Evaluation of Phytochemical Properties and Antioxidant Activity of Methanol Extract of Ziziphus Spina-Christi, Satureja Khuzistanica, and Salvia Rosmarinus Using FT-IR Method

Introduction: Medicinal plants have shown special application in the pharmaceutical and food industries. Chemical analysis and determination of medicinal compounds of medicinal plants attract great attention throughout the world. The present study was designed to evaluate the phytochemical properties and antioxidant activity of methanol extract of three species of aromatic plants by FTIR method. Methods: Three species of aromatic plants including Konar (Ziziphus spina-christi), rosemary (Salvia rosmarinus), and Marzeh khuzestani (Satureja khuzistanica) were prepared and studied from the cities of Dehlran and Ilam locating in the west of Iran. These medicinal plants were dried and then powdered. The active groups of the medicinal plants were identified by the FT-IR method. Total antioxidant capacity was measured based on divalent iron reducing ability (FRAP) and by a single electron transfer mechanism. Results: The FT-IR results displayed that Z. spina-christi surrounds 11 functional groups, rosemary has 13 functional groups, and Khuzestani pepper contains 12 functional groups. The antioxidant potential of the methanolic extract of Z. spina-christi was 7 mmol Fe2+/L, rosemary was 1.93 mmol Fe2+/L, and Marzeh khuzestani was 12.99 mmol Fe2+/L. Conclusion: Ziziphus spina-christi, Salvia rosmarinus, and Satureja Khuzestani have strong antioxidant properties due to their chemical compounds and can be used for medicinal purposes.