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Vaginal drainage of semen in underwear: A forensic study 内裤中精液的阴道排泄:法医研究
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-14 DOI: 10.1016/j.scijus.2024.09.003
The UK and Ireland Association of Forensic Science Providers’ (AFSP) Body Fluid Forum (BFF) conducted this research to investigate the occurrence of seminal fluid draining from the vagina onto underwear worn after penile-vaginal sexual intercourse with internal ejaculation. This study shows the differing effects of the female being either supine or upright and active on possible patterns of seminal staining on underwear, and the effect of time since intercourse on the deposition of seminal fluid on underwear in a limited study. Here, we demonstrate that post vaginal intercourse different distributions of semen staining can be observed on underwear.
英国和爱尔兰法医科学提供者协会(AFSP)体液论坛(BFF)开展了这项研究,以调查阴茎阴道性交后体内射精时精液从阴道排到所穿内裤上的情况。这项研究显示了女性仰卧或直立活动对内裤上可能出现的精液染色模式的不同影响,并在一项有限的研究中显示了性交后的时间对内裤上精液沉积的影响。在此,我们证明了阴道性交后精液染色在内衣上的不同分布。
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引用次数: 0
The efficacy of Diamond™ nucleic acid dye-stained cell counting techniques for forensic application 用于法医应用的 Diamond™ 核酸染色细胞计数技术的功效
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-08 DOI: 10.1016/j.scijus.2024.09.002

Touch DNA is one of the most common types of biological material collected during criminal investigations. Diamond™ Nucleic Acid Dye (DD) has been shown to aid in touch sample visualisation and target sampling. It has also been used as a method of shedder categorisation that is cheaper and quicker than DNA methods. However, the DD method routinely involves manual cell counting, which can result in intra and inter-person variability similar to other manual techniques used in forensic science, for example, fingerprint identification. Additionally, DD based shedder categorisation involves counting cells in a portion of the touch deposit to extrapolate an individual’s shedder status, and the sampling effect of such estimations is currently unknown.

The present study tested different data analysis aspects of the DD method, including counting variability within and between people, shedder classification differences based on different counting methods (entire thumbprint, sub-section of a print with most cells, sub-section of a print deemed most representative of the entire thumbprint, and random sections), the use of ImageJ software to semi-automate counting and the use and extension of the DD method for investigating DNA Transfer, Persistence, Prevalence and Recovery (DNA-TPPR).

The results of this study show that there are meaningful differences observed during counting processes both between and within people. These differences tended to increase as the factor of time, or the duration of counting, rather than the complexity of cell deposits being assessed. Investment in cell counting software that eliminates personal factors, such as boredom fatigue, can remedy most of these issues, however, will require optimisation, such as fibre recognition. Shedder testing was shown to be affected by the choice of sampling and categorisation methods, and suggested that using an entire finger or larger section size can provide increased precision. Finally, inverted worn gloves stained with DD may provide an acceptable alternative for hands in DNA-TPPR investigations, providing an interesting alternative for future research.

触摸 DNA 是刑事调查中最常见的生物材料之一。钻石™核酸染料 (DD) 已被证明有助于触摸样本的可视化和目标取样。它还被用作脱落物分类方法,比 DNA 方法更便宜、更快捷。不过,DD 方法通常涉及人工细胞计数,这可能会导致人与人之间的差异,类似于法医学中使用的其他人工技术,例如指纹识别。此外,基于脱落细胞计数法的脱落细胞分类涉及对部分触摸沉积物中的细胞进行计数,以推断个体的脱落细胞状态,而这种估计的采样效果目前尚不清楚。本研究测试了 DD 方法的不同数据分析方面,包括人内和人与人之间的计数差异、基于不同计数方法的脱落者分类差异(整个拇指指纹、含有最多细胞的指纹的子部分、被认为最能代表整个拇指指纹的指纹的子部分以及随机部分)、使用 ImageJ 软件进行半自动计数以及使用和扩展 DD 方法调查 DNA 转移、持久性、流行性和恢复(DNA-TPPR)。研究结果表明,在计数过程中,人与人之间以及人与人之间都存在有意义的差异。这些差异往往会随着时间因素或计数持续时间而增加,而不是随着被评估细胞沉积的复杂程度而增加。对细胞计数软件进行投资,消除个人因素(如无聊疲劳),可以解决大部分问题,但需要进行优化,如纤维识别。结果表明,脱落细胞检测受取样和分类方法选择的影响,使用整个手指或更大的切片尺寸可提高精确度。最后,在 DNA-TPPR 调查中,用 DD 染色的反戴式手套可以作为手的替代品,为未来研究提供了一个有趣的选择。
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引用次数: 0
The effects of fingerprinting agents on the stable isotope ratios of polyethylene films 指纹剂对聚乙烯薄膜稳定同位素比率的影响
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-07 DOI: 10.1016/j.scijus.2024.09.001

Traditional forensic chemical comparisons of polymeric materials are often affected by fingerprinting agents that enhance and visualise finger-marks. This can inhibit detailed analysis used to provide an association or discrimination between two samples. In this study, we have demonstrated that the method for polymer comparisons using isotope ratio mass spectrometry analysis is not affected by fingerprinting matter on the surface in contrast to other forensic instrumental tests. A selection of resealable bags was analysed for carbon and hydrogen isotope ratio values of bags that had been subject to various fingerprinting agents and compared to values of untreated bags. The results showed no significant difference between samples that had been fingerprinted from those that were untreated.

