Yan Ding, Shulei Zhang, Xiuhui Zang, Mengli Ding, Caifeng Ding
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Electrochemical signal molecules, ferrocenecarboxylic acid (Fc) and methylene blue (MB), were modified to another end of multifunctional peptide and interior of MXene-Au@ZIF-67, respectively, to produce a ratiometric electrochemical signal. We selected prostate specific antigen (PSA) as the model compound. PSA specifically recognizes and cleaves the recognition segment in the Y-shaped peptide, and the signal of Fc is reduced, while the signal of MB remains unchanged. The ratiometric strategy endows the present biosensor high accuracy and sensitivity with a detection limit of 0.85 pg/mL. In addition, the sensing surface has good antifouling ability due to the antifouling sequence of the two branching parts of the Y-shaped peptide. 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引用次数: 0
摘要
基于所设计的倒 "Y "形肽和MXene纳米复合材料(MXene-Au@ZIF-67),设计了一种比率计抗污染电化学生物传感器,并将其应用于血清中生物标志物的检测。插入 MXene 内部的 Au@ZIF-67 不仅能防止 MXene 的积累,还能为捕获生物大分子提供大量的结合位点。设计的多功能 Y 型肽包含锚定、防污和识别序列,通过 Au-S 键锚定在 MXene-Au@ZIF-67 上。电化学信号分子二茂铁羧酸(Fc)和亚甲基蓝(MB)分别修饰在多功能肽的另一端和 MXene-Au@ZIF-67 的内部,以产生比率电化学信号。我们选择前列腺特异性抗原(PSA)作为模型化合物。PSA 能特异性地识别并裂解 Y 型肽中的识别段,Fc 信号减弱,而 MB 信号保持不变。这种比率测量策略使本生物传感器具有很高的准确度和灵敏度,检测限为 0.85 pg/mL。此外,由于 Y 型肽的两个分支部分具有防污序列,因此传感表面具有良好的防污能力。更重要的是,通过替换肽的识别段,还可以检测其他目标,这表明通用检测策略有望应用于临床诊断中各种生物标记物的检测。
Ratiometric antifouling electrochemical biosensors based on designed Y-shaped peptide and MXene loaded with Au@ZIF-67 and methylene blue
Based on the designed inverted Y-shaped peptide and MXene nanocomposite (MXene-Au@ZIF-67), a ratiometric anti-pollution electrochemical biosensor was designed and applied to the detection of biomarkers in serum. Au@ZIF-67 inserted into the interior of MXene can not only prevent the accumulation of MXene but also provide a large amounts of binding sites for capturing biomolecules. A designed multifunctional Y-shaped peptide containing anchoring, antifouling, and recognition sequences was anchored onto MXene-Au@ZIF-67 through Au–S bonds. Electrochemical signal molecules, ferrocenecarboxylic acid (Fc) and methylene blue (MB), were modified to another end of multifunctional peptide and interior of MXene-Au@ZIF-67, respectively, to produce a ratiometric electrochemical signal. We selected prostate specific antigen (PSA) as the model compound. PSA specifically recognizes and cleaves the recognition segment in the Y-shaped peptide, and the signal of Fc is reduced, while the signal of MB remains unchanged. The ratiometric strategy endows the present biosensor high accuracy and sensitivity with a detection limit of 0.85 pg/mL. In addition, the sensing surface has good antifouling ability due to the antifouling sequence of the two branching parts of the Y-shaped peptide. More importantly, by replacing the recognition segment of peptides also other targets are accessible, indicating the potential application of the universal detection strategy to the detection of various biomarkers in clinical diagnosis.
期刊介绍:
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.