Rapid and sensitive screening system of Mycoplasma hyorhinis based on Sandwich-DNA hybridization with nano-amplifiers

Mycoplasma contamination in cell/tissue cultures significantly affects cell characteristics, susceptibility to infectious pathogens, and drug reactions without obvious morphological changes and noticeable changes in cell growth rates. Although the technology for screening mycoplasma is essential, conventional technologies have limitations in the selection of a quick and easy manner. Here, we introduce an innovative screening system for mycoplasma based on electrochemical methods. In the electrochemical screening, a newly designed electrode, consisting of a sandwich-DNA hybridization for high selectivity and a nano-amplifier to improve the sensitivity, has implemented a realistic screening system by presenting detection capability at extremely low (ng/μL) levels and detection potential in real samples. Therefore, this system offers rapid screening, data accuracy, temporal effectiveness, and affordable-and-portable installation, enabling timely elimination of Mycoplasma contamination and facilitating progress in biomedical and pharmaceutical research.