姜蒜醇提取物对井水中金黄色葡萄球菌生物膜形成的影响

None George, P. A., None Akani, N. P., None Ugboma, C. J.
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摘要

本研究研究了生姜和大蒜对河流州哈科特港市地方政府区选定井中分离的金黄色葡萄球菌生物成膜的影响。从随机选取的4个钻孔中采集24份水样。Borikiri产生生物膜的菌株数量最多,而elelenwo产生生物膜的菌株数量最少。在23株金黄色葡萄球菌分离株中,14株生物膜生成阳性。这些分离株的溶血产物范围为α、β和γ。用不同浓度的植物提取物接种滤纸圆盘。与甲醇姜提取物相比,100mg/ml甲醇大蒜提取物对Borikiri的抑制区范围最大,而与甲醇姜提取物相比,100mg/ml甲醇大蒜提取物对Elelenwo的抑制区范围没有变化。本研究提示,甲醇姜蒜提取物可作为钻孔水中潜在的抗生物膜剂,降低细菌生物膜的形成风险。应加强监测系统,以评估疾病的危险因素,并提供预防和保护公众健康的战略。
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Effects of Methanolic Ginger and Garlic Extracts on Biofilm Forming Staphylococcus aureus Isolated from Borehole Water
This study investigated the effect of Zingiber officinale (Ginger) and Allum sativum (Garlic) on biofilm forming Staphylococcus aureus isolated from selected boreholes in Port Harcourt city Local Government Area of Rivers State. Twenty four water samples were collected from 4 randomly selected borehole. Borikiri had the highest number of isolates that produced biofilm, while Elelennwo had the lowest number. Out of 23 isolates of Staphylococcus aureus, 14 were positive for biofilm production. Hemolysis production range of these isolates was from alpha, beta and gamma. Filter paper disc were inoculated with various plant extract concentrations. Methanol garlic extract at 100mg/ml had the highest range of zone of inhibition at Borikiri when compared to methanol ginger extract while methanol garlic extract at 100mg/ml had no range at Elelenwo when compared to methanol ginger extract. This study suggests that methanolic ginger and garlic extracts can be used as a potential anti-biofilm agent in borehole water, to reduce the risk of bacterial biofilm formation. Surveillance systems should be increased for assessing risk factors of diseases and to provide strategies to prevent and protect public health.
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