利用电荷耦合器件光学成像和长通滤光片对放射性核素衍生切伦科夫辐射引起的光敏剂发射进行成像评估。

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING World journal of radiology Pub Date : 2023-11-28 DOI:10.4329/wjr.v15.i11.315
Winn Aung, Atsushi B Tsuji, Kazuaki Rikiyama, Fumihiko Nishikido, Satoshi Obara, Tatsuya Higashi
{"title":"利用电荷耦合器件光学成像和长通滤光片对放射性核素衍生切伦科夫辐射引起的光敏剂发射进行成像评估。","authors":"Winn Aung, Atsushi B Tsuji, Kazuaki Rikiyama, Fumihiko Nishikido, Satoshi Obara, Tatsuya Higashi","doi":"10.4329/wjr.v15.i11.315","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Radionuclides produce Cherenkov radiation (CR), which can potentially activate photosensitizers (PSs) in phototherapy. Several groups have studied Cherenkov energy transfer to PSs using optical imaging; however, cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge. Many laboratories face the need for expensive dedicated equipment.</p><p><strong>Aim: </strong>To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.</p><p><strong>Methods: </strong>The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs. To mitigate the need for expensive dedicated equipment and achieve the aim of the study, we developed a method that utilizes a charge-coupled device optical imaging system and appropriate long-pass filters of different wavelengths (manual sequential application of long-pass filters of 515, 580, 645, 700, 750, and 800 nm). Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was utilized as a model PS. Different doses of copper-64 (<sup>64</sup>CuCl<sub>2</sub>) (4, 2, and 1 mCi) were used as CR-producing radionuclides. Imaging and data acquisition were performed 0.5 h after sample preparation. Differential image analysis was conducted by using ImageJ software (National Institutes of Health) to visually evaluate TCPP fluorescence.</p><p><strong>Results: </strong>The maximum absorbance of TCPP was at 390-430 nm, and the emission peak was at 670 nm. The CR and CR-induced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above. The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity (total flux) difference between <sup>64</sup>CuCl<sub>2</sub> + TCPP and <sup>64</sup>CuCl<sub>2</sub>. Moreover, the differential fluorescence images of TCPP were obtained by subtracting the <sup>64</sup>CuCl<sub>2</sub> image from the <sup>64</sup>CuCl<sub>2</sub> + TCPP image. The experimental results considering different <sup>64</sup>CuCl<sub>2</sub> doses showed a dose-dependent trend. These results demonstrate that a bioluminescence imaging device coupled with different long-pass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.</p><p><strong>Conclusion: </strong>This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.</p>","PeriodicalId":23819,"journal":{"name":"World journal of radiology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696188/pdf/","citationCount":"0","resultStr":"{\"title\":\"Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters.\",\"authors\":\"Winn Aung, Atsushi B Tsuji, Kazuaki Rikiyama, Fumihiko Nishikido, Satoshi Obara, Tatsuya Higashi\",\"doi\":\"10.4329/wjr.v15.i11.315\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Radionuclides produce Cherenkov radiation (CR), which can potentially activate photosensitizers (PSs) in phototherapy. Several groups have studied Cherenkov energy transfer to PSs using optical imaging; however, cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge. Many laboratories face the need for expensive dedicated equipment.</p><p><strong>Aim: </strong>To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.</p><p><strong>Methods: </strong>The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs. To mitigate the need for expensive dedicated equipment and achieve the aim of the study, we developed a method that utilizes a charge-coupled device optical imaging system and appropriate long-pass filters of different wavelengths (manual sequential application of long-pass filters of 515, 580, 645, 700, 750, and 800 nm). Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was utilized as a model PS. Different doses of copper-64 (<sup>64</sup>CuCl<sub>2</sub>) (4, 2, and 1 mCi) were used as CR-producing radionuclides. Imaging and data acquisition were performed 0.5 h after sample preparation. Differential image analysis was conducted by using ImageJ software (National Institutes of Health) to visually evaluate TCPP fluorescence.</p><p><strong>Results: </strong>The maximum absorbance of TCPP was at 390-430 nm, and the emission peak was at 670 nm. The CR and CR-induced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above. The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity (total flux) difference between <sup>64</sup>CuCl<sub>2</sub> + TCPP and <sup>64</sup>CuCl<sub>2</sub>. Moreover, the differential fluorescence images of TCPP were obtained by subtracting the <sup>64</sup>CuCl<sub>2</sub> image from the <sup>64</sup>CuCl<sub>2</sub> + TCPP image. The experimental results considering different <sup>64</sup>CuCl<sub>2</sub> doses showed a dose-dependent trend. These results demonstrate that a bioluminescence imaging device coupled with different long-pass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.</p><p><strong>Conclusion: </strong>This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.</p>\",\"PeriodicalId\":23819,\"journal\":{\"name\":\"World journal of radiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696188/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"World journal of radiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4329/wjr.v15.i11.315\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of radiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4329/wjr.v15.i11.315","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
引用次数: 0

