Assessment of the glutathione system and the biomolecules oxidation products contribution to the state of free radical homeostasis in women of two ethnic groups with insomnia

Own and literature data showed a change in the free radical oxidation products and indicators of the glutathione system concentration in menopausal women with insomnia. The change in the studied parameters depended on the ethnicity of the patients. The aim of the study was to assess the informative value of these indicators and their contribution to the state of free radical homeostasis in menopausal women of Russian and Buryat ethnic groups with insomnia. Material and methods. The study involved women of the menopausal period of the Russian (n = 136) and Buryat ethnos (n = 94). The women underwent a general clinical examination and a questionnaire. Then they were divided into climacteric phases, control groups and groups with insomnia. Enzyme immunoassay, spectrophotometric and fluorometric methods of analysis were used to determine values of glutathione system and biomolecule oxidation products. The informative value of the studied indicators and their contribution to the state of free-radical homeostasis was assessed with the help of discriminant analysis. Results and discussion. The most informative indicators in the group of Russian women with insomnia in perimenopause were content of advanced glycation end products (AGEs), advanced oxidation protein products (AOPP), products of DNA oxidative modification, oxidized glutathione, glutathione reductase activity; in postmenopause – concentration of AOPP, products of DNA oxidative modification, and glutathione reductase activity. In Buryat perimenopausal women the most informative values were content of AGEs, of reduced and oxidized glutathione and their ratio, and in postmenopause – content of AGEs, of oxidized glutathione and glutathione reductase activity. Mathematical models have been developed that demonstrate the prevalence of the redox balance prooxidant component contribution in Russian ethnic group and the antioxidant component in Buryat ethnic group and allow characterizing patients with insomnia with high accuracy depending on the phase of menopause and ethnicity. Conclusions. In women of the Russian ethnic group with insomnia in the menopausal period, the greatest contribution to the state of free radical homeostasis is made by the products of oxidation of biomolecules, which characterizes the predominance of the processes of disadaptation. In women of the Buryat ethnic group, in response to insomnia in the climacteric period, the components of the glutathione system make the greatest contribution to the state of free radical homeostasis, which characterizes the activation of adaptive capabilities throughout the climacteric period.