Forensic application of isotope ratio mass spectrometry (IRMS) for human identification

Application of isotope ratio mass spectrometry (IRMS) to skeletal remains has become an important tool to investigate human behavior and history. Isotopic variations in collagen, enamel, and keratin reflect variations in an individual’s diet and drinking water. Since food and water sources typically are geographically linked, isotope testing can assist in forensic identification by classifying remains to a likely geographic or population origin. If remains are commingled, differences in diet or geographic origin also can support their separation.

The usefulness of IRMS in forensic science is dependent on the underlying quality and surety of the isotope test results; in other words, we need to understand their reliability in interpretations. To take ownership of isotopic data quality, we recommend asking a series of questions:

• 1.

  How well are the mass spectrometer and associated peripheral(s) operating?

• 2.

  Are test results reproducible (over time and between locations)?

• 3.

  Can we differentiate between populations that we would like to?

• 4.

  How variable are the populations of interest?

• 5.

  Have the samples been isotopically altered?

• 6.

  How variable are the samples?

Here, we use data collected during the buildout and accreditation of an isotope testing program at the Defense POW/MIA Accounting Agency (DPAA) to answer the above questions for the forensic application of IRMS for human identification. While our primary focus is on the preparation and analysis of bone collagen, the questions above should be considered whenever isotope testing is used in forensic casework. Whether the populations of interest are drugs or humans, olives or explosives, users need to evaluate their isotopic data and interpretations to ensure they are scientifically sound and legally defensible.