{"title":"鸡胚提取物DNA亲和纯化蛋白片段中核抗原单克隆抗体的制备。","authors":"S Matsuhashi, Y Arai, T Watanabe, K Hori","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Monoclonal antibodies (Mabs) were raised to components of a DNA binding protein fraction obtained by using native and denatured DNA-cellulose column chromatographies of chick embryo extract. Indirect immunofluorescence microscopic analysis revealed that 3 Mabs, Pr-28, Pr-123 (or Pr-122) and Pr-192 react to nuclear antigens of proliferating cells. Part of the cell nuclei in chick embryo fibroblast culture were stained in speckled patterns by all three of the Mabs. It seems however that they are different clones judging from the following criteria; 1. Pr-28 crossreacts to a P3U1 cell surface antigen but Pr-123 (or Pr-122) and Pr-192 do not. 2. The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation of monoclonal antibodies against nuclear antigens in a DNA affinity purified protein fraction from chick embryo extract.\",\"authors\":\"S Matsuhashi, Y Arai, T Watanabe, K Hori\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Monoclonal antibodies (Mabs) were raised to components of a DNA binding protein fraction obtained by using native and denatured DNA-cellulose column chromatographies of chick embryo extract. Indirect immunofluorescence microscopic analysis revealed that 3 Mabs, Pr-28, Pr-123 (or Pr-122) and Pr-192 react to nuclear antigens of proliferating cells. Part of the cell nuclei in chick embryo fibroblast culture were stained in speckled patterns by all three of the Mabs. It seems however that they are different clones judging from the following criteria; 1. Pr-28 crossreacts to a P3U1 cell surface antigen but Pr-123 (or Pr-122) and Pr-192 do not. 2. The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.</p>\",\"PeriodicalId\":22530,\"journal\":{\"name\":\"The Japanese journal of experimental medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Japanese journal of experimental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Japanese journal of experimental medicine","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation of monoclonal antibodies against nuclear antigens in a DNA affinity purified protein fraction from chick embryo extract.
Monoclonal antibodies (Mabs) were raised to components of a DNA binding protein fraction obtained by using native and denatured DNA-cellulose column chromatographies of chick embryo extract. Indirect immunofluorescence microscopic analysis revealed that 3 Mabs, Pr-28, Pr-123 (or Pr-122) and Pr-192 react to nuclear antigens of proliferating cells. Part of the cell nuclei in chick embryo fibroblast culture were stained in speckled patterns by all three of the Mabs. It seems however that they are different clones judging from the following criteria; 1. Pr-28 crossreacts to a P3U1 cell surface antigen but Pr-123 (or Pr-122) and Pr-192 do not. 2. The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
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