利用 QuEChERS 和超高效液相色谱-四极杆/比特高分辨质谱法同时检测葡萄中的 22 种霉菌毒素

IF 5.2 Q1 FOOD SCIENCE & TECHNOLOGY Journal of Future Foods Pub Date : 2023-12-14 DOI:10.1016/j.jfutfo.2023.11.009
Meirong Cao , Jing Wang , Mengtian Wang , Xiaoxuan Yuan , Xu Zhang , Junzhan Ma , Yan Zhang , Qiang Li
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引用次数: 0

摘要

为了有效评估葡萄中霉菌毒素的污染程度,我们开发了一种超高效液相色谱-四极杆/比特阱高分辨质谱(UPLC-Q-Orbitrap HRMS)分析 22 种霉菌毒素的方法。样品采用 QuEChERS(快速、简便、廉价、有效、耐用和安全)程序进行提取和纯化。提取物在 BEH Shield C18 色谱柱(100 mm × 2.1 mm, 1.7 μm)上分离,流动相为甲醇和含 0.1% 甲酸溶液和 2 mmol/L 乙酸铵的水。采用基质匹配外标法进行定量。22 种霉菌毒素在各自的质量浓度范围内线性关系良好,相关系数不小于 0.998 1。定量限为 0.3-10.0 μg/kg。霉菌毒素的回收率为 68.6%~109.0%,相对标准偏差为 1.28%~12.80%。该方法简单、快速、灵敏度高且准确,可用于筛选葡萄中常见的和新出现的霉菌毒素。
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Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry

To effectively assess the extent of the mycotoxin contamination of grapes, a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). Samples were extracted and purified using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure. The extracts were separated on a BEH Shield C18 column (100 mm × 2.1 mm, 1.7 μm), using methanol and water with 0.1% formic acid solution and 2 mmol/L ammonium acetate as the mobile phases. The quantification was performed according to a matrix-matched external standard method. There was a good linear relationship within the respective mass concentration ranges of 22 mycotoxins; the correlation coefficients were not less than 0.998 1. The limit of quantification was 0.3–10.0 μg/kg. Mycotoxin recovery ranged from 68.6% to 109.0%, with a relative standard deviation of 1.28%–12.80%. The method is simple, rapid, highly sensitive, and accurate, making it useful for screening grapes for common and emerging mycotoxins.

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