Yan Zhou, Lulu Hao, Lexiang Huang, Xiaoming Tang, Danting Zhuo, Li Yun Wang, Ying Zhang
{"title":"从头转录组组装揭示了花性决定的特征","authors":"Yan Zhou, Lulu Hao, Lexiang Huang, Xiaoming Tang, Danting Zhuo, Li Yun Wang, Ying Zhang","doi":"10.1186/s13595-022-01156-6","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Key message</h3><p>MADS-box genes family may play important roles in the flower sex determination in <i>Excoecaria agallocha</i>. A total of 73 MADS-box genes were identified in <i>E. agallocha</i>. De novo transcriptome assembly analysis suggested that <i>AP1/FUL</i>, <i>AP3/PI</i>, <i>AGL104</i>, and <i>SOC1</i> plays potential roles in <i>E. agallocha</i> flower sex determination.</p><h3 data-test=\"abstract-sub-heading\">Context</h3><p><i>Excoecaria agallocha</i> is a dioecious species containing both male and female individuals producing unisexual flowers. The underlying mechanisms regulating inflorescence differentiation in these species remains poorly understood.</p><h3 data-test=\"abstract-sub-heading\">Aims</h3><p>Flower development influences reproduction and breeding in <i>E.agallocha</i>, which contributes to ecological restoration in the intertidal zone.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>We performed de novo transcriptome assembly analysis on male and female flowers and leaves from <i>E. agallocha</i>.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>We identified a total of 73 MADS-box genes in the <i>E. agallocha</i> genome, which we grouped into five distinct classes (MIKC<sup>c</sup>, Mα, Mβ, Mγ, MIKC*) after phylogenetic comparisons with <i>J. curcas</i> homologs. We analyzed expression profiles and suggested <i>AP1/FUL</i>, <i>AP3/PI</i>, <i>AGL104</i>, and <i>SOC1</i> as candidate regulators of sex determination. In addition, several <i>EaMADS</i> genes were significantly upregulated in flowers compared to leaves.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>Our study represents the first detailed, comprehensive evaluation of the regulation of MADS-box genes associated with sex determination in <i>E. agallocha</i>. The assembled transcriptomic profiles increase the genetic information available for this species and constitute an important contribution to functional studies of inflorescence genes. In turn, this will help exploring the molecular mechanisms underlying the evolution of <i>E. agallocha</i>.</p>","PeriodicalId":7994,"journal":{"name":"Annals of Forest Science","volume":"11 6","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"De novo transcriptome assembly reveals characteristics of flower sex determination of Excoecaria agallocha\",\"authors\":\"Yan Zhou, Lulu Hao, Lexiang Huang, Xiaoming Tang, Danting Zhuo, Li Yun Wang, Ying Zhang\",\"doi\":\"10.1186/s13595-022-01156-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Key message</h3><p>MADS-box genes family may play important roles in the flower sex determination in <i>Excoecaria agallocha</i>. A total of 73 MADS-box genes were identified in <i>E. agallocha</i>. De novo transcriptome assembly analysis suggested that <i>AP1/FUL</i>, <i>AP3/PI</i>, <i>AGL104</i>, and <i>SOC1</i> plays potential roles in <i>E. agallocha</i> flower sex determination.</p><h3 data-test=\\\"abstract-sub-heading\\\">Context</h3><p><i>Excoecaria agallocha</i> is a dioecious species containing both male and female individuals producing unisexual flowers. The underlying mechanisms regulating inflorescence differentiation in these species remains poorly understood.</p><h3 data-test=\\\"abstract-sub-heading\\\">Aims</h3><p>Flower development influences reproduction and breeding in <i>E.agallocha</i>, which contributes to ecological restoration in the intertidal zone.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>We performed de novo transcriptome assembly analysis on male and female flowers and leaves from <i>E. agallocha</i>.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>We identified a total of 73 MADS-box genes in the <i>E. agallocha</i> genome, which we grouped into five distinct classes (MIKC<sup>c</sup>, Mα, Mβ, Mγ, MIKC*) after phylogenetic comparisons with <i>J. curcas</i> homologs. We analyzed expression profiles and suggested <i>AP1/FUL</i>, <i>AP3/PI</i>, <i>AGL104</i>, and <i>SOC1</i> as candidate regulators of sex determination. In addition, several <i>EaMADS</i> genes were significantly upregulated in flowers compared to leaves.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusion</h3><p>Our study represents the first detailed, comprehensive evaluation of the regulation of MADS-box genes associated with sex determination in <i>E. agallocha</i>. The assembled transcriptomic profiles increase the genetic information available for this species and constitute an important contribution to functional studies of inflorescence genes. In turn, this will help exploring the molecular mechanisms underlying the evolution of <i>E. agallocha</i>.</p>\",\"PeriodicalId\":7994,\"journal\":{\"name\":\"Annals of Forest Science\",\"volume\":\"11 6\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2022-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Forest Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1186/s13595-022-01156-6\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Forest Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s13595-022-01156-6","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
De novo transcriptome assembly reveals characteristics of flower sex determination of Excoecaria agallocha
Key message
MADS-box genes family may play important roles in the flower sex determination in Excoecaria agallocha. A total of 73 MADS-box genes were identified in E. agallocha. De novo transcriptome assembly analysis suggested that AP1/FUL, AP3/PI, AGL104, and SOC1 plays potential roles in E. agallocha flower sex determination.
Context
Excoecaria agallocha is a dioecious species containing both male and female individuals producing unisexual flowers. The underlying mechanisms regulating inflorescence differentiation in these species remains poorly understood.
Aims
Flower development influences reproduction and breeding in E.agallocha, which contributes to ecological restoration in the intertidal zone.
Methods
We performed de novo transcriptome assembly analysis on male and female flowers and leaves from E. agallocha.
Results
We identified a total of 73 MADS-box genes in the E. agallocha genome, which we grouped into five distinct classes (MIKCc, Mα, Mβ, Mγ, MIKC*) after phylogenetic comparisons with J. curcas homologs. We analyzed expression profiles and suggested AP1/FUL, AP3/PI, AGL104, and SOC1 as candidate regulators of sex determination. In addition, several EaMADS genes were significantly upregulated in flowers compared to leaves.
Conclusion
Our study represents the first detailed, comprehensive evaluation of the regulation of MADS-box genes associated with sex determination in E. agallocha. The assembled transcriptomic profiles increase the genetic information available for this species and constitute an important contribution to functional studies of inflorescence genes. In turn, this will help exploring the molecular mechanisms underlying the evolution of E. agallocha.
期刊介绍：
Annals of Forest Science is an official publication of the French National Institute for Agriculture, Food and Environment (INRAE)
-Up-to-date coverage of current developments and trends in forest research and forestry
Topics include ecology and ecophysiology, genetics and improvement, tree physiology, wood quality, and silviculture
-Formerly known as Annales des Sciences Forestières
-Biology of trees and associated organisms (symbionts, pathogens, pests)
-Forest dynamics and ecosystem processes under environmental or management drivers (ecology, genetics)
-Risks and disturbances affecting forest ecosystems (biology, ecology, economics)
-Forestry wood chain (tree breeding, forest management and productivity, ecosystem services, silviculture and plantation management)
-Wood sciences (relationships between wood structure and tree functions, and between forest management or environment and wood properties)
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