对聚合物材料进行传统的法医化学比较时,往往会受到指纹印剂的影响,因为指纹印剂会增强指纹痕迹并使其可视化。这可能会妨碍用于关联或区分两种样品的详细分析。在本研究中,我们证明了利用同位素比质谱分析进行聚合物比较的方法不会受到表面指纹物质的影响,这一点与其他法医仪器测试不同。我们对一些可重新密封的袋子进行了分析,以确定经过各种指纹鉴定剂处理的袋子的碳和氢同位素比值,并与未经处理的袋子的比值进行比较。结果表明,打过指纹的样本与未打指纹的样本没有明显差别。
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引用次数: 0
BM1: Events Guide BM1:活动指南
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-01 DOI: 10.1016/S1355-0306(24)00088-1
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引用次数: 0
Letter to the editor: “Edwards curve digital signature algorithm for video integrity verification on blockchain framework” 致编辑的信:"区块链框架上用于视频完整性验证的爱德华曲线数字签名算法"
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-01 DOI: 10.1016/j.scijus.2024.08.005

Video integrity is a crucial aspect of forensic science that guarantees the reliability and validity of visual evidence used in court proceedings. In an era where digital alteration tools are readily available, ensuring that video recordings remain unmodified is essential to upholding the integrity of the legal system. Our technique offers a new, simple way to check the integrity of video data. Our approach makes use of the BLAKE2b hash function, the blockchain, and the Edwards Curve Digital Signature Algorithm. Video segments are pre-recorded video clips for which signatures are generated and kept in chronological blocks. To provide an additional degree of protection, the signature from the prior block is kept in the present block. These signatures are validated at the moment of validation. According to experimental data, our method performs faster and more securely than state-of-the-art approaches. With negligible extra storage requirements, our approach can detect every kind of counterfeit on any video file, by anybody, at any time. Our security analysis further demonstrates that our approach is resistant to a wide range of attacks, such as side channel, collision, key substitution, and chosen message assaults.

视频完整性是法医学的一个重要方面,它保证了法庭诉讼中使用的视觉证据的可靠性和有效性。在数字篡改工具唾手可得的时代,确保视频记录不被篡改对于维护法律体系的完整性至关重要。我们的技术为检查视频数据的完整性提供了一种简单的新方法。我们的方法利用了 BLAKE2b 哈希函数、区块链和爱德华兹曲线数字签名算法。视频段是预先录制的视频片段,为其生成签名并按时间顺序保存在区块中。为了提供额外的保护,前一个区块的签名保存在当前区块中。这些签名在验证时进行验证。根据实验数据,我们的方法比最先进的方法更快、更安全。我们的方法所需的额外存储空间几乎可以忽略不计,可以随时检测到任何人对任何视频文件的各种伪造行为。我们的安全分析进一步证明,我们的方法可以抵御各种攻击,如侧信道攻击、碰撞攻击、密钥替换攻击和选择信息攻击。
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引用次数: 0
The impact of accelerant facilitated fire on blood detection and the efficacy of subsequent soot removal methods 助燃剂助燃对血迹检测的影响以及后续烟尘清除方法的功效
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-01 DOI: 10.1016/j.scijus.2024.08.007

Previous literature has established that recovering heat damaged body fluids is possible, however with little investigation into the effect of accelerants used in initiating arson fires. This study therefore aimed to determine whether presumptive blood detection was affected by heat damage resulting from accelerant facilitated fires. Another objective was to examine various techniques for removing soot, which is a noted barrier to blood detection. The study focused on blood deposited on household flooring materials, one porous and one nonporous surface: carpet and tile respectively. Samples were burned with butane, petrol, and kerosene then presumptively tested using the Kastle Meyer colourimetric blood detection test. Testing was then repeated following soot removal by either wiping, scraping, or using liquid latex. The “strength” of positive detections was evaluated using a scale based on reaction speed and colour intensity. Results demonstrated that accelerants weakened detection strength, although nearly all samples tested positive overall, and the impact of each accelerant on both surface types was largely similar. It was also discovered that soot removal improved the strength of blood detection results in approximately 69% of carpet and 47% of tile samples, with wiping being the superior method on both surface types. Consequently, introducing this investigative step may be critical to maximizing blood evidence recovery in arson casework. These findings indicate the worth in recovering severely burned items, particularly for evidence as crucial as blood.