摘要

背景:放射性核素产生切伦科夫辐射(Cherenkov radiation, CR),在光疗中可能激活光敏剂(PSs)。几个研究小组利用光学成像研究了切伦科夫能量转移到ps;然而,经济有效地确定放射性核素衍生的CR是否激发ps并检测激发ps的荧光发射仍然是一个挑战。许多实验室都需要昂贵的专用设备。目的:经济有效地确认核素衍生的CR是否激发了ps,并区分激发后的ps的荧光发射。方法:采用微孔板阅读器和荧光光谱仪对ps的吸光度和荧光光谱进行测定,检测ps的光物理性质。为了减少对昂贵的专用设备的需求并实现研究的目的,我们开发了一种利用电荷耦合器件光学成像系统和适当的不同波长的长通滤波器的方法(手动顺序应用515、580、645、700、750和800 nm的长通滤波器)。以四(4-羧基苯基)卟啉(TCPP)作为模型PS,以不同剂量的铜-64 (64CuCl2)(4,2和1 mCi)作为产cr的放射性核素。样品制备后0.5 h进行成像和数据采集。采用ImageJ软件(美国国立卫生研究院)进行差分图像分析,直观评价TCPP荧光。结果:TCPP的最大吸光度在390 ~ 430 nm处,发射峰在670 nm处。利用光学成像系统和上述高透射率长通滤光片观察了CR和CR诱导的TCPP发射。根据64CuCl2 + TCPP与64CuCl2的串行信号强度(总通量)差值进行计算和相减,得到645 ~ 700 nm窗口内峰值的TCPP发射光谱。此外,在64CuCl2 + TCPP图像中减去64CuCl2图像,得到TCPP的差异荧光图像。考虑不同64CuCl2剂量的实验结果呈现剂量依赖趋势。这些结果表明,结合不同长通滤波器和减法图像处理的生物发光成像装置可以确定cr诱导的TCPP的发射光谱和差分荧光图像。结论:该方法可识别放射性核素衍生CR产生的PS荧光发射,有助于加速Cherenkov能量转移成像技术的发展和新PS的发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters.

Background: Radionuclides produce Cherenkov radiation (CR), which can potentially activate photosensitizers (PSs) in phototherapy. Several groups have studied Cherenkov energy transfer to PSs using optical imaging; however, cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge. Many laboratories face the need for expensive dedicated equipment.

Aim: To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.

Methods: The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs. To mitigate the need for expensive dedicated equipment and achieve the aim of the study, we developed a method that utilizes a charge-coupled device optical imaging system and appropriate long-pass filters of different wavelengths (manual sequential application of long-pass filters of 515, 580, 645, 700, 750, and 800 nm). Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was utilized as a model PS. Different doses of copper-64 (64CuCl2) (4, 2, and 1 mCi) were used as CR-producing radionuclides. Imaging and data acquisition were performed 0.5 h after sample preparation. Differential image analysis was conducted by using ImageJ software (National Institutes of Health) to visually evaluate TCPP fluorescence.

Results: The maximum absorbance of TCPP was at 390-430 nm, and the emission peak was at 670 nm. The CR and CR-induced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above. The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity (total flux) difference between 64CuCl2 + TCPP and 64CuCl2. Moreover, the differential fluorescence images of TCPP were obtained by subtracting the 64CuCl2 image from the 64CuCl2 + TCPP image. The experimental results considering different 64CuCl2 doses showed a dose-dependent trend. These results demonstrate that a bioluminescence imaging device coupled with different long-pass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.

Conclusion: This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
World journal of radiology
World journal of radiology RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
自引率
8.00%
发文量
35
期刊最新文献
Asymmetric outcomes in bilateral maxillary impacted tooth extractions: A case report. Breast cancer imaging-clinical experience with two-dimensional-shear wave elastography: A retrospective study. Cryoablation for intrapulmonary bronchial cyst: A case report. Cystic ductal adenocarcinoma of pancreas complicated with neuroendocrine tumor: A case report and review of literature. Ectopic recurrence following treatment of arteriovenous malformations in an adult: A case report and review of literature.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1