百合种质的超低温保存:冷冻前后处理分析。

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-09-01
H B Tokgoz, H Karakas, E Kaya, H Yildirim, A F Pirhan, F Altan
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引用次数: 0

摘要

背景:百合(Lilium candidum L.)是一种多年生观赏植物,具有多种药用特性,在化妆品工业中使用。该物种正面临城市化和气候变化的威胁,需要紧急保护。长期保存植物遗传资源最安全、最有效的技术是低温保存,即在极低的温度下保存遗传物质。目的:当今,由于植物自然分布区域的缩小和/或出于不同目的的破坏,植物生物多样性正处于濒危状态。本研究主要是建立一种以白羊草茎尖和愈伤组织为外植体的低温保存方法,以实现白羊草物种的长期保存。材料与方法:L. candidum种群自然分布于土耳其的三个不同地区(Kepsut, Balikesir;艾登八法湖周边地区;和Fethiye-Mugla)离体培养,提供茎尖和愈伤组织外植体。在液滴玻璃化和玻璃化技术冷冻前,用MS营养培养基处理茎尖和愈伤组织,培养基中添加0.4 M蔗糖7 g / L琼脂和植物玻璃化液2 (PVS2)。结果:冷冻保存的茎尖在PVS2处理90分钟后显示出最高的再生水平(71.8%),而愈伤组织在PVS2处理60分钟后显示出最高的再生水平(63.9%)。结论:当使用不同的冷冻保存方法来保存茎尖和愈伤组织外植体时,可以产生高水平的再生。这使得该方法有望长期保存濒危的铁皮酸乳杆菌品种。Doi.org/10.54680/fr23510110512。
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Cryopreservation of Lilium candidum germplasm: analysis of pre- and post-freeze treatments.

Background: Lilium candidum L. is a perennial ornamental plant that has various medicinal properties and is used in the cosmetic industry. The species is facing threats from urbanization and climate change and requires urgent protection. The most secure and efficient technology for the long-term storage of plant genetic resources is cryopreservation, which involves preserving genetic material at extremely low temperatures.

Objective: Today, plant biodiversity is endangered because of the narrowing of its natural distribution areas and/or destruction for different purposes. This study concentrated on creating a cryopreservation process using shoot tips and calluses as explant sources for the long-term conservation of L. candidum species.

Materials and methods: Populations of L. candidum naturally distributed from three different regions of Turkey (Kepsut, Balikesir; the area surrounding Bafa Lake, Aydin; and Fethiye-Mugla) were grown in vitro to supply shoot tip and callus explants. Prior to freezing by droplet-vitrification and vitrification techniques, shoot tips and calluses were treated with MS nutritional medium supplemented with 0.4 M sucrose 7 g per L agar and plant vitrification solution 2 (PVS2).

Results: Cryopreserved shoot tips showed the highest levels of regeneration (71.8%) after a PVS2 treatment of 90 min, while calluses showed the highest levels of regrowth (63.9%) after a PVS2 exposure of 60 min.

Conclusion: High levels of regrowth are produced when the various cryopreservation procedures described here are used to preserve both shoot tip and callus explants. This potentially makes the method promising for the long-term preservation of endangered L. candidum varieties. Doi.org/10.54680/fr23510110512.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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