欧洲鹤耳猴胃肠胰系统部分内分泌细胞的免疫组织化学定位。

Basic and applied histochemistry Pub Date : 1988-01-01
L Castaldo, G Andreozzi, R Antonucci, G Gargiulo
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引用次数: 0

摘要

采用免疫组织化学方法研究了嗜铬粒蛋白A和神经元特异性烯醇化酶(NSE)在神经内分泌肠系统中的分布,以及胃泌素、生长抑素、神经紧张素和维普(VIP)细胞在欧洲猪毛猴(Erinaceus europaeus, GEP)器官中的形态和分布。除食道和胰腺外,嗜铬粒蛋白A和生长抑素免疫反应细胞遍布胃肠道粘膜。胃泌素细胞是幽门腺、十二指肠粘膜和小肠神经紧张素细胞所特有的细胞。GEP系统未见VIP免疫反应性内分泌细胞。在GEP装置的神经细胞体和细胞壁末端均检测到VIP和NSE的免疫反应。胃底和幽门黏膜内分泌细胞均有NSE免疫反应。
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Immunohistochemical localization of some endocrine cells in the gastroenteropancreatic system of Erinaceus europaeus.

The distribution of chromogranin A and neuron specific enolase (NSE) in the neuroendocrine gut system and the morphology and distribution of cells containing gastrin, somatostatin, neurotensin and VIP in the gastroenteropacreatic (GEP) apparatus of Erinaceus europaeus were investigated by immunohistochemical methods. Chromogranin A and somatostatin immunoreactive cells were present throughout the gastrointestinal mucosa, with the exception of the oesophagus and in the pancreas. Gastrin cells were peculiar of the pyloric glands and duodenal mucosa and neurotensin cells of the small intestine. No VIP immunoreactive endocrine cells were noticed in the GEP system. VIP and NSE immunoreactivities were detected both in nerve cell bodies and terminals of the wall of the GEP apparatus. NSE immunoreactivity was found in the endocrine cells of the fundic and pyloric mucosa.

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