花生蛋白酶解新型抗糖尿病肽的鉴定与体外表征

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-03-01 Epub Date: 2023-11-23 DOI:10.1007/s11130-023-01118-x
Wedad Q Al-Bukhaiti, Sam Al-Dalali, Hanxiang Li, Liyuan Yao, Sherif M Abed, Liyun Zhao, Sheng-Xiang Qiu
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引用次数: 0

摘要

从各种食物中发现的蛋白质中提取的生物活性肽具有显著的健康益处,包括通过抑制碳水化合物水解酶来调节血糖水平。花生蛋白经alcalase (AH)或trypsin (TH)水解后,得到抗糖尿病肽,其抗α-淀粉酶(IC50分别为6.46和5.71 mg/mL)和α-葡萄糖苷酶(IC50分别为6.30和5.57 mg/mL)活性高,抗DPPH•(IC50分别为4.18和3.12 mg/mL)和ABTS•+ (IC50分别为2.87和2.56 mg/mL)活性高。水解产物的生物活性以超滤生成的F3组分最高(50值分别为1.05和0.69 mg/mL), F3- sf8组分对α-淀粉酶和α-葡萄糖苷酶活性的抑制效果最好。采用反相高效液相色谱法对该部位进行纯化,得到活性最高的子组分为F3-SF8-SSF9和SSF10。F3-SF8-SSF9和SSF10肽序列采用LC-MS/MS测定。鉴定出两种具有抑制α-淀粉酶和α-葡萄糖苷酶活性的新型抗糖尿病肽,分别为Asp-Trp-Arg (476.22 Da, IC50分别为0.78和0.35 mg/mL)和ph - tyr (329.15 Da, IC50分别为0.91和0.41 mg/mL)。这些结果表明,从花生蛋白中提取的肽是糖尿病治疗应用的有吸引力的天然成分。
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Identification and in vitro Characterization of Novel Antidiabetic Peptides Released Enzymatically from Peanut Protein.

Bioactive peptides derived from proteins found in various foods provide significant health benefits, including regulating blood sugar levels by inhibiting carbohydrate-hydrolyzing enzymes. Hydrolysates of peanut protein were prepared using alcalase (AH) or trypsin (TH) to generate antidiabetic peptides with high activity against α-amylase (IC50 of 6.46 and 5.71 mg/mL) and α-glucosidase (IC50 of 6.30 and 5.57 mg/mL), as well as antiradical activity to scavenge DPPH (IC50 of 4.18 and 3.12 mg/mL) and ABTS•+ (IC50 of 2.87 and 2.56 mg/mL), respectively. The bioactivities of hydrolysates were greatest in the ultrafiltration-generated F3 fraction (< 3 kDa). The most active fraction was TH-F3, which was purified by gel filtration chromatography to generate sub-fractions (SF). With IC50 values of 1.05 and 0.69 mg/mL, the F3-SF8 fraction was the most effective at inhibiting the activity of α-amylase and α-glucosidase, respectively. This fraction was further purified using RP-HPLC to generate sub-subfractions (SSF), the most active of which were F3-SF8-SSF9 and SSF10. The peptide sequences F3-SF8-SSF9 and SSF10 were determined using LC-MS/MS. Two novel antidiabetic peptides with the potential to inhibit α-amylase and α-glucosidase were identified, with the sequences Asp-Trp-Arg (476.22 Da, IC50 of 0.78, and 0.35 mg/mL) and Phe-Tyr (329.15 Da, IC50 of 0.91, and 0.41 mg/mL). These results suggest that peptides derived from peanut protein are attractive natural ingredients for diabetes management applications.

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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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