Pub Date : 2023-10-01DOI: 10.21608/bbj.2023.321880
Mohamed Elbakry, Wesam Elremaly
ABSTRACT
{"title":"Antidiabetic activity of some common medicinal plants","authors":"Mohamed Elbakry, Wesam Elremaly","doi":"10.21608/bbj.2023.321880","DOIUrl":"https://doi.org/10.21608/bbj.2023.321880","url":null,"abstract":"ABSTRACT","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bbj.2023.321886
Karim EL-Said, Yomna Elbarbary, Amro Mohamed
Saponins (Sap) are effective bioactive compounds for ameliorating various diseases complications. This study addressed the effects of co-treatment with Sap and metformin (Met) on the biochemical status of type 2 diabetes mellitus (T2-DM) rats. Forty male Sprague-Dawley rats were divided into 5 groups (n = 8) as follows: group 1 (Gp1) was the negative control group. Gp2 was fed on a high fat diet (HFD) for 8 weeks (wks) and received a single intraperitoneal (i.p) injection of 30 mg/kg streptozotocin (STZ) after 8 wks to induce T2-DM. Gp3 was treated as Gp2, after 8 wks rats received 250 mg/kg Met by oral gavage daily for 4 weeks. Gp4 was treated as Gp2, and after 8 wks rats received 300 mg/kg Sap by oral gavage daily for 4 weeks. Gp5 was treated as Gp2, after the 8 wks rats received it, and then co-treated with Met as in Gp3 and Sap as in Gp4. The percentage of body weight (% b.wt) changes, hematological, and biochemical parameters were determined. The results showed that co-treatment with Met and Sap led to significant improvement in the % b.wt of T2-DM rats. Co-treatment of T2-DM rats with Met and Sap led to synergistic effects in the reduction of serum glucose levels and an increase in the C-peptide levels. Co-treatment of T2-DM rats with Met and Sap led to significant improvements in the hepato-renal functions and antioxidant status. Sap could play an important role in regulating T2-DM and can be developed as a promising natural material for diabetes management.
{"title":"Co-treatment with saponin and metformin improves the biochemical status of type 2 diabetes mellitus rats","authors":"Karim EL-Said, Yomna Elbarbary, Amro Mohamed","doi":"10.21608/bbj.2023.321886","DOIUrl":"https://doi.org/10.21608/bbj.2023.321886","url":null,"abstract":"Saponins (Sap) are effective bioactive compounds for ameliorating various diseases complications. This study addressed the effects of co-treatment with Sap and metformin (Met) on the biochemical status of type 2 diabetes mellitus (T2-DM) rats. Forty male Sprague-Dawley rats were divided into 5 groups (n = 8) as follows: group 1 (Gp1) was the negative control group. Gp2 was fed on a high fat diet (HFD) for 8 weeks (wks) and received a single intraperitoneal (i.p) injection of 30 mg/kg streptozotocin (STZ) after 8 wks to induce T2-DM. Gp3 was treated as Gp2, after 8 wks rats received 250 mg/kg Met by oral gavage daily for 4 weeks. Gp4 was treated as Gp2, and after 8 wks rats received 300 mg/kg Sap by oral gavage daily for 4 weeks. Gp5 was treated as Gp2, after the 8 wks rats received it, and then co-treated with Met as in Gp3 and Sap as in Gp4. The percentage of body weight (% b.wt) changes, hematological, and biochemical parameters were determined. The results showed that co-treatment with Met and Sap led to significant improvement in the % b.wt of T2-DM rats. Co-treatment of T2-DM rats with Met and Sap led to synergistic effects in the reduction of serum glucose levels and an increase in the C-peptide levels. Co-treatment of T2-DM rats with Met and Sap led to significant improvements in the hepato-renal functions and antioxidant status. Sap could play an important role in regulating T2-DM and can be developed as a promising natural material for diabetes management.","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136198367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bbj.2023.321887
El-Sayyedah Khadijah Ayyad, Ramadan Kandyle, Mohamed Salem
Applications of silver nanoparticles (AgNPs) include biosensing, cosmetics, and medicine. AgNPs may become deposited as particles in many tissues and vital organs after exposure. AgNPs toxicity could be removed from the body using natural products. This study aimed to address the effective of Phoenix dactylifera seed extract (PDSE) in treating renal and lipid profile alterations brought on by the exposure to AgNPs. The following four groups (n=10) of male CD1 mice were created: 200 µl of sterile saline has been injected intraperitoneal (i.p) into group 1 (Gp1). Gp2 had received PDSE (100 mg/kg) i.p. Gp3 had received 0.25 mg/Kg of AgNPs, i.p. every day for a month. Gp4 had received both AgNPs and PDSE treatment, as in Gp2 and Gp3. The kidney's histological changes and lipid profile were investigated. AgNPs caused a noticeable histological change in the kidney tissues as well as significant changes in the lipid profile. PDSE treatment after AgNPs injection reduced the toxicities on the kidneys` tissues and lipid profile. Together, PDSE treatment could be employed to decline the negative impacts that induced by AgNPs exposure on kidneys` tissues and lipid profile.
