利用血液学和抗 IgE 体液反应作为牛抗蜱表型标记的潜力

Collins Ngetich , Lucy Kamau , Jemimah Simbauni , Charles Mwendia , Milton Owido , Irene Kiio , Oswald Matika , Sarah Foster , Michael Birkett , Appolinaire Djikeng , Kellie Anne Watson , Naftaly Githaka
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引用次数: 0

摘要

全球大约80%的牛群面临着蜱虫和蜱传疾病(ttbd)的侵扰和感染风险。动物死亡、减产、病媒控制成本和动物治疗造成的经济损失非常巨大,因此迫切需要制定和部署替代病媒控制战略。培育宿主蜱虫抗性具有可持续大规模控制TTBD的潜力,特别是在牛中。测定牛身上蜱虫抗性表型的金标准方法是计数蜱虫,但这是非常费力和主观的。更客观、更快速、更大规模地对蜱虫抗性进行表型分析是选择宿主遗传抗性的关键。本研究研究了用脱色鼻头虫幼虫人工攻蜱后,印度白蜱和金牛群血液细胞特征、免疫反应(免疫球蛋白E、IgE)和全身蜱虫数量的相关性。54头弗里西亚和艾尔郡犊牛和52头东非Zebu犊牛各感染约2500只幼虫。从第20-25天开始,每天计数接近满的成年雌蜱(≥4.5 mm)。在试验第0天和第23天分别取血和血清进行细胞血和IgE滴度分析。与牛磺酸品种相比,食用牛对脱色红霉的抗性较强(P <0.0001)。侵染前血液成分的重复测量显示显著(P <0.05)与蜱虫感染后的IgE、红细胞、红细胞压积和血红蛋白计数有关。蜱虫数量与红细胞数量、血红蛋白、红细胞压积和IgE浓度之间也有很强的正相关(P <0.0001)。这种方法在表型宿主抗性方面的应用需要用更高的牛数和不同的蜱种或属进行评估。
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The potential for use of haematological and anti-IgE humoral responses as phenotypic markers for tick resistance in cattle

Approximately 80% of the global cattle population is at risk of infestation and infection by ticks and tick-borne diseases (TTBDs). The economic losses from animal mortality, reduced production, vector control costs and animal treatment are very substantial, hence there is an urgent need to develop and deploy alternative vector control strategies. Breeding for host tick resistance has the potential for sustainable large-scale TTBD control especially in cattle. The gold standard method for phenotyping tick resistance in cattle is by counting ticks on the body but is very laborious and subjective. Better methods for phenotyping tick resistance more objectively, faster and at scale, are essential for selecting host genetic resistance to ticks. This study investigated the correlation between haematological cellular profiles and immunological responses (immunoglobulin E, IgE) and full body tick counts in herds of Bos indicus and Bos taurus following artificial tick challenge with Rhipicephalus decoloratus larvae. Fifty-four Friesian and Ayrshire (Bos taurus) and 52 East African Zebu (Bos indicus) calves were each infested with ∼2500 larvae. Near-replete adult female ticks (≥ 4.5 mm) were counted daily from Day 20–25. Blood and serum samples were obtained from each animal on Days 0 and 23 for cellular blood and IgE titre analysis, respectively. The indicine cattle were refractory to R. decoloratus infestation in comparison with the taurine breed (P < 0.0001). Repeated measurements of blood components pre-infestation revealed a significant (P < 0.05) association with tick count in IgE and red blood cells, haematocrit, and haemoglobin post-infestation. There was also a strong positive correlation between the tick counts and red blood cell numbers, haemoglobin, haematocrit, and IgE concentration (P < 0.0001) following tick challenge. The application of this approach to phenotype host resistance needs to be assessed using higher cattle numbers and with different tick species or genera.

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