Glory Jesutomisin Ojo, Olugbenga Samson Onile, Abdul Onoruoiza Momoh, Bolaji Fatai Oyeyemi, Victor Omoboyede, Adeyinka Ignatius Fadahunsi, Tolulope Onile
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To this end, soil and water samples were collected from Ilesha gold mining, Osun State, Nigeria, and they were subjected to various analyses aimed at evaluating their various physicochemical parameters, heavy metal concentration, heavy metal-resistant bacteria isolation, and other analyses which culminated in the molecular characterization of heavy metal-resistant bacteria.</p><p><strong>Results: </strong>Notably, the results obtained from this study revealed that the concentration of heavy metal in the water samples around the mining site was in the order Co > Zn > Cd > Pb > Hg while that of the soil samples was in the order Co > Cd > Pb > Hg > Zn. A minimum inhibitory concentration test performed on the bacteria isolates from the samples revealed some of the isolates could resist as high as 800 ppm of Co, Cd, and Zn, 400 ppm, and 100 ppm of Pb and Hg respectively. Molecular characterization of the isolates revealed them as Priestia aryabhattai and Enterobacter cloacae.</p><p><strong>Conclusion: </strong>Further analysis revealed the presence of heavy metal-resistant genes (HMRGs) including merA, cnrA, and pocC in the isolated Enterobacter cloacae. Ultimately, the bacteria identified in this study are good candidates for bioremediation and merit further investigation in efforts to bioremediate heavy metals in gold mining sites.</p>","PeriodicalId":74026,"journal":{"name":"Journal, genetic engineering & biotechnology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746654/pdf/","citationCount":"0","resultStr":"{\"title\":\"Physiochemical analyses and molecular characterization of heavy metal-resistant bacteria from Ilesha gold mining sites in Nigeria.\",\"authors\":\"Glory Jesutomisin Ojo, Olugbenga Samson Onile, Abdul Onoruoiza Momoh, Bolaji Fatai Oyeyemi, Victor Omoboyede, Adeyinka Ignatius Fadahunsi, Tolulope Onile\",\"doi\":\"10.1186/s43141-023-00607-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The contribution of the processes involved and waste generated during gold mining to the increment of heavy metals concentration in the environment has been well established. 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引用次数: 0
摘要
背景:金矿开采过程中所涉及的工序和产生的废物对环境中重金属浓度的增加所起的作用已得到公认。虽然某些重金属是生物体正常运作所必需的,但某些重金属已被确认会对生态系统产生有害影响,并在生物体内发挥非生理作用。因此,努力降低重金属浓度水平至关重要。为此,我们从尼日利亚奥逊州伊莱沙金矿采集了土壤和水样本,并对它们进行了各种分析,旨在评估它们的各种理化参数、重金属浓度、抗重金属细菌分离以及其他分析,最终确定了抗重金属细菌的分子特征:值得注意的是,研究结果显示,矿区周围水样中的重金属浓度依次为 Co > Zn > Cd > Pb > Hg,而土壤样本中的重金属浓度依次为 Co > Cd > Pb > Hg > Zn。对样本中分离出的细菌进行的最低抑菌浓度测试表明,其中一些分离菌株对钴、镉和锌的抑菌浓度分别高达百万分之 800、百万分之 400 和百万分之 100,对铅和汞的抑菌浓度也分别高达百万分之 400 和百万分之 100。分离物的分子特征显示它们是 Priestia aryabhattai 和 Enterobacter cloacae:结论:进一步分析表明,分离出的泄殖腔肠杆菌中存在抗重金属基因(HMRGs),包括 merA、cnrA 和 pocC。最终,本研究中发现的细菌是生物修复的良好候选菌,值得在金矿开采地重金属生物修复工作中进一步研究。
Physiochemical analyses and molecular characterization of heavy metal-resistant bacteria from Ilesha gold mining sites in Nigeria.
Background: The contribution of the processes involved and waste generated during gold mining to the increment of heavy metals concentration in the environment has been well established. While certain heavy metals are required for the normal functioning of an organism, certain heavy metals have been identified for their deleterious effects on the ecosystem and non-physiological roles in organisms. Hence, efforts aimed at reducing their concentration level are crucial. To this end, soil and water samples were collected from Ilesha gold mining, Osun State, Nigeria, and they were subjected to various analyses aimed at evaluating their various physicochemical parameters, heavy metal concentration, heavy metal-resistant bacteria isolation, and other analyses which culminated in the molecular characterization of heavy metal-resistant bacteria.
Results: Notably, the results obtained from this study revealed that the concentration of heavy metal in the water samples around the mining site was in the order Co > Zn > Cd > Pb > Hg while that of the soil samples was in the order Co > Cd > Pb > Hg > Zn. A minimum inhibitory concentration test performed on the bacteria isolates from the samples revealed some of the isolates could resist as high as 800 ppm of Co, Cd, and Zn, 400 ppm, and 100 ppm of Pb and Hg respectively. Molecular characterization of the isolates revealed them as Priestia aryabhattai and Enterobacter cloacae.
Conclusion: Further analysis revealed the presence of heavy metal-resistant genes (HMRGs) including merA, cnrA, and pocC in the isolated Enterobacter cloacae. Ultimately, the bacteria identified in this study are good candidates for bioremediation and merit further investigation in efforts to bioremediate heavy metals in gold mining sites.