{"title":"皂苷与二甲双胍联合治疗可改善2型糖尿病大鼠的生化状态","authors":"Karim EL-Said, Yomna Elbarbary, Amro Mohamed","doi":"10.21608/bbj.2023.321886","DOIUrl":null,"url":null,"abstract":"Saponins (Sap) are effective bioactive compounds for ameliorating various diseases complications. This study addressed the effects of co-treatment with Sap and metformin (Met) on the biochemical status of type 2 diabetes mellitus (T2-DM) rats. Forty male Sprague-Dawley rats were divided into 5 groups (n = 8) as follows: group 1 (Gp1) was the negative control group. Gp2 was fed on a high fat diet (HFD) for 8 weeks (wks) and received a single intraperitoneal (i.p) injection of 30 mg/kg streptozotocin (STZ) after 8 wks to induce T2-DM. Gp3 was treated as Gp2, after 8 wks rats received 250 mg/kg Met by oral gavage daily for 4 weeks. Gp4 was treated as Gp2, and after 8 wks rats received 300 mg/kg Sap by oral gavage daily for 4 weeks. Gp5 was treated as Gp2, after the 8 wks rats received it, and then co-treated with Met as in Gp3 and Sap as in Gp4. The percentage of body weight (% b.wt) changes, hematological, and biochemical parameters were determined. The results showed that co-treatment with Met and Sap led to significant improvement in the % b.wt of T2-DM rats. Co-treatment of T2-DM rats with Met and Sap led to synergistic effects in the reduction of serum glucose levels and an increase in the C-peptide levels. Co-treatment of T2-DM rats with Met and Sap led to significant improvements in the hepato-renal functions and antioxidant status. Sap could play an important role in regulating T2-DM and can be developed as a promising natural material for diabetes management.","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Co-treatment with saponin and metformin improves the biochemical status of type 2 diabetes mellitus rats\",\"authors\":\"Karim EL-Said, Yomna Elbarbary, Amro Mohamed\",\"doi\":\"10.21608/bbj.2023.321886\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Saponins (Sap) are effective bioactive compounds for ameliorating various diseases complications. This study addressed the effects of co-treatment with Sap and metformin (Met) on the biochemical status of type 2 diabetes mellitus (T2-DM) rats. Forty male Sprague-Dawley rats were divided into 5 groups (n = 8) as follows: group 1 (Gp1) was the negative control group. Gp2 was fed on a high fat diet (HFD) for 8 weeks (wks) and received a single intraperitoneal (i.p) injection of 30 mg/kg streptozotocin (STZ) after 8 wks to induce T2-DM. Gp3 was treated as Gp2, after 8 wks rats received 250 mg/kg Met by oral gavage daily for 4 weeks. Gp4 was treated as Gp2, and after 8 wks rats received 300 mg/kg Sap by oral gavage daily for 4 weeks. Gp5 was treated as Gp2, after the 8 wks rats received it, and then co-treated with Met as in Gp3 and Sap as in Gp4. The percentage of body weight (% b.wt) changes, hematological, and biochemical parameters were determined. The results showed that co-treatment with Met and Sap led to significant improvement in the % b.wt of T2-DM rats. Co-treatment of T2-DM rats with Met and Sap led to synergistic effects in the reduction of serum glucose levels and an increase in the C-peptide levels. Co-treatment of T2-DM rats with Met and Sap led to significant improvements in the hepato-renal functions and antioxidant status. Sap could play an important role in regulating T2-DM and can be developed as a promising natural material for diabetes management.\",\"PeriodicalId\":92047,\"journal\":{\"name\":\"International biological and biomedical journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International biological and biomedical journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/bbj.2023.321886\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International biological and biomedical journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/bbj.2023.321886","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Co-treatment with saponin and metformin improves the biochemical status of type 2 diabetes mellitus rats
Saponins (Sap) are effective bioactive compounds for ameliorating various diseases complications. This study addressed the effects of co-treatment with Sap and metformin (Met) on the biochemical status of type 2 diabetes mellitus (T2-DM) rats. Forty male Sprague-Dawley rats were divided into 5 groups (n = 8) as follows: group 1 (Gp1) was the negative control group. Gp2 was fed on a high fat diet (HFD) for 8 weeks (wks) and received a single intraperitoneal (i.p) injection of 30 mg/kg streptozotocin (STZ) after 8 wks to induce T2-DM. Gp3 was treated as Gp2, after 8 wks rats received 250 mg/kg Met by oral gavage daily for 4 weeks. Gp4 was treated as Gp2, and after 8 wks rats received 300 mg/kg Sap by oral gavage daily for 4 weeks. Gp5 was treated as Gp2, after the 8 wks rats received it, and then co-treated with Met as in Gp3 and Sap as in Gp4. The percentage of body weight (% b.wt) changes, hematological, and biochemical parameters were determined. The results showed that co-treatment with Met and Sap led to significant improvement in the % b.wt of T2-DM rats. Co-treatment of T2-DM rats with Met and Sap led to synergistic effects in the reduction of serum glucose levels and an increase in the C-peptide levels. Co-treatment of T2-DM rats with Met and Sap led to significant improvements in the hepato-renal functions and antioxidant status. Sap could play an important role in regulating T2-DM and can be developed as a promising natural material for diabetes management.