{"title":"Phoenix dactylifera seeds extract ameliorates the lipid profile fluctuations and the renal toxicity induced by silver nanoparticles in mice","authors":"El-Sayyedah Khadijah Ayyad, Ramadan Kandyle, Mohamed Salem","doi":"10.21608/bbj.2023.321887","DOIUrl":"https://doi.org/10.21608/bbj.2023.321887","url":null,"abstract":"Applications of silver nanoparticles (AgNPs) include biosensing, cosmetics, and medicine. AgNPs may become deposited as particles in many tissues and vital organs after exposure. AgNPs toxicity could be removed from the body using natural products. This study aimed to address the effective of Phoenix dactylifera seed extract (PDSE) in treating renal and lipid profile alterations brought on by the exposure to AgNPs. The following four groups (n=10) of male CD1 mice were created: 200 µl of sterile saline has been injected intraperitoneal (i.p) into group 1 (Gp1). Gp2 had received PDSE (100 mg/kg) i.p. Gp3 had received 0.25 mg/Kg of AgNPs, i.p. every day for a month. Gp4 had received both AgNPs and PDSE treatment, as in Gp2 and Gp3. The kidney's histological changes and lipid profile were investigated. AgNPs caused a noticeable histological change in the kidney tissues as well as significant changes in the lipid profile. PDSE treatment after AgNPs injection reduced the toxicities on the kidneys` tissues and lipid profile. Together, PDSE treatment could be employed to decline the negative impacts that induced by AgNPs exposure on kidneys` tissues and lipid profile.","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bbj.2023.321883
Wesam Salama, Abdelwahab El-Newahy, Soha Sakr, Nabila El-desoky
ABSTRACT
{"title":"Antitumor efficacy of the Egyptian scorpion Leiurus quinquestriatus whole body extract in EAC-bearing mice","authors":"Wesam Salama, Abdelwahab El-Newahy, Soha Sakr, Nabila El-desoky","doi":"10.21608/bbj.2023.321883","DOIUrl":"https://doi.org/10.21608/bbj.2023.321883","url":null,"abstract":"ABSTRACT","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136198378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bbj.2023.321882
Nabila El-Desouki, Mohamed Salem, Dalia Afifi, Mohamed Nassef, Faten Abdallah
The present study aims to study the effect of vitamin D (Vit. D) or/and coconut oil (C.O) on the splenic histological changes of diabetic adult mice induced by streptozotocin (STZ). The mice were divided into 7 groups (n=10), and the experimental duration was 4 weeks. Group 1 (GpI): control group without any treatment; GpII and GpIII: non-diabetic groups, orally received Vit. D in a dose of 500 IU (6.25 ml)/kg b.w/d or C.O in a dose of 7.5 ml /kg b.w/d ,respectively; GpIV: diabetic group injected i.p. with a single dose of STZ (200 mg/kg bw). Gps V, VI and VII: administration of Vit. D or C.O or both together to diabetic group respectively. The results showed no significant changes in the blood glucose (BG), insulin, and splenic weight in GpII and GpIII. GpIV showed a significant increase in BG, significant decrease in insulin levels, and splenic weight values. GpV recorded a moderate decrease in BG, and a moderate increase in insulin levels and splenic weight atrophy. While GpVI or GpVII showed a marked decrease in BG and an increase in insulin levels and splenic weight increment. Histologically, the splenic sections of control or non-diabetic mice received either Vit. D or C.O showed normal structure of the splenocytes. GpIV showed great numbers of giant cells, disarrangement, interference of red and white pulps, and dilated congested blood vessels. Mild improvement was seen in GpV, while in GpVI or GpVII showed a marked improvement in the splenic tissues. In conclusion, diabetic mice received Vit. D/C. O showed strong anti-hyperglycemic effects to restore the glucose and insulin levels close to normal levels. It also restored the splenic weight and histological architecture close to normal status than those administered Vit. D alone.
