Trypsin as a New Marker of Metabolism in Animals

Trypsin is produced by the pancreas and hydrolyzes food protein to polypeptides and amino acids in the intestine. However, the function of the enzyme extends far beyond the digestive organs, as it is involved in the regulation of blood pressure, inflammatory reactions, blood clotting, and pancreatic function. Trypsin-activated PAR receptors have been found to affect cellular processes in the body. The effect of trypsin on animal metabolism has not been studied before. Therefore, the aim of the present study was to compare trypsin activity in different animals and in laying hens of different productivity to gain new knowledge about the signaling role of trypsin in the regulation of metabolism. Results of studies on cows, goats and poultry showed that the maximum trypsin activity in blood serum was observed in broiler chickens, which exceeded the level of laying hens by 385.4% for cows, by 89.4% for goats and by 22.6% for laying hens. An analysis of the enzyme activity in the blood of laying hens has identified the hens capable of laying eggs compared to those that have not yet laid eggs. Calculation of the correlation indicates a strong positive relationship between egg-laying and trypsin activity. Consequently, trypsin activity can be used as an indicator of metabolic processes in animals.