响应面法优化芽孢杆菌 TTMP20 的生长培养基

Shanling Gan, Lirong Luo, Xiaoxiao Ding, Yujia Li, Donglin Yang, Changhua Shang
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引用次数: 0

摘要

高密度培养能够获得较高的微生物浓度和活性,是发酵工业的关键技术。这将大大缩短发酵周期,降低生产成本。TTMP20是一株产2,3,5,6-四甲基吡嗪(TTMP)的高产菌株。本研究对芽孢杆菌sp. TTMP20的生长培养基进行了优化。研究了不同碳氮源,确定了最佳碳源(葡萄糖)和氮源(酵母浸膏)。以葡萄糖、酵母浸膏浓度和pH为影响因素,采用响应面法对上述影响因素进行优化,使菌株的细胞密度最大化。最佳生长培养基为酵母浸膏4 g/L、葡萄糖15.754 g/L、K2HPO4 2 g/L、NaCl 10 g/L、ph 6.50,预测最大值为1.388,实际最大值为1.3。这比基本培养基的细胞密度高61.4%。这将为TTMP的后续发酵提供充足的种子培养,提高芽孢杆菌sp. TTMP20的应用水平。
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Optimization of Growth Medium of Bacillus sp. TTMP20 by Response Surface Methodology
High-density culture is able to obtain high concentration and activity of microbes, which is a key technology for fermentation industry. This will greatly shorten fermentation cycle and reduce production cost. Bacillus sp. TTMP20 is a strain with a high yield of 2,3,5,6-tetramethylpyrazine (TTMP). In this study, the growth medium of Bacillus sp. TTMP20 was optimized. Different carbon/nitrogen sources were investigated, and the best carbon source (glucose) and nitrogen source (yeast extract) was determined. With the concentrations of glucose, yeast extract and pH as influence factors, response surface method was used to optimize the above-mentioned influence factors to maximize cell density of the strain. The optimal growth medium is as follows: 4 g/L of yeast extract, 15.754 g/L of glucose, 2 g/L of K2HPO4, 10 g/L of NaCl, and 6.50 of pH. The predicted and actual maxima are 1.388 and 1.3, respectively. This is 61.4% higher than the cell density of the basic medium. This will provide the adequate seed culture for the subsequent fermentation of TTMP, and improve the application level of Bacillus sp. TTMP20.
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
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发文量
60
审稿时长
6 months
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