光动力作用对革兰氏阴性菌纯培养物和混合培养物的影响:与生长机制有关

IF 1.4 4区 物理与天体物理 Q3 OPTICS Laser Physics Letters Pub Date : 2023-12-14 DOI:10.1088/1612-202x/ad12b0
Rebeca V de Lima, Jennifer M Soares, Kate C Blanco, Vanderlei S Bagnato
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引用次数: 0

摘要

革兰氏阴性细菌的细胞壁结构复杂,有别于革兰氏阳性细菌,因此对传统治疗方法构成了巨大挑战。这些结构上的差异加上各种抗菌机制,使它们具有很强的抵抗力。光动力灭活(PDI)利用光和光敏剂(PS)协同作用诱导的氧化应激,已成为应对这一挑战的一项前景广阔的技术。在本研究中,我们深入研究了 PDI 的应用及其对肺炎克雷伯菌和大肠埃希菌纯培养物和混合菌群的影响。我们的研究包括分析受光动力影响时生长曲线的变化以及对庆大霉素敏感性的反应。研究方法不同浓度(50 μM、100 μM 和 150 μM)的合成姜黄素溶液在治疗组中用作 PS。此外,还对二甲基亚砜和 0.1% 十二烷基硫酸钠(SDS)等溶剂进行了评估,以增强 PS 的流动性和吸收性。建立了纯培养物和混合培养物在 PDI 前后以及 0.1% SDS 存在下的生长曲线。采用 30 J cm-2 和 450 nm 的蓝光照射。在有 PDI + SDS 和没有 PDI + SDS 的情况下,测定了抗生素的最低抑菌浓度 (MIC)。结果:虽然 PDI 并未对这些菌株达到最佳的细菌减少效果,但它确实带来了氧化损伤,有可能影响培养物的时间进展及其对抗生素反应的其他关键方面。结论这项研究表明,即使在 0.1% SDS 存在的情况下 PDI 的影响很小,细菌生长曲线和 MIC 值也会发生明显的变化,可用于治疗此类感染。
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Influence of photodynamic action on pure and mixed cultures of gram-negative bacteria: related to growth mechanisms
Gram-negative bacteria present a significant challenge to conventional treatments due to their structurally complex cell walls, setting them apart from their gram-positive counterparts. These structural distinctions, coupled with various antimicrobial resistance mechanisms, make them highly resilient. Photodynamic inactivation (PDI) has emerged as a promising technique to address this challenge, capitalizing on oxidative stress induced by the synergy of light and a photosensitizer (PS). In this study, we delved into the application of PDI and its repercussions on both Klebsiella pneumoniae and Escherichia coli bacteria, in both pure cultures and mixed populations. Our investigation encompassed an analysis of changes in growth curves when influenced by photodynamic and the response to the susceptibility to gentamicin. Methods: Various concentrations (50 μM, 100 μM, and 150 μM) of synthetic curcumin solutions served as PS in treatment groups. Additionally, solvents such as dimethyl sulfoxide and 0.1% sodium dodecyl sulfate (SDS) were evaluated to enhance PS mobility and absorption. Growth curves for pure and mixed cultures were established, both pre- and post-PDI, and in the presence of 0.1% SDS. Blue light irradiation at 30 J cm−2 and 450 nm was employed. The minimum inhibitory concentration (MIC) of the antibiotic was determined with and without PDI + SDS. Results: While PDI did not achieve optimal bacterial reduction for these strains, it did introduce oxidative damage that has the potential to affect other critical aspects of the temporal progression of cultures and their responses to antibiotics. Conclusion: This study shows that even with the minimal impact of PDI in the presence of 0.1% SDS, observable alterations in bacterial growth profiles and MIC values occur and can be used in favor of treatment involving such infections.
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来源期刊
Laser Physics Letters
Laser Physics Letters 物理-仪器仪表
CiteScore
3.30
自引率
11.80%
发文量
174
审稿时长
2.4 months
期刊介绍: Laser Physics Letters encompasses all aspects of laser physics sciences including, inter alia, spectroscopy, quantum electronics, quantum optics, quantum electrodynamics, nonlinear optics, atom optics, quantum computation, quantum information processing and storage, fiber optics and their applications in chemistry, biology, engineering and medicine. The full list of subject areas covered is as follows: -physics of lasers- fibre optics and fibre lasers- quantum optics and quantum information science- ultrafast optics and strong-field physics- nonlinear optics- physics of cold trapped atoms- laser methods in chemistry, biology, medicine and ecology- laser spectroscopy- novel laser materials and lasers- optics of nanomaterials- interaction of laser radiation with matter- laser interaction with solids- photonics
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