在农杆菌中,抗生素和群体感应调节剂的AccA转运体的高度保守的配体结合位点导致了不同的特异性。

IF 4.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Journal Pub Date : 2024-01-25 DOI:10.1042/BCJ20230273
Solange Moréra, Armelle Vigouroux, Magali Aumont-Nicaise, Mohammed Ahmar, Thibault Meyer, Abbas El Sahili, Grégory Deicsics, Almudena González-Mula, Sizhe Li, Jeanne Doré, Serena Sirigu, Pierre Legrand, Camille Penot, François André, Denis Faure, Laurent Soulère, Yves Queneau, Ludovic Vial
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引用次数: 0

摘要

经致病性农杆菌菌株C58基因修饰的植物合成agrocinopines A和B,而经致病性菌株Bo542基因修饰的植物产生agrocinopines C和D。这四种agrocinopines (A、B、C和D)通过农杆菌和信号分子作为营养物质传播毒力基因。它们共享罕见的吡喃糖-2-磷酸基序,以agrocinopines A/B中的L-arabinopyranose片段和agrocinopines C/D中的D-glucopyranose片段为代表,也在抗生素agrocin 84中发现。它们通过Acc转运系统(包括与ABC转运蛋白偶联的溶质结合蛋白AccA)进入农杆菌细胞质。我们之前意外地发现,来自菌株C58 (AccAC58)的AccA识别了所有四种agrocinopines和agrocin84中存在的pyranose-2-phosphate基序,这意味着菌株C58能够进口源自竞争菌株Bo542的agrocinopines C/D。在这里,我们使用农用罂粟碱衍生物,结合晶体学、亲和力和稳定性测量、建模、分子动力学、体外和体内实验,我们发现AccABo542和AccAC58在75%的序列相同和几乎相同的配体结合位点的情况下表现不同。事实上,菌株Bo542只输入含有d -葡萄糖吡喃糖-2-磷酸片段的化合物,与菌株C58相比,亲和力较低。这种进口效率的差异使得C58在培养基上比Bo542更具竞争力。我们现在可以解释为什么农杆菌/葡萄异根菌菌株S4对agrocin 84不敏感,尽管它的基因组包含一个保守的Acc转运系统。总的来说,我们的工作突出了AccA蛋白作为一个案例研究,其稳定性和动力学驱动特异性。
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A highly conserved ligand-binding site for AccA transporters of antibiotic and quorum-sensing regulator in Agrobacterium leads to a different specificity.

Plants genetically modified by the pathogenic Agrobacterium strain C58 synthesize agrocinopines A and B, whereas those modified by the pathogenic strain Bo542 produce agrocinopines C and D. The four agrocinopines (A, B, C and D) serve as nutrients by agrobacteria and signaling molecule for the dissemination of virulence genes. They share the uncommon pyranose-2-phosphate motif, represented by the l-arabinopyranose moiety in agrocinopines A/B and the d-glucopyranose moiety in agrocinopines C/D, also found in the antibiotic agrocin 84. They are imported into agrobacterial cytoplasm via the Acc transport system, including the solute-binding protein AccA coupled to an ABC transporter. We have previously shown that unexpectedly, AccA from strain C58 (AccAC58) recognizes the pyranose-2-phosphate motif present in all four agrocinopines and agrocin 84, meaning that strain C58 is able to import agrocinopines C/D, originating from the competitor strain Bo542. Here, using agrocinopine derivatives and combining crystallography, affinity and stability measurements, modeling, molecular dynamics, in vitro and vivo assays, we show that AccABo542 and AccAC58 behave differently despite 75% sequence identity and a nearly identical ligand binding site. Indeed, strain Bo542 imports only compounds containing the d-glucopyranose-2-phosphate moiety, and with a lower affinity compared with strain C58. This difference in import efficiency makes C58 more competitive than Bo542 in culture media. We can now explain why Agrobacterium/Allorhizobium vitis strain S4 is insensitive to agrocin 84, although its genome contains a conserved Acc transport system. Overall, our work highlights AccA proteins as a case study, for which stability and dynamics drive specificity.

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来源期刊
Biochemical Journal
Biochemical Journal 生物-生化与分子生物学
CiteScore
8.00
自引率
0.00%
发文量
255
审稿时长
1 months
期刊介绍: Exploring the molecular mechanisms that underpin key biological processes, the Biochemical Journal is a leading bioscience journal publishing high-impact scientific research papers and reviews on the latest advances and new mechanistic concepts in the fields of biochemistry, cellular biosciences and molecular biology. The Journal and its Editorial Board are committed to publishing work that provides a significant advance to current understanding or mechanistic insights; studies that go beyond observational work using in vitro and/or in vivo approaches are welcomed. Painless publishing: All papers undergo a rigorous peer review process; however, the Editorial Board is committed to ensuring that, if revisions are recommended, extra experiments not necessary to the paper will not be asked for. Areas covered in the journal include: Cell biology Chemical biology Energy processes Gene expression and regulation Mechanisms of disease Metabolism Molecular structure and function Plant biology Signalling
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