厚生物样品中的三维显微镜:一种调整焦点和校正球差的新方法

Z Kam, D A Agard, J W Sedat
{"title":"厚生物样品中的三维显微镜:一种调整焦点和校正球差的新方法","authors":"Z Kam,&nbsp;D A Agard,&nbsp;J W Sedat","doi":"10.1002/1361-6374(199703)5:1<40::AID-BIO4>3.0.CO;2-W","DOIUrl":null,"url":null,"abstract":"<p>A modified optical system for the light microscope has been devised in order to remotely shift the focal plane and to manipulate the point spread function for any given objective lens. An adjustable telescope system is inserted into the microscope tube so as to move the intermediate image position, thus achieving two goals of fundamental importance for the three-dimensional imaging of biological samples. First, it allows the focus to be rapidly varied without actually moving the objective lens. This permits high throughput three-dimensional microscopy of living specimens. Secondly, it makes possible the compensation of objective lens spherical aberration. This distortion is especially significant when high numerical aperture objectives are utilized to image deep into thick specimens.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"5 1","pages":"40-49"},"PeriodicalIF":0.0000,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199703)5:1<40::AID-BIO4>3.0.CO;2-W","citationCount":"0","resultStr":"{\"title\":\"Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration\",\"authors\":\"Z Kam,&nbsp;D A Agard,&nbsp;J W Sedat\",\"doi\":\"10.1002/1361-6374(199703)5:1<40::AID-BIO4>3.0.CO;2-W\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>A modified optical system for the light microscope has been devised in order to remotely shift the focal plane and to manipulate the point spread function for any given objective lens. An adjustable telescope system is inserted into the microscope tube so as to move the intermediate image position, thus achieving two goals of fundamental importance for the three-dimensional imaging of biological samples. First, it allows the focus to be rapidly varied without actually moving the objective lens. This permits high throughput three-dimensional microscopy of living specimens. Secondly, it makes possible the compensation of objective lens spherical aberration. This distortion is especially significant when high numerical aperture objectives are utilized to image deep into thick specimens.</p>\",\"PeriodicalId\":100176,\"journal\":{\"name\":\"Bioimaging\",\"volume\":\"5 1\",\"pages\":\"40-49\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-05-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/1361-6374(199703)5:1<40::AID-BIO4>3.0.CO;2-W\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioimaging\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199703%295%3A1%3C40%3A%3AAID-BIO4%3E3.0.CO%3B2-W\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199703%295%3A1%3C40%3A%3AAID-BIO4%3E3.0.CO%3B2-W","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

设计了一种用于光学显微镜的改进光学系统,以便远程移动焦平面并操纵任何给定物镜的点扩展函数。在显微镜管中插入一个可调节的望远镜系统,以移动中间图像的位置,从而实现生物样品三维成像的两个重要目标。首先,它允许焦点快速变化,而无需实际移动物镜。这允许活体标本的高通量三维显微镜。其次,使物镜球差补偿成为可能。当利用高数值孔径物镜对厚样品进行成像时,这种畸变尤为显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration

A modified optical system for the light microscope has been devised in order to remotely shift the focal plane and to manipulate the point spread function for any given objective lens. An adjustable telescope system is inserted into the microscope tube so as to move the intermediate image position, thus achieving two goals of fundamental importance for the three-dimensional imaging of biological samples. First, it allows the focus to be rapidly varied without actually moving the objective lens. This permits high throughput three-dimensional microscopy of living specimens. Secondly, it makes possible the compensation of objective lens spherical aberration. This distortion is especially significant when high numerical aperture objectives are utilized to image deep into thick specimens.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Magnetic Particle Imaging Magnetic Resonance Imaging Quantitative evaluation of light microscopes based on image processing techniques Confocal microscopy of single molecules of the green fluorescent protein Heavy metal contrast enhancement for the selective detection of gold particles in electron microscopical sections using electron spectroscopic imaging
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1