Y. Puchkova, N. Sedush, E. Kuznetsova, A. Nazarov, S. Chvalun
{"title":"用于改善奥沙利铂递送的PEG-b-PLA纳米颗粒的自组装行为和细胞毒性:PLA块长度的影响","authors":"Y. Puchkova, N. Sedush, E. Kuznetsova, A. Nazarov, S. Chvalun","doi":"10.1134/s2634827623600056","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The present study investigates the effect of the length of the hydrophobic poly(D,L-lactide) block on the self-assembly behavior of amphiphilic poly(D,L-lactide)-<i>b</i>-poly(ethylene glycol) (P(D,L)LA-<i>b</i>-PEG<sub>5k</sub>) copolymers with a large variation in molecular weight of the hydrophobic block (ranging from 5 to 100 kDa). To evaluate the cytotoxic effect of oxaliplatin-loaded P(D,L)LA-<i>b</i>-PEG<sub>5k</sub> nanoparticles (OXA-NPs) prepared by the nanoprecipitation method, a half maximal inhibitory concentration (IC<sub>50</sub>) study was conducted in cancer cell lines (MCF7, HCT116, and A549). The assays revealed that the IC<sub>50</sub> values for OXA-NPs in HCT116 (1.93 ± 0.08 nM) and A549 (2.84 ± 0.11 nM) were significantly lower than those of pure OXA (12.70 ± 1.14 and 29.30 ± 0.40 nM, respectively). Moreover, the results showed that normal fibroblasts treated with blank NPs exhibited no significant growth inhibition in the concentration range of 0.001–1 mg mL<sup>–1</sup>, indicating their high biocompatibility<i>.</i></p>","PeriodicalId":21086,"journal":{"name":"Reviews and Advances in Chemistry","volume":"4 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Self-Assembly Behavior and Cytotoxicity of PEG-b-PLA Nanoparticles for Improved Oxaliplatin Delivery: Effect of PLA Block Length\",\"authors\":\"Y. Puchkova, N. Sedush, E. Kuznetsova, A. Nazarov, S. Chvalun\",\"doi\":\"10.1134/s2634827623600056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>The present study investigates the effect of the length of the hydrophobic poly(D,L-lactide) block on the self-assembly behavior of amphiphilic poly(D,L-lactide)-<i>b</i>-poly(ethylene glycol) (P(D,L)LA-<i>b</i>-PEG<sub>5k</sub>) copolymers with a large variation in molecular weight of the hydrophobic block (ranging from 5 to 100 kDa). To evaluate the cytotoxic effect of oxaliplatin-loaded P(D,L)LA-<i>b</i>-PEG<sub>5k</sub> nanoparticles (OXA-NPs) prepared by the nanoprecipitation method, a half maximal inhibitory concentration (IC<sub>50</sub>) study was conducted in cancer cell lines (MCF7, HCT116, and A549). The assays revealed that the IC<sub>50</sub> values for OXA-NPs in HCT116 (1.93 ± 0.08 nM) and A549 (2.84 ± 0.11 nM) were significantly lower than those of pure OXA (12.70 ± 1.14 and 29.30 ± 0.40 nM, respectively). Moreover, the results showed that normal fibroblasts treated with blank NPs exhibited no significant growth inhibition in the concentration range of 0.001–1 mg mL<sup>–1</sup>, indicating their high biocompatibility<i>.</i></p>\",\"PeriodicalId\":21086,\"journal\":{\"name\":\"Reviews and Advances in Chemistry\",\"volume\":\"4 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reviews and Advances in Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1134/s2634827623600056\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reviews and Advances in Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s2634827623600056","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Self-Assembly Behavior and Cytotoxicity of PEG-b-PLA Nanoparticles for Improved Oxaliplatin Delivery: Effect of PLA Block Length
Abstract
The present study investigates the effect of the length of the hydrophobic poly(D,L-lactide) block on the self-assembly behavior of amphiphilic poly(D,L-lactide)-b-poly(ethylene glycol) (P(D,L)LA-b-PEG5k) copolymers with a large variation in molecular weight of the hydrophobic block (ranging from 5 to 100 kDa). To evaluate the cytotoxic effect of oxaliplatin-loaded P(D,L)LA-b-PEG5k nanoparticles (OXA-NPs) prepared by the nanoprecipitation method, a half maximal inhibitory concentration (IC50) study was conducted in cancer cell lines (MCF7, HCT116, and A549). The assays revealed that the IC50 values for OXA-NPs in HCT116 (1.93 ± 0.08 nM) and A549 (2.84 ± 0.11 nM) were significantly lower than those of pure OXA (12.70 ± 1.14 and 29.30 ± 0.40 nM, respectively). Moreover, the results showed that normal fibroblasts treated with blank NPs exhibited no significant growth inhibition in the concentration range of 0.001–1 mg mL–1, indicating their high biocompatibility.
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