{"title":"The helpful effect of vitamin D and coconut oil in modulating the histological disorders of the splenic tissue in hyperglycemic mice","authors":"Nabila El-Desouki, Mohamed Salem, Dalia Afifi, Mohamed Nassef, Faten Abdallah","doi":"10.21608/bbj.2023.321882","DOIUrl":"https://doi.org/10.21608/bbj.2023.321882","url":null,"abstract":"The present study aims to study the effect of vitamin D (Vit. D) or/and coconut oil (C.O) on the splenic histological changes of diabetic adult mice induced by streptozotocin (STZ). The mice were divided into 7 groups (n=10), and the experimental duration was 4 weeks. Group 1 (GpI): control group without any treatment; GpII and GpIII: non-diabetic groups, orally received Vit. D in a dose of 500 IU (6.25 ml)/kg b.w/d or C.O in a dose of 7.5 ml /kg b.w/d ,respectively; GpIV: diabetic group injected i.p. with a single dose of STZ (200 mg/kg bw). Gps V, VI and VII: administration of Vit. D or C.O or both together to diabetic group respectively. The results showed no significant changes in the blood glucose (BG), insulin, and splenic weight in GpII and GpIII. GpIV showed a significant increase in BG, significant decrease in insulin levels, and splenic weight values. GpV recorded a moderate decrease in BG, and a moderate increase in insulin levels and splenic weight atrophy. While GpVI or GpVII showed a marked decrease in BG and an increase in insulin levels and splenic weight increment. Histologically, the splenic sections of control or non-diabetic mice received either Vit. D or C.O showed normal structure of the splenocytes. GpIV showed great numbers of giant cells, disarrangement, interference of red and white pulps, and dilated congested blood vessels. Mild improvement was seen in GpV, while in GpVI or GpVII showed a marked improvement in the splenic tissues. In conclusion, diabetic mice received Vit. D/C. O showed strong anti-hyperglycemic effects to restore the glucose and insulin levels close to normal levels. It also restored the splenic weight and histological architecture close to normal status than those administered Vit. D alone.","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bbj.2023.321884
Zeinab Attia, Hala Mostafa, Walaa Dawood
This study examined the effect of a commercial probiotic ( Lactobacillus acidophilus ) on oxidative stress induced by different levels of stocking density in Nile tilapia fingerlings. Fish were divided into 6 groups: group1: control (90 fish/m 3 ), group 2: control (90 fish/m 3 ) with probiotic, group 3: high stocking density Ι (150 fish/m 3 ), group 4: high stocking density Ι (150 fish/m 3 ) with probiotic, group 5: high stocking density П (300 fish/m 3 ), group 6: high stocking density П (300 fish/m 3 ) with probiotic for 30 days. The results showed increase in the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) by increased stocking density compared with their respective control. Activities of AST and ALT at high stocking density Ι, П with addition of probiotic decreased compared to their respective high stocking densities in liver and white muscles. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione S-transferase (GST) decreased by increasing stocking density with compared with their respective control in liver and white muscle. However, they increased significantly in high stocking density Ι, П with probiotic compared with high stocking density Ι, П. The xanthine oxidase (XO) activity and malondialdehyde (MDA) level increased by increasing stocking density compared with their respective control and decreased in high stocking density Ι, П with addition of probiotic compared with high stocking density Ι, П. The data concluded that probiotics enhanced the oxidative stress caused by high stocking density in liver and white muscles in Oreochromis niloticus .
{"title":"Effect of a commercial probiotic on oxidative stress induced by high stocking density in nile tilapia fingerlings","authors":"Zeinab Attia, Hala Mostafa, Walaa Dawood","doi":"10.21608/bbj.2023.321884","DOIUrl":"https://doi.org/10.21608/bbj.2023.321884","url":null,"abstract":"This study examined the effect of a commercial probiotic ( Lactobacillus acidophilus ) on oxidative stress induced by different levels of stocking density in Nile tilapia fingerlings. Fish were divided into 6 groups: group1: control (90 fish/m 3 ), group 2: control (90 fish/m 3 ) with probiotic, group 3: high stocking density Ι (150 fish/m 3 ), group 4: high stocking density Ι (150 fish/m 3 ) with probiotic, group 5: high stocking density П (300 fish/m 3 ), group 6: high stocking density П (300 fish/m 3 ) with probiotic for 30 days. The results showed increase in the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) by increased stocking density compared with their respective control. Activities of AST and ALT at high stocking density Ι, П with addition of probiotic decreased compared to their respective high stocking densities in liver and white muscles. