利用电荷耦合器件光学成像和长通滤光片对放射性核素衍生切伦科夫辐射引起的光敏剂发射进行成像评估。

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING World journal of radiology Pub Date : 2023-11-28 DOI:10.4329/wjr.v15.i11.315
Winn Aung, Atsushi B Tsuji, Kazuaki Rikiyama, Fumihiko Nishikido, Satoshi Obara, Tatsuya Higashi
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引用次数: 0

摘要

背景:放射性核素产生切伦科夫辐射(Cherenkov radiation, CR),在光疗中可能激活光敏剂(PSs)。几个研究小组利用光学成像研究了切伦科夫能量转移到ps;然而,经济有效地确定放射性核素衍生的CR是否激发ps并检测激发ps的荧光发射仍然是一个挑战。许多实验室都需要昂贵的专用设备。目的:经济有效地确认核素衍生的CR是否激发了ps,并区分激发后的ps的荧光发射。方法:采用微孔板阅读器和荧光光谱仪对ps的吸光度和荧光光谱进行测定,检测ps的光物理性质。为了减少对昂贵的专用设备的需求并实现研究的目的,我们开发了一种利用电荷耦合器件光学成像系统和适当的不同波长的长通滤波器的方法(手动顺序应用515、580、645、700、750和800 nm的长通滤波器)。以四(4-羧基苯基)卟啉(TCPP)作为模型PS,以不同剂量的铜-64 (64CuCl2)(4,2和1 mCi)作为产cr的放射性核素。样品制备后0.5 h进行成像和数据采集。采用ImageJ软件(美国国立卫生研究院)进行差分图像分析,直观评价TCPP荧光。结果:TCPP的最大吸光度在390 ~ 430 nm处,发射峰在670 nm处。利用光学成像系统和上述高透射率长通滤光片观察了CR和CR诱导的TCPP发射。根据64CuCl2 + TCPP与64CuCl2的串行信号强度(总通量)差值进行计算和相减,得到645 ~ 700 nm窗口内峰值的TCPP发射光谱。此外,在64CuCl2 + TCPP图像中减去64CuCl2图像,得到TCPP的差异荧光图像。考虑不同64CuCl2剂量的实验结果呈现剂量依赖趋势。这些结果表明,结合不同长通滤波器和减法图像处理的生物发光成像装置可以确定cr诱导的TCPP的发射光谱和差分荧光图像。结论:该方法可识别放射性核素衍生CR产生的PS荧光发射,有助于加速Cherenkov能量转移成像技术的发展和新PS的发现。
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Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters.

Background: Radionuclides produce Cherenkov radiation (CR), which can potentially activate photosensitizers (PSs) in phototherapy. Several groups have studied Cherenkov energy transfer to PSs using optical imaging; however, cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge. Many laboratories face the need for expensive dedicated equipment.

Aim: To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.

Methods: The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs. To mitigate the need for expensive dedicated equipment and achieve the aim of the study, we developed a method that utilizes a charge-coupled device optical imaging system and appropriate long-pass filters of different wavelengths (manual sequential application of long-pass filters of 515, 580, 645, 700, 750, and 800 nm). Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was utilized as a model PS. Different doses of copper-64 (64CuCl2) (4, 2, and 1 mCi) were used as CR-producing radionuclides. Imaging and data acquisition were performed 0.5 h after sample preparation. Differential image analysis was conducted by using ImageJ software (National Institutes of Health) to visually evaluate TCPP fluorescence.

Results: The maximum absorbance of TCPP was at 390-430 nm, and the emission peak was at 670 nm. The CR and CR-induced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above. The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity (total flux) difference between 64CuCl2 + TCPP and 64CuCl2. Moreover, the differential fluorescence images of TCPP were obtained by subtracting the 64CuCl2 image from the 64CuCl2 + TCPP image. The experimental results considering different 64CuCl2 doses showed a dose-dependent trend. These results demonstrate that a bioluminescence imaging device coupled with different long-pass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.

Conclusion: This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.

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来源期刊
World journal of radiology
World journal of radiology RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
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