雌激素是听觉健康的守护者:Tsp1-CD47轴调节与噪音诱发的听力损失

IF 2.9 4区 医学 Q1 OBSTETRICS & GYNECOLOGY Climacteric Pub Date : 2023-12-18 DOI:10.1080/13697137.2023.2287632
X Wang, H Zheng, B Yang, M Zu, Z Wang, J Zhang, F Zheng, M Yang, M C F Tong, L Zhao, W Bai
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引用次数: 0

摘要

研究目的本研究旨在分析雌激素在噪声性听力损失(NIHL)中的作用,并揭示其潜在机制:方法:构建卵巢切除的 Sprague-Dawley 大鼠(OVX)模型,使用听性脑干反应(ABR)测试法研究噪声暴露前后的听阈和听觉潜伏期。使用免疫荧光、扫描电子显微镜和透射电子显微镜评估了大鼠的形态学变化。蛋白质组学和生物信息学用于分析其机制。研究结果通过 Western 印迹和 Luminex 液体悬浮芯片技术得到进一步验证:结果:暴露于噪声后,OVX 大鼠的听阈大幅升高。结果:暴露于噪声后,OVX 大鼠的听阈显著升高,ABR 波 I 潜伏期明显延迟,同时外毛细胞丢失增加,立体纤毛严重塌陷,表皮板明显变形。因此,补充雌激素的卵巢切除大鼠表现出对 NIHL 的耐受性。此外,研究还发现并验证了OVX大鼠体内血栓软骨素1(Tsp1)-CD47轴的显著上调:结论:OVX 大鼠更易患 NIHL,而雌激素的保护作用是通过调节 Tsp1-CD47 轴实现的。这项研究提出了雌激素调节 NIHL 的新机制,为临床治疗 NIHL 提供了一种潜在的干预策略。
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Estrogen as a guardian of auditory health: Tsp1-CD47 axis regulation and noise-induced hearing loss.

Objectives: This study aimed to analyze the role of estrogen in noise-induced hearing loss (NIHL) and uncover underlying mechanisms.

Methods: An ovariectomized Sprague-Dawley rat model (OVX) was constructed to investigate the hearing threshold and auditory latency before and after noise exposure using the auditory brainstem response (ABR) test. The morphological changes were assessed using immunofluorescence, scanning electron microscopy and transmission electron microscopy. Proteomics and bioinformatics were used to analyze the mechanism. The findings were further verified through western blot and Luminex liquid suspension chip technology.

Results: After noise exposure, OVX rats exhibited substantially elevated hearing thresholds. A conspicuous delay in ABR wave I latency was observed, alongside increased loss of outer hair cells, severe collapse of stereocilia and pronounced deformation of the epidermal plate. Accordingly, OVX rats with estrogen supplementation exhibited tolerance to NIHL. Additionally, a remarkable upregulation of the thrombospondin 1 (Tsp1)-CD47 axis in OVX rats was discovered and verified.

Conclusions: OVX rats were more susceptible to NIHL, and the protective effect of estrogen was achieved through regulation of the Tsp1-CD47 axis. This study presents a novel mechanism through which estrogen regulates NIHL and offers a potential intervention strategy for the clinical treatment of NIHL.

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来源期刊
Climacteric
Climacteric 医学-妇产科学
CiteScore
1.70
自引率
7.10%
发文量
53
审稿时长
1 months
期刊介绍: Climacteric is the official journal of the International Menopause Society (IMS). As an international peer-reviewed journal it publishes original research and reviews of all aspects of aging in women. Climacteric was founded by the IMS in 1998 and today has become a leading journal in the publication of peer-reviewed papers on the menopause, climacteric and mid-life health. Topics covered include endocrine changes, symptoms attributed to the menopause and their treatment, hormone replacement and alternative therapies, lifestyles, and the counselling and education of peri- and postmenopausal women. Climacteric, published bimonthly, also features regular invited reviews, editorials and commentaries on recent developments. The editorial review board of Climacteric includes leading scientific and clinical experts in the field of midlife medicine and research and is headed by its Editor-in-Chief, Professor Rod Baber of Australia. He and his team of Associate Editors act independently to set a clear editorial policy, co-ordinate peer review, and ensure a rapid response to submitted papers.
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