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione S-transferase (GST) decreased by increasing stocking density with compared with their respective control in liver and white muscle. However, they increased significantly in high stocking density Ι, П with probiotic compared with high stocking density Ι, П. The xanthine oxidase (XO) activity and malondialdehyde (MDA) level increased by increasing stocking density compared with their respective control and decreased in high stocking density Ι, П with addition of probiotic compared with high stocking density Ι, П. The data concluded that probiotics enhanced the oxidative stress caused by high stocking density in liver and white muscles in Oreochromis niloticus .","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microparticles (MPs) are well recognized as a global concern arising from plastic waste. MPs can cause various environmental and health problems. This study was conducted to evaluate the effect of the administrations with MPs for short and long terms on the pathophysiological and histological status of hepatorenal and intestinal tissues in mice. Forty (40) male CD-1 mice were divided into 4 groups (n = 10) as follows; the first group (Gp1) was served as a negative control, administered orally with 200 µl of dist. H 2 O. Gp2 was administrated orally daily with MPs (66.4 mg/kg b. wt) for 28 days. Gp3 was administered as in Gp1 for 120 days and used as a negative control for Gp4. Gp4 was administrated with MPs as in Gp2 orally for 120 days. All groups were sacrificed, and blood samples, liver, kidney, and intestinal tissue samples were collected for haematological, biochemical, and histopathological analysis. The results showed that the short-term administration of MPs did not show significant changes on the haematological, biochemical parameters, histological investigations of the hepatorenal and intestinal tissues, however, administration for long term led to significant changes pathophysiological and histopathological alterations in the liver, kidney, and intestinal tissues. In conclusion, administration of MPs for long term could be harmful on the vital organs
{"title":"Long-term administrations of microplastics induces hepatorenal and intestinal tissues damages in experimental mice","authors":"Ghada Tabl, Sabry El-Naggar, Nabila El-Desouki, Hadeer Elmorsi","doi":"10.21608/bbj.2023.321885","DOIUrl":"https://doi.org/10.21608/bbj.2023.321885","url":null,"abstract":"Microparticles (MPs) are well recognized as a global concern arising from plastic waste. MPs can cause various environmental and health problems. This study was conducted to evaluate the effect of the administrations with MPs for short and long terms on the pathophysiological and histological status of hepatorenal and intestinal tissues in mice. Forty (40) male CD-1 mice were divided into 4 groups (n = 10) as follows; the first group (Gp1) was served as a negative control, administered orally with 200 µl of dist. H 2 O. Gp2 was administrated orally daily with MPs (66.4 mg/kg b. wt) for 28 days. Gp3 was administered as in Gp1 for 120 days and used as a negative control for Gp4. Gp4 was administrated with MPs as in Gp2 orally for 120 days. All groups were sacrificed, and blood samples, liver, kidney, and intestinal tissue samples were collected for haematological, biochemical, and histopathological analysis. The results showed that the short-term administration of MPs did not show significant changes on the haematological, biochemical parameters, histological investigations of the hepatorenal and intestinal tissues, however, administration for long term led to significant changes pathophysiological and histopathological alterations in the liver, kidney, and intestinal tissues. In conclusion, administration of MPs for long term could be harmful on the vital organs","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ketan Thanki, Michael E Nicholls, Aakash Gajjar, Anthony J Senagore, Suimin Qiu, Csaba Szabo, Mark R Hellmich, Celia Chao
The colorectal cancer (CRC) Subtyping Consortium has unified six independent molecular classification systems, based on gene expression data, into a single consensus system with four distinct groups, known as the Consensus Molecular Subtypes (CMS); clinical implications are discussed in this review. This article is based on a literature review relevant to the CMS of CRC indexed in PubMed (US National Library of Medicine) as well as the authors' own published data. The CMS were determined and correlated with epigenomic, transcriptomic, microenvironmental, genetic, prognostic and clinical characteristics. The CMS1 subtype is immunogenic and hypermutated. CMS2 tumors are activated by the WNT-β-catenin pathway and have the highest overall survival. CMS3 feature a metabolic cancer phenotype and CMS4 cancers have the worst survival and have a strong stromal gene signature. The Consensus Molecular Subtypes of CRC may better inform clinicians of prognosis, therapeutic response, and potential novel therapeutic strategies.
{"title":"Consensus Molecular Subtypes of Colorectal Cancer and their Clinical Implications.","authors":"Ketan Thanki, Michael E Nicholls, Aakash Gajjar, Anthony J Senagore, Suimin Qiu, Csaba Szabo, Mark R Hellmich, Celia Chao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The colorectal cancer (CRC) Subtyping Consortium has unified six independent molecular classification systems, based on gene expression data, into a single consensus system with four distinct groups, known as the Consensus Molecular Subtypes (CMS); clinical implications are discussed in this review. This article is based on a literature review relevant to the CMS of CRC indexed in PubMed (US National Library of Medicine) as well as the authors' own published data. The CMS were determined and correlated with epigenomic, transcriptomic, microenvironmental, genetic, prognostic and clinical characteristics. The CMS1 subtype is immunogenic and hypermutated. CMS2 tumors are activated by the WNT-β-catenin pathway and have the highest overall survival. CMS3 feature a metabolic cancer phenotype and CMS4 cancers have the worst survival and have a strong stromal gene signature. The Consensus Molecular Subtypes of CRC may better inform clinicians of prognosis, therapeutic response, and potential novel therapeutic strategies.</p>","PeriodicalId":92047,"journal":{"name":"International biological and biomedical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557054/pdf/nihms888351.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35336220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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