Marlene K. Wolfe, Abigail Harvey Paulos, Alessandro Zulli, Dorothea Duong, Bridgette Shelden, Bradley J. White and Alexandria B. Boehm*,
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Wastewater Detection of Emerging Arbovirus Infections: Case Study of Dengue in the United States
Climate change and urbanization are increasing the distribution of insect vectors of infectious diseases. Dengue virus is an arbovirus that causes nearly 100 million symptomatic infections per year and is endemic in 124 countries, and the range of its mosquito vectors continues to increase. Surveillance of dengue virus infections is complicated by the fact that infections can be asymptomatic, and symptoms may not be readily recognizable to clinicians. Here we show that wastewater monitoring can be used to detect dengue virus RNA to yield information about the circulation of dengue infections in a community. We collected three samples of wastewater solids per week from three different wastewater treatment plants in Miami-Dade County, Florida, where dengue infections were locally acquired. Using molecular methods, we tested wastewater solids for RNA from the 4 dengue virus serotypes and consistently detected dengue virus 3 RNA at all three wastewater plants and did not detect the other 3 serotypes. According to publicly available data on dengue infections, a vast majority of infections were caused by serotype 3. Wastewater detection of dengue virus RNA is possible with as few as 4.23 laboratory confirmed dengue cases per 1 million people based on publicly available infection data.
期刊介绍:
Environmental Science & Technology Letters serves as an international forum for brief communications on experimental or theoretical results of exceptional timeliness in all aspects of environmental science, both pure and applied. Published as soon as accepted, these communications are summarized in monthly issues. Additionally, the journal features short reviews on emerging topics in environmental science and technology.