Wu Lijuan , Han Cong , Wang Huimei , He Yuchang , Lin Hai , Wang Lei , Chen Chen , E. Zhiguo
{"title":"OsbZIP53 通过参与活性氧和水杨酸代谢负向调节水稻的免疫反应","authors":"Wu Lijuan , Han Cong , Wang Huimei , He Yuchang , Lin Hai , Wang Lei , Chen Chen , E. Zhiguo","doi":"10.1016/j.rsci.2023.12.002","DOIUrl":null,"url":null,"abstract":"<div><p>The basic region/leucine zipper (bZIP) transcription factors play important roles in plant development and responses to abiotic and biotic stresses. OsbZIP53 regulates resistance to <em>Magnaporthe oryzae</em> in rice by analyzing <em>APIP5</em>-RNAi transgenic plants. To further investigate the biological functions of <em>OsbZIP53</em>, we generated <em>osbzip53</em> mutants using CRISPR/Cas9 editing and also constructed <em>OsbZIP53</em> over-expression transgenic plants. Comprehensive analysis of phenotypical, physiological, and transcriptional data showed that knocking-out <em>OsbZIP53</em> not only improved disease resistance by inducing a hypersensitivity response in plants, but also regulated the immune response through the salicylic acid pathway. Specifically, disrupting <em>OsbZIP53</em> increased H<sub>2</sub>O<sub>2</sub> accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs (<em>Osrboh</em> genes) and weakened H<sub>2</sub>O<sub>2</sub> degradation by directly targeting <em>OsMYBS1</em>. In addition, the growth of <em>osbzip53</em> mutants was seriously impaired, while <em>OsbZIP53</em> over-expression lines displayed a similar phenotype to the wild type, suggesting that <em>OsbZIP53</em> has a balancing effect on rice immune response and growth.</p></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 2","pages":"Pages 190-202"},"PeriodicalIF":5.6000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1672630823001245/pdfft?md5=26d406cfe1c9fd16288d55f3dd2f4783&pid=1-s2.0-S1672630823001245-main.pdf","citationCount":"0","resultStr":"{\"title\":\"OsbZIP53 Negatively Regulates Immunity Response by Involving in Reactive Oxygen Species and Salicylic Acid Metabolism in Rice\",\"authors\":\"Wu Lijuan , Han Cong , Wang Huimei , He Yuchang , Lin Hai , Wang Lei , Chen Chen , E. Zhiguo\",\"doi\":\"10.1016/j.rsci.2023.12.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The basic region/leucine zipper (bZIP) transcription factors play important roles in plant development and responses to abiotic and biotic stresses. OsbZIP53 regulates resistance to <em>Magnaporthe oryzae</em> in rice by analyzing <em>APIP5</em>-RNAi transgenic plants. To further investigate the biological functions of <em>OsbZIP53</em>, we generated <em>osbzip53</em> mutants using CRISPR/Cas9 editing and also constructed <em>OsbZIP53</em> over-expression transgenic plants. Comprehensive analysis of phenotypical, physiological, and transcriptional data showed that knocking-out <em>OsbZIP53</em> not only improved disease resistance by inducing a hypersensitivity response in plants, but also regulated the immune response through the salicylic acid pathway. Specifically, disrupting <em>OsbZIP53</em> increased H<sub>2</sub>O<sub>2</sub> accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs (<em>Osrboh</em> genes) and weakened H<sub>2</sub>O<sub>2</sub> degradation by directly targeting <em>OsMYBS1</em>. In addition, the growth of <em>osbzip53</em> mutants was seriously impaired, while <em>OsbZIP53</em> over-expression lines displayed a similar phenotype to the wild type, suggesting that <em>OsbZIP53</em> has a balancing effect on rice immune response and growth.</p></div>\",\"PeriodicalId\":56069,\"journal\":{\"name\":\"Rice Science\",\"volume\":\"31 2\",\"pages\":\"Pages 190-202\"},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1672630823001245/pdfft?md5=26d406cfe1c9fd16288d55f3dd2f4783&pid=1-s2.0-S1672630823001245-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rice Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1672630823001245\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rice Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1672630823001245","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
OsbZIP53 Negatively Regulates Immunity Response by Involving in Reactive Oxygen Species and Salicylic Acid Metabolism in Rice
The basic region/leucine zipper (bZIP) transcription factors play important roles in plant development and responses to abiotic and biotic stresses. OsbZIP53 regulates resistance to Magnaporthe oryzae in rice by analyzing APIP5-RNAi transgenic plants. To further investigate the biological functions of OsbZIP53, we generated osbzip53 mutants using CRISPR/Cas9 editing and also constructed OsbZIP53 over-expression transgenic plants. Comprehensive analysis of phenotypical, physiological, and transcriptional data showed that knocking-out OsbZIP53 not only improved disease resistance by inducing a hypersensitivity response in plants, but also regulated the immune response through the salicylic acid pathway. Specifically, disrupting OsbZIP53 increased H2O2 accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs (Osrboh genes) and weakened H2O2 degradation by directly targeting OsMYBS1. In addition, the growth of osbzip53 mutants was seriously impaired, while OsbZIP53 over-expression lines displayed a similar phenotype to the wild type, suggesting that OsbZIP53 has a balancing effect on rice immune response and growth.
Rice ScienceAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
8.90
自引率
6.20%
发文量
55
审稿时长
40 weeks
期刊介绍：
Rice Science is an international research journal sponsored by China National Rice Research Institute. It publishes original research papers, review articles, as well as short communications on all aspects of rice sciences in English language. Some of the topics that may be included in each issue are: breeding and genetics, biotechnology, germplasm resources, crop management, pest management, physiology, soil and fertilizer management, ecology, cereal chemistry and post-harvest processing.
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