{"title":"环境DNA (eDNA)检测Ridgway 's rail (Rallus obsoletus)在美国","authors":"Xin Guan, Richard F. Lance, Sheena M. Feist","doi":"10.1007/s12686-023-01321-7","DOIUrl":null,"url":null,"abstract":"Abstract We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail ( Rallus obsoletus ), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Environmental DNA (eDNA) assays for the detection of Ridgway’s rail (Rallus obsoletus) in the United States\",\"authors\":\"Xin Guan, Richard F. Lance, Sheena M. Feist\",\"doi\":\"10.1007/s12686-023-01321-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail ( Rallus obsoletus ), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2023-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s12686-023-01321-7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12686-023-01321-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
摘要设计了两种检测Ridgway ' s Rail (Rallus obsoletus)的新型环境DNA (eDNA)检测方法，并利用该物种在美国已知分布的eDNA样本成功验证了每种检测方法的有效性。这些分析增加了一套可用的工具来监测这种稀有而神秘的沼泽鸟类，并可能有助于进一步阐明其运动模式以及确定重要的迁徙走廊。观察到的检测灵敏度表明了卓越的性能，每次反应的检测限≤8个目标eDNA片段。我们的出版物增加了越来越多的文献支持eDNA调查作为鸟类监测工作的可行工具。
Environmental DNA (eDNA) assays for the detection of Ridgway’s rail (Rallus obsoletus) in the United States
Abstract We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail ( Rallus obsoletus ), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.
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