α -葡聚糖:一种新型细菌多糖及其作为重金属生物吸附剂的应用。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-11-22 DOI:10.1186/s43141-023-00609-3
Desouky Abd-El-Haleem
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引用次数: 0

摘要

本研究鉴定了一种由velezensis芽孢杆菌菌株40B产生的胞外细菌多糖,其含有超过90%的单糖葡萄糖作为α -葡聚糖。在1074 cm-1处有一个显著的峰,这是糖苷偶联的特征,在FTIR光谱中可见。根据高效液相色谱法的研究,有木糖、蔗糖和乳糖的痕迹。这种细菌葡聚糖作为重金属钴、铬、铜和铅的生物吸附剂,与海藻酸钙珠一起进行了研究。证明了葡聚糖40B对铬离子的亲和力较低,对铅有选择性。初始浓度测量表明,浓度与金属离子消除量呈反比关系。随着葡聚糖用量的增加,铅和铬的去除率增加。结果表明,随着起始溶液pH的升高,金属离子在葡聚糖上的吸附量增加。很明显,随着温度的升高,金属离子的吸附分数略有增加。葡聚糖具有广泛的工业应用,从食品和医药到健康和营养。因此,调查范围扩大到重金属清除。
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Alpha-glucan: a novel bacterial polysaccharide and its application as a biosorbent for heavy metals.

This study identified an extracellular bacterial polysaccharide produced by Bacillus velezensis strain 40B that contains more than 90% of the monosaccharide glucose as alpha-glucan. A prominent peak at 1074 cm-1, a characteristic of glycoside couplings, was visible in the FTIR spectrum. There were traces of xylose, sucrose, and lactose, according to the HPLC study. The ability of this bacterial glucan to operate as a biosorbent of the heavy metals cobalt, chromium, copper, and lead from aqueous solutions was investigated in conjunction with Ca-alginate beads. It proved that glucan 40B has a low affinity for chromium ions and is selective for lead. Initial concentration measurements showed an inverse relationship between concentration and the amount of metal ions eliminated. Lead and chromium removal increased as the glucan dose was increased. It was shown that as the pH of the starting solution is elevated, there is an increase in the sorption of metal ions onto the glucan. It was clear that when the temperature increased, the fraction of metal ion sorption slightly increased. Glucan has a wide range of industrial applications, from food and medicine to health and nutrition. As a result, the investigation's scope was expanded to include heavy metal removal.

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