylascus and related taxa from southern South America and Australia based on new morphological and molecular data. We identify previously undocumented diversity and morphological variability in ascospore color, ascospore ornamentation, hymenial construction, epithecium structure and the amyloid reaction of the ascus in Melzer’s reagent. We redescribe two Amylascus species from Australia and describe seven new Amylascus species, five from South America and two from Australia. This is the first report of Amylascus species from South America. We also describe the new South American genus Nothoamylascus as sister lineage to the Pachyphlodes - Amylascus - Luteoamylascus clade (including Amylascus , Luteoamylascus , Pachyphlodes , and Plicariella ). We obtained ITS sequences of mitotic spore mats from Nothoamylascus erubescens gen. & sp. nov. and four of the seven newly described Amylascus species, providing the first evidence of mitotic spore mats in Amylascus . Additional ITS sequences from mitotic spore mats reveal the presence of nine additional undescribed Amylascus and one Nothoamylascus species that do not correspond to any sampled ascomata. We also identify three additional undescribed Amylascus species based on environmental sequences from the feces of two ground-dwelling bird species from Chile, Scelorchilus rubecula and Pteroptochos tarnii . Our results indicate that ascomata from Amylascus and Nothoamylascus species are rarely collected, but molecular data from ectomycorrhizal roots and mitotic spore mats indicate that these species are probably common and widespread in southern South America. Finally, we present a time-calibrated phylogeny that is consistent with a late Gondwanan distribution. The time since the most recent common ancestor of: 1) the family Pezizaceae had a mean of 276 Ma (217–337 HPD); 2) the Amylascus - Pachyphlodes - Nothoamylascus - Luteoamylascus clade had a mean of 79 Ma (60–100 HPD); and 3) the Amylascus - Pachyphlodes clade had a mean of 50 Ma (38–62 HPD). The crown age of Pachyphlodes had a mean of 39 Ma (25–42 HPD) and Amylascus had a mean age of 28 Ma (20–37 HPD), falling near the Eocene-Oligocene boundary and the onset of the Antarctic glaciation (c. 35 Ma).
期刊介绍:
Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.