以往的文献已经证实,恢复受热损伤的体液是可能的,但对引发纵火的助燃剂的影响却鲜有调查。因此,本研究旨在确定推定血液检测是否会受到助燃剂引发的火灾所造成的热损伤的影响。另一个目的是研究清除烟灰的各种技术,烟灰是血液检测的一个显著障碍。研究的重点是沉积在家用地板材料上的血液,一种是多孔表面,一种是无孔表面:分别是地毯和瓷砖。样本用丁烷、汽油和煤油焚烧,然后使用 Kastle Meyer 比色血液检测测试法进行推定检测。然后通过擦拭、刮擦或使用液态乳胶清除烟灰后重复检测。阳性检测的 "强度 "是根据反应速度和颜色强度来评估的。结果表明,尽管几乎所有样品的检测结果都呈阳性,但促进剂会削弱检测强度,而且每种促进剂对两种表面类型的影响基本相似。研究还发现,在约 69% 的地毯样本和 47% 的瓷砖样本中,清除烟灰可提高血液检测结果的强度,而在这两种表面类型中,擦拭是更优越的方法。因此,在纵火案中引入这一调查步骤可能对最大限度地恢复血迹证据至关重要。这些发现表明,恢复被严重烧毁的物品,尤其是像血液这样至关重要的证据,是非常有价值的。
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引用次数: 0
BM2: Council Information BM2:理事会信息
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-01 DOI: 10.1016/S1355-0306(24)00089-3
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引用次数: 0
Prelim 3: Contents 预赛 3:内容
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-01 DOI: 10.1016/S1355-0306(24)00087-X
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引用次数: 0
Letter to the editor: “Edwards curve digital signature algorithm for video integrity verification on blockchain framework” 致编辑的信:"区块链框架上用于视频完整性验证的爱德华曲线数字签名算法"
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-09-01 DOI: 10.1016/j.scijus.2024.08.004
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引用次数: 0
A preliminary study on the stability of bare footprint linear measurements in four motion states 关于四种运动状态下裸脚印线性测量稳定性的初步研究
IF 1.9 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2024-08-13 DOI: 10.1016/j.scijus.2024.08.006

In some crime scenes, there may be bare footprints. Analyzing and testing the linear measurements of bare footprints in crime scenes can play an important role in personal analysis and individual identification. However, the linear measurements of bare footprints may be influenced by different motion states, leading to changes in length and width or even significant deviations. Previous studies focused on the linear differences between static and dynamic footprints, and failed to take the speed factor into consideration. This paper studied the stability and change regularities of the linear measurements of bare footprints in four different motion states: standing, normal walking, fast walking and trotting. Dust footprints of the right feet were collected from 80 healthy young adults under these four motion states. Seven linear measurements were obtained for each footprint using the Reel method, totaling 2240 data sets. One-way repeated measures ANOVA was used to assess the measurement variations across the four states. The results showed that there were statistically significant variances in the length measurements (A1-A5) between the standing state and other motion states, whereas no statistically significant variances were observed between the three dynamic states. It was found that the mean values of the five length measurements (A1–A5) increased from static to dynamic state, and then gradually decreased slightly as the walking speed increased. Additionally, no significant differences were found in the two width measurements (MPJ Width and Calc Width) between the four motion states. As a preliminary study, this study can provide a reference for the analysis of bare footprints in different motion states extracted from crime scenes.

在某些犯罪现场,可能会出现裸脚印。分析和测试犯罪现场裸脚印的线性测量值可在个人分析和个人识别中发挥重要作用。然而,裸脚印的线性测量值可能会受到不同运动状态的影响,导致长度和宽度发生变化,甚至出现明显偏差。以往的研究主要关注静态脚印和动态脚印的线性差异,没有考虑速度因素。本文研究了裸足在站立、正常行走、快速行走和小跑四种不同运动状态下线性测量结果的稳定性和变化规律。本文采集了 80 名健康青壮年在这四种运动状态下的右脚灰尘脚印。采用里尔法对每个脚印进行了七次线性测量,共获得 2240 组数据。采用单因子重复测量方差分析来评估四种状态下的测量差异。结果表明,站立状态与其他运动状态之间的长度测量值(A1-A5)存在显著的统计学差异,而三种动态状态之间则没有显著的统计学差异。研究发现,从静态到动态,五项长度测量值(A1-A5)的平均值均有所增加,然后随着步行速度的增加而逐渐略有减少。此外,两种宽度测量值(MPJ 宽度和 Calc 宽度)在四种运动状态之间没有发现明显差异。作为一项初步研究,本研究可为分析从犯罪现场提取的不同运动状态下的裸脚印提供参考。
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引用次数: 0
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Science & Justice
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