海洋放线菌的生物多样性及其生物学应用——埃及地中海阿布齐尔湾。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-11-28 DOI:10.1186/s43141-023-00612-8
Ghada E Hegazy, Zakia A Olama, G M Abou-Elela, Heba S Ramadan, Walaa M Ibrahim, Dalia El S El Badan
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引用次数: 0

摘要

背景:放线菌产生次生代谢产物的能力使其成为最重要的原核生物之一。海洋放线菌是用于制药和其他不同行业的最重要的次生代谢物生产者之一。结果:本研究从阿布齐尔湾分离到了有发展前景的放线菌。以硝酸淀粉、淀粉酪蛋白、甘油天冬酰胺和甘油甘氨酸四种不同的培养基作为初步实验培养基,研究了培养基成分对沉积物样品中放线菌数量的影响。结果表明,淀粉酪蛋白培养基在所有试验点中计数最高(30 ~ 63 CFU/g)。在硝酸淀粉和甘油天冬酰胺中检测到较低的计数。另一方面,甘油甘氨酸培养基的计数最低(15-48 CFU/g)。Abu-Qir8的放线菌平均数量最高(63 CFU/g),其次是Abu-Qir1 (48 CFU/g)。Abu-Qir5和Abu-Qir7的计数较低(分别为26和29 CFU/g)。对获得的12株放线菌进行了形态、生理和生化鉴定。对选取的放线菌分离株进行数值分析,大部分分离株可分为A、B、C、D 4个主要聚类,每个聚类含2株;另外,有4个分离株没有聚集在这个相似水平上。分离物W4被选定为最有前途的色素和抗菌剂的生产者;通过统计实验(PBD & BBD)对所得色素进行提取和优化,并对其抗炎活性进行测试。结果表明,在远高于安全剂量的浓度下,BSA蛋白具有抗炎作用,并能阻止其变性,且随色素浓度的增加而增强。结论:海洋放线菌在生产具有多种工业和医药应用价值的新型经济代谢物中起着重要作用。链霉菌属是最重要的放线菌,产生重要的代谢物,如前所述。
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Biodiversity and biological applications of marine actinomycetes-Abu-Qir Bay, Mediterranean Sea, Egypt.

Background: The ability of actinomycetes to produce bioactive secondary metabolites makes them one of the most important prokaryotes. Marine actinomycetes are one of the most important secondary metabolites producers used for pharmaceuticals and other different industries.

Results: In this study, the promising actinomycetes were isolated from Abu-Qir Bay. Four different media named as starch nitrate, starch casein, glycerol asparagine, and glycerol glycine were used as a preliminary experimental media to study the role of the medium components on the counts of actinomycetes in sediment samples. The results indicated that starch casein medium reported the highest counts (30-63 CFU/g) in all the tested sites. Lower counts were detected on starch nitrate and glycerol asparagine. On the other hand, glycerol glycine medium gave the lowest counts (15-48 CFU/g). Abu-Qir8 harbored the highest average count of actinomycetes (63 CFU/g), followed by Abu-Qir1 (48 CFU/g). The lower counts were detected in Abu-Qir5 and Abu-Qir7 (26 and 29 CFU/g, respectively). A total of 12 pure obtained actinomycetes isolates were subjected to morphological, physiological, and biochemical characterization. The selected actinobacterial isolates were subjected to numerical analysis, and the majority of isolates were grouped into four main clusters (A, B, C, & D), and each of them harbored two isolates; additionally, four isolates did not cluster at this similarity level. Isolate W4 was carefully chosen as the most promising pigment and antimicrobial agent's producer; the produced pigment was extracted and optimized by statistical experiments (PBD & BBD) and was tested for its anti-inflammatory activity. The results showed anti-inflammatory effect and prevented the denaturation of BSA protein at a concentration much higher than the safe dose and increased with increasing the pigment concentration.

Conclusion: Marine actinomycetes play a vital role in the production of novel and important economic metabolites that have many industrial and pharmaceuticals applications. Streptomyces genera are the most important actinomycetes that produce important metabolites as previously reported.

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