荧光校准器的内在可变性影响了流式细胞术对纳米颗粒和细胞外囊泡的MESF或ERF值的分配。

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2023-11-23 DOI:10.1016/j.nano.2023.102720
Estefanía Lozano-Andrés PhD , Tina Van Den Broeck PhD , Lili Wang PhD , Majid Mehrpouyan PhD , Ye Tian PhD , Xiaomei Yan PhD , Ger J.A. Arkesteijn PhD , Marca H.M. Wauben PhD
{"title":"荧光校准器的内在可变性影响了流式细胞术对纳米颗粒和细胞外囊泡的MESF或ERF值的分配。","authors":"Estefanía Lozano-Andrés PhD ,&nbsp;Tina Van Den Broeck PhD ,&nbsp;Lili Wang PhD ,&nbsp;Majid Mehrpouyan PhD ,&nbsp;Ye Tian PhD ,&nbsp;Xiaomei Yan PhD ,&nbsp;Ger J.A. Arkesteijn PhD ,&nbsp;Marca H.M. Wauben PhD","doi":"10.1016/j.nano.2023.102720","DOIUrl":null,"url":null,"abstract":"<div><p>Flow cytometry allows to characterize nanoparticles (NPs) and extracellular vesicles (EVs) but results are often expressed in arbitrary units of fluorescence. We evaluated the precision and accuracy of molecules of equivalent soluble fluorophores (MESF) beads for calibration of NPs and EVs. Firstly, two FITC-MESF bead sets, 2 and 6 um in size, were measured on three flow cytometers. We showed that arbitrary units could not be compared between instruments but after calibration, comparable FITC MESF units were achieved. However, the two calibration bead sets displayed varying slopes that were consistent across platforms.</p><p>Further investigation revealed that the intrinsic uncertainty related to the MESF beads impacts the robust assignment of values to NPs and EVs based on extrapolation into the dim fluorescence range. Similar variations were found with PE MESF calibration.</p><p>Therefore, the same calibration materials and numbers of calibration points should be used for reliable comparison of submicron sized particles.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"56 ","pages":"Article 102720"},"PeriodicalIF":4.2000,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1549963423000710/pdfft?md5=7ce6e8120251cac93c9d5492d02b8d27&pid=1-s2.0-S1549963423000710-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Intrinsic variability of fluorescence calibrators impacts the assignment of MESF or ERF values to nanoparticles and extracellular vesicles by flow cytometry\",\"authors\":\"Estefanía Lozano-Andrés PhD ,&nbsp;Tina Van Den Broeck PhD ,&nbsp;Lili Wang PhD ,&nbsp;Majid Mehrpouyan PhD ,&nbsp;Ye Tian PhD ,&nbsp;Xiaomei Yan PhD ,&nbsp;Ger J.A. Arkesteijn PhD ,&nbsp;Marca H.M. Wauben PhD\",\"doi\":\"10.1016/j.nano.2023.102720\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Flow cytometry allows to characterize nanoparticles (NPs) and extracellular vesicles (EVs) but results are often expressed in arbitrary units of fluorescence. We evaluated the precision and accuracy of molecules of equivalent soluble fluorophores (MESF) beads for calibration of NPs and EVs. Firstly, two FITC-MESF bead sets, 2 and 6 um in size, were measured on three flow cytometers. We showed that arbitrary units could not be compared between instruments but after calibration, comparable FITC MESF units were achieved. However, the two calibration bead sets displayed varying slopes that were consistent across platforms.</p><p>Further investigation revealed that the intrinsic uncertainty related to the MESF beads impacts the robust assignment of values to NPs and EVs based on extrapolation into the dim fluorescence range. Similar variations were found with PE MESF calibration.</p><p>Therefore, the same calibration materials and numbers of calibration points should be used for reliable comparison of submicron sized particles.</p></div>\",\"PeriodicalId\":19050,\"journal\":{\"name\":\"Nanomedicine : nanotechnology, biology, and medicine\",\"volume\":\"56 \",\"pages\":\"Article 102720\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2023-11-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1549963423000710/pdfft?md5=7ce6e8120251cac93c9d5492d02b8d27&pid=1-s2.0-S1549963423000710-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomedicine : nanotechnology, biology, and medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1549963423000710\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine : nanotechnology, biology, and medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1549963423000710","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

摘要

流式细胞术可以表征纳米颗粒(NPs)和细胞外囊泡(ev),但结果通常以任意单位的荧光表达。我们评估了等效可溶性荧光团(MESF)微球分子的精度和准确性,用于NPs和ev的校准。首先,在三台流式细胞仪上测量两个FITC-MESF头组,大小分别为2 um和6 um。我们发现任意单位不能在仪器之间进行比较,但在校准后,可以获得可比较的FITC MESF单位。然而,两个校准头组显示不同的斜度,在各个平台上是一致的。进一步的研究表明,与MESF珠相关的固有不确定性影响了基于外推到微弱荧光范围的np和ev值的稳健分配。在PE MESF校准中也发现了类似的变化。因此,为了对亚微米级颗粒进行可靠的比较,应使用相同的校准材料和校准点的数量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Intrinsic variability of fluorescence calibrators impacts the assignment of MESF or ERF values to nanoparticles and extracellular vesicles by flow cytometry

Flow cytometry allows to characterize nanoparticles (NPs) and extracellular vesicles (EVs) but results are often expressed in arbitrary units of fluorescence. We evaluated the precision and accuracy of molecules of equivalent soluble fluorophores (MESF) beads for calibration of NPs and EVs. Firstly, two FITC-MESF bead sets, 2 and 6 um in size, were measured on three flow cytometers. We showed that arbitrary units could not be compared between instruments but after calibration, comparable FITC MESF units were achieved. However, the two calibration bead sets displayed varying slopes that were consistent across platforms.

Further investigation revealed that the intrinsic uncertainty related to the MESF beads impacts the robust assignment of values to NPs and EVs based on extrapolation into the dim fluorescence range. Similar variations were found with PE MESF calibration.

Therefore, the same calibration materials and numbers of calibration points should be used for reliable comparison of submicron sized particles.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
期刊最新文献
Comparison of cholesterol transport capacity of peptide- and polymer-based lipid Nanodiscs Retraction notice to “In vitro angiogenic performance and in vivo brain targeting of magnetized endothelial progenitor cells for neurorepair therapies” [Nanomedicine: Nanotechnology, Biology and Medicine 10/1 (2014) 225–234] Facile fabrication of nano-bioactive glass functionalized blended hydrogel with nucleus pulposus-derived MSCs to improve regeneration potential in treatment of disc degeneration by in vivo rat model. Micellar curcumol for maintenance therapy of ovarian cancer by activating the FOXO3a Conceptual rationale for the use of chemically modified nanocomposites for active influence on atherosclerosis using the greater omentum model of experimental animals
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1