精子诱导小鼠卵子中ATP水平的二次增加,这与Ca2+振荡无关。

IF 4.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Journal Pub Date : 2023-12-20 DOI:10.1042/BCJ20230065
Cindy Ikie-Eshalomi, Elnur Aliyev, Sven Hoehn, Tomasz P Jurkowski, Karl Swann
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引用次数: 0

摘要

小鼠卵子受精时的卵子激活是由一系列细胞质Ca2+振荡引起的,该振荡与线粒体活性增加引起的ATP浓度增加有关。我们通过测量小鼠卵中ATP和Ca2+的动态,研究了Ca2+振荡在受精时ATP变化中的作用。初始的ATP增加始于受精时的第一次Ca2+瞬态,然后在大约1小时后发生ATP的二次增加,这先于Ca2+振荡频率的小而暂时的增加。其他刺激引起Ca2+振荡,如PLCz1或硫柳汞,引起ATP的较小或较慢的变化,未能显示出明显的继发性上升。精子诱导的卵子内Ca2+振荡也触发了NADH荧光的变化,这种变化遵循Ca2+峰值的模式,与PLCz1或硫柳汞引发的振荡模式相似。当卵子中含有低浓度的Ca2+螯合剂BAPTA时,精子触发了一个小的Ca2+增加,但仍然有与对照受精卵相似的额外阶段的ATP增加。由thapsigarin引起的单一Ca2+增加在提高ATP水平方面的效果要小得多。综上所述,这些数据表明,在小鼠卵子受精时,次级ATP的增加不是由细胞质Ca2+的增加引起的。受精精子可能通过Ca2+和另一种独立于PLCz1或Ca2+振荡的机制刺激卵子中ATP的产生。
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Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+ oscillations.

Egg activation at fertilization in mouse eggs is caused by a series of cytosolic Ca2+ oscillations that are associated with an increase in ATP concentrations driven by increased mitochondrial activity. We have investigated the role of Ca2+ oscillations in these changes in ATP at fertilization by measuring the dynamics of ATP and Ca2+ in mouse eggs. An initial ATP increase started with the first Ca2+ transient at fertilization and then a secondary increase in ATP occurred ∼1 h later and this preceded a small and temporary increase in the frequency of Ca2+ oscillations. Other stimuli that caused Ca2+ oscillations such as PLCz1 or thimerosal, caused smaller or slower changes in ATP that failed to show the distinct secondary rise. Sperm-induced Ca2+ oscillations in the egg also triggered changes in the fluorescence of NADH which followed the pattern of Ca2+ spikes in a similar pattern to oscillations triggered by PLCz1 or thimerosal. When eggs were loaded with low concentrations of the Ca2+ chelator BAPTA, sperm triggered one small Ca2+ increase, but there were still extra phases of ATP increase that were similar to control fertilized eggs. Singular Ca2+ increases caused by thapsigargin were much less effective in elevating ATP levels. Together these data suggest that the secondary ATP increase at fertilization in mouse eggs is not caused by increases in cytosolic Ca2+. The fertilizing sperm may stimulate ATP production in eggs via both Ca2+ and by another mechanism that is independent of PLCz1 or Ca2+ oscillations.

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来源期刊
Biochemical Journal
Biochemical Journal 生物-生化与分子生物学
CiteScore
8.00
自引率
0.00%
发文量
255
审稿时长
1 months
期刊介绍: Exploring the molecular mechanisms that underpin key biological processes, the Biochemical Journal is a leading bioscience journal publishing high-impact scientific research papers and reviews on the latest advances and new mechanistic concepts in the fields of biochemistry, cellular biosciences and molecular biology. The Journal and its Editorial Board are committed to publishing work that provides a significant advance to current understanding or mechanistic insights; studies that go beyond observational work using in vitro and/or in vivo approaches are welcomed. Painless publishing: All papers undergo a rigorous peer review process; however, the Editorial Board is committed to ensuring that, if revisions are recommended, extra experiments not necessary to the paper will not be asked for. Areas covered in the journal include: Cell biology Chemical biology Energy processes Gene expression and regulation Mechanisms of disease Metabolism Molecular structure and function Plant biology Signalling
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