基于表面引发酶聚合耦合铜增强的微rna原子孔电化学检测

TARGETS Pub Date : 2023-10-30 DOI:10.3390/targets1020007
Wenyuan Zhu, Yuzhi Xu, Yanfei Zhang, Si-Yang Liu, Zong Dai, Xiaoyong Zou
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引用次数: 0

摘要

由于miRNAs已被证明在参与许多生物过程中具有不可否认的重要性,因此对其进行灵敏有效的检测具有重要意义。在此,我们提出了一种新的、灵敏的、无标记的电化学miRNA检测方法。该方法采用了三种信号放大技术,包括引物修饰的聚苯乙烯球(PS)与靶miRNA触发的磁珠(mb)的有效共轭,引物上的无模板表面引发酶促聚合(SIEP),以及在方波伏安法(SWV)中使用铜离子检测酸去纯化的引物。与电化学方法相配合,该方法的检出限为120 aM。该方法具有原子级的灵敏度和操作简便,适用于研究和临床的miRNA常规检测。
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Attomole Electrochemical Detection of MicroRNAs Based on Surface-Initiated Enzymatic Polymerization Coupled with Copper Enhancement
The sensitive and effective detection of microRNAs (miRNAs) is of great significance since miRNAs have been proven to have undeniable importance in participating in many biological processes. Herein, we present a novel, sensitive, label-free electrochemical miRNA detection method. Three signal amplification techniques are incorporated in this method, including the efficient conjugate of primer-modified polystyrene spheres (PS) with magnetic beads (MBs) triggered by target miRNA, template-free surface-initiated enzymatic polymerization (SIEP) on the primers, and the use of copper ions in square wave voltammetry (SWV) for detecting acidically depurinated primers. Cooperating with the electrochemical approach, this method was able to achieve a detection limit of 120 aM. With an attomole level of sensitivity and easiness of manipulation, this novel method is suitable for miRNA routine detection in both research and clinical aspects.
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