厚生物样品中的三维显微镜:一种调整焦点和校正球差的新方法

Z Kam, D A Agard, J W Sedat
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引用次数: 0

摘要

设计了一种用于光学显微镜的改进光学系统,以便远程移动焦平面并操纵任何给定物镜的点扩展函数。在显微镜管中插入一个可调节的望远镜系统,以移动中间图像的位置,从而实现生物样品三维成像的两个重要目标。首先,它允许焦点快速变化,而无需实际移动物镜。这允许活体标本的高通量三维显微镜。其次,使物镜球差补偿成为可能。当利用高数值孔径物镜对厚样品进行成像时,这种畸变尤为显著。
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Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration

A modified optical system for the light microscope has been devised in order to remotely shift the focal plane and to manipulate the point spread function for any given objective lens. An adjustable telescope system is inserted into the microscope tube so as to move the intermediate image position, thus achieving two goals of fundamental importance for the three-dimensional imaging of biological samples. First, it allows the focus to be rapidly varied without actually moving the objective lens. This permits high throughput three-dimensional microscopy of living specimens. Secondly, it makes possible the compensation of objective lens spherical aberration. This distortion is especially significant when high numerical aperture objectives are utilized to image deep into thick specimens.

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