Odoemelam H. A., Wemedo S. A., Akani, N. P., Douglas S. I.
{"title":"从尼日利亚河流州新卡拉巴尔河捕捞的鲶鱼(Arius heudelotii)中分离和鉴定弧菌属","authors":"Odoemelam H. A., Wemedo S. A., Akani, N. P., Douglas S. I.","doi":"10.9734/sajrm/2023/v17i2325","DOIUrl":null,"url":null,"abstract":"This study was aimed at the isolation and identification of Vibrio spp. associated with Arius heudelotii, a catfish from a wild environment. Twenty-four Arius heudelotii (Catfish) were collected from the New Calabar River Choba, in Obio-Akpor LGA in Rivers State, Nigeria, and were processed to sample their skin, gills, and intestines. The Vibrio spp. associated with the gills, skins, and intestines of Arius heudelotii fish and water samples from the New Calabar River were isolated and identified. Ten Vibrio species (V. vulnificus, V. cholerae, V. parahaemolyticus, V. alginolyticus, V. mimicus, V. mytili, V. anguilarum, V. furnissii, V. diazotrophic, Alliivibrio fisheri, V. xuii and V. gazogenes) were isolated and presumptively identified based on the morphological and biochemical test. A total of 96 Vibrio species were isolated n=31 (32.4%), n=11 (11.5%), n=24 (25%), and n=30 (31.2%) for gills, skin, intestine, and water samples, respectively. Among the Vibrio species isolated V. parahaemolyticus 18.6%, was the most predominant, this was followed by V. Vulnificus 17.7%, V, Cholerae 14.6%, V. alginolyticus 11.6%, V. mimicus 8.3%, V. furnissii 7.3%, V. mytili 6.3%, V. anguilarum 6.3%, and Alliivibrio fisheri 6.3% and V. diazotrophic was the least predominant 3.1%. Vibrio organisms can be pathogenic. Therefore, it is important to investigate the presence of Vibrio spp. that are capable of causing sickness to humans and animals.","PeriodicalId":21776,"journal":{"name":"South Asian Journal of Research in Microbiology","volume":"69 8","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation and Identification of Vibrio Spp. from Catfish (Arius heudelotii) Harvested from New Calabar River in Rivers State, Nigeria\",\"authors\":\"Odoemelam H. A., Wemedo S. A., Akani, N. P., Douglas S. I.\",\"doi\":\"10.9734/sajrm/2023/v17i2325\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study was aimed at the isolation and identification of Vibrio spp. associated with Arius heudelotii, a catfish from a wild environment. Twenty-four Arius heudelotii (Catfish) were collected from the New Calabar River Choba, in Obio-Akpor LGA in Rivers State, Nigeria, and were processed to sample their skin, gills, and intestines. The Vibrio spp. associated with the gills, skins, and intestines of Arius heudelotii fish and water samples from the New Calabar River were isolated and identified. Ten Vibrio species (V. vulnificus, V. cholerae, V. parahaemolyticus, V. alginolyticus, V. mimicus, V. mytili, V. anguilarum, V. furnissii, V. diazotrophic, Alliivibrio fisheri, V. xuii and V. gazogenes) were isolated and presumptively identified based on the morphological and biochemical test. A total of 96 Vibrio species were isolated n=31 (32.4%), n=11 (11.5%), n=24 (25%), and n=30 (31.2%) for gills, skin, intestine, and water samples, respectively. Among the Vibrio species isolated V. parahaemolyticus 18.6%, was the most predominant, this was followed by V. Vulnificus 17.7%, V, Cholerae 14.6%, V. alginolyticus 11.6%, V. mimicus 8.3%, V. furnissii 7.3%, V. mytili 6.3%, V. anguilarum 6.3%, and Alliivibrio fisheri 6.3% and V. diazotrophic was the least predominant 3.1%. Vibrio organisms can be pathogenic. Therefore, it is important to investigate the presence of Vibrio spp. that are capable of causing sickness to humans and animals.\",\"PeriodicalId\":21776,\"journal\":{\"name\":\"South Asian Journal of Research in Microbiology\",\"volume\":\"69 8\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"South Asian Journal of Research in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/sajrm/2023/v17i2325\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"South Asian Journal of Research in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/sajrm/2023/v17i2325","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在从野外环境中分离和鉴定与黑斑鲶鱼相关的弧菌。从尼日利亚河流州Obio-Akpor LGA的New Calabar River Choba收集了24只Arius heudelotii(鲶鱼),并对其皮肤、鳃和肠道进行了处理。分离鉴定了新卡拉巴尔河(New Calabar River)黑鲈(Arius heudelotii)鱼鳃、皮、肠及水样中的弧菌属。通过形态学和生化检测,分离出10种弧菌(创伤弧菌、霍乱弧菌、副溶血性弧菌、溶藻弧菌、模拟弧菌、mytili弧菌、鳗弧菌、家蝇弧菌、重氮营养弧菌、渔业弧菌、xuii弧菌和gazogenes弧菌)。在鳃、皮肤、肠道和水样中分离到96种弧菌,分别为n=31(32.4%)、n=11(11.5%)、n=24(25%)和n=30(31.2%)。分离出的弧菌种类中,副溶血性弧菌最多,占总数的18.6%,其次是创伤弧菌17.7%、霍乱弧菌14.6%、溶藻弧菌11.6%、模拟弧菌8.3%、家粪弧菌7.3%、mytili弧菌6.3%、鳗弧菌6.3%、渔业弧菌6.3%,重氮营养弧菌占总数的3.1%。弧菌可能具有致病性。因此,重要的是调查是否存在能够引起人类和动物疾病的弧菌。
Isolation and Identification of Vibrio Spp. from Catfish (Arius heudelotii) Harvested from New Calabar River in Rivers State, Nigeria
This study was aimed at the isolation and identification of Vibrio spp. associated with Arius heudelotii, a catfish from a wild environment. Twenty-four Arius heudelotii (Catfish) were collected from the New Calabar River Choba, in Obio-Akpor LGA in Rivers State, Nigeria, and were processed to sample their skin, gills, and intestines. The Vibrio spp. associated with the gills, skins, and intestines of Arius heudelotii fish and water samples from the New Calabar River were isolated and identified. Ten Vibrio species (V. vulnificus, V. cholerae, V. parahaemolyticus, V. alginolyticus, V. mimicus, V. mytili, V. anguilarum, V. furnissii, V. diazotrophic, Alliivibrio fisheri, V. xuii and V. gazogenes) were isolated and presumptively identified based on the morphological and biochemical test. A total of 96 Vibrio species were isolated n=31 (32.4%), n=11 (11.5%), n=24 (25%), and n=30 (31.2%) for gills, skin, intestine, and water samples, respectively. Among the Vibrio species isolated V. parahaemolyticus 18.6%, was the most predominant, this was followed by V. Vulnificus 17.7%, V, Cholerae 14.6%, V. alginolyticus 11.6%, V. mimicus 8.3%, V. furnissii 7.3%, V. mytili 6.3%, V. anguilarum 6.3%, and Alliivibrio fisheri 6.3% and V. diazotrophic was the least predominant 3.1%. Vibrio organisms can be pathogenic. Therefore, it is important to investigate the presence of Vibrio spp. that are capable of causing sickness to humans and animals.