单纯疱疹病毒(HSV)免疫检测技术的比较研究

S Meguenni, D Bandoui, Y Lahlou, J El Kolli, D Benyakoub, A Bouguermouh
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引用次数: 0

摘要

比较了间接免疫荧光(IF)、免疫过氧化物酶(IP)和血清中和试验对单纯疱疹病毒(HSV)的检测和鉴定,直接用于组织涂片或接种临床材料的细胞。利用兔免疫球蛋白与异源病毒抗原交叉吸收,获得了两种HSV血清型的检测和明确区分。特异性单克隆抗体的免疫荧光染色与标准Vero细胞培养一样敏感且更快速,用于HSV的实验室诊断。
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[Comparative study of immunologic techniques applied to the detection of herpes simplex virus (HSV)].

Indirect immunofluorescence (IF), immunoperoxidase (IP) and seroneutralization tests were compared for detection and identification or Herpes simplex virus (HSV), directly in tissue smears or in cells inoculated with clinical materials. Detection and clear differentiation between the two HSV serotypes were obtained using rabbit immunoglobulin cross-absorbed with heterologous virus antigen. Immunofluorescent staining with specific monoclonal antibodies is as sensitive and more rapid than standard Vero cell cultures, for the laboratory diagnosis of HSV.

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