Computational simulation of applying mechanical vibration to mesenchymal stem cell for mechanical modulation toward bone tissue engineering.

Evaluation of cell response to mechanical stimuli at in vitro conditions is known as one of the important issues for modulating cell behavior. Mechanical stimuli, including mechanical vibration and oscillatory fluid flow, act as important biophysical signals for the mechanical modulation of stem cells. In the present study, mesenchymal stem cell (MSC) consists of cytoplasm, nucleus, actin, and microtubule. Also, integrin and primary cilium were considered as mechanoreceptors. In this study, the combined effect of vibration and oscillatory fluid flow on the cell and its components were investigated using numerical modeling. The results of the FEM and FSI model showed that the cell response (stress and strain values) at the frequency of $30Hz$ mechanical vibration has the highest value. The achieved results on shear stress caused by the fluid flow on the cell showed that the cell experiences shear stress in the range of $0.1-10\mathrm{Pa}$. Mechanoreceptors that bind separately to the cell surface, can be highly stimulated by hydrodynamic pressure and, therefore, can play a role in the mechanical modulation of MSCs at in vitro conditions. The results of this research can be effective in future studies to optimize the conditions of mechanical stimuli applied to the cell culture medium and to determine the mechanisms involved in mechanotransduction.