JunB 缩合可减轻高血糖条件下的血管内皮损伤。

IF 5.3 2区 生物学 Q2 CELL BIOLOGY Journal of Molecular Cell Biology Pub Date : 2024-04-10 DOI:10.1093/jmcb/mjad072
Xuxia Ren, Zexu Cui, Qiaoqiao Zhang, Zhiguang Su, Wei Xu, Jinhui Wu, Hao Jiang
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引用次数: 0

摘要

内皮损伤是糖尿病患者血管并发症发生和发展的最初和关键因素,也是导致发病率和死亡率的原因之一。虽然高血糖已被确定为一种损伤效应因子,但其详细机制仍难以捉摸。本研究通过 ATAC-seq 和 RNA-seq 发现,JunB 主要通过细胞周期和 p53 信号通路逆转了高糖处理的人脐静脉内皮细胞的增殖抑制和凋亡促进作用。此外,JunB 在其内在无序区和 DNA 结合域的介导下,会在细胞核内和体外发生相分离。JunB介导的增殖和凋亡需要核定位和凝集行为。因此,我们的研究揭示了 JunB 及其共轭物在修复高糖引起的血管内皮损伤中的作用,阐明了相分离在糖尿病和糖尿病内皮功能障碍中的参与。
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JunB condensation attenuates vascular endothelial damage under hyperglycemic condition.

Endothelial damage is the initial and crucial factor in the occurrence and development of vascular complications in diabetic patients, contributing to morbidity and mortality. Although hyperglycemia has been identified as a damaging effector, the detailed mechanisms remain elusive. In this study, identified by ATAC-seq and RNA-seq, JunB reverses the inhibition of proliferation and the promotion of apoptosis in human umbilical vein endothelial cells treated with high glucose, mainly through the cell cycle and p53 signaling pathways. Furthermore, JunB undergoes phase separation in the nucleus and in vitro, mediated by its intrinsic disordered region and DNA-binding domain. Nuclear localization and condensation behaviors are required for JunB-mediated proliferation and apoptosis. Thus, our study uncovers the roles of JunB and its coacervation in repairing vascular endothelial damage caused by high glucose, elucidating the involvement of phase separation in diabetes and diabetic endothelial dysfunction.

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来源期刊
CiteScore
9.60
自引率
1.80%
发文量
1383
期刊介绍: The Journal of Molecular Cell Biology ( JMCB ) is a full open access, peer-reviewed online journal interested in inter-disciplinary studies at the cross-sections between molecular and cell biology as well as other disciplines of life sciences. The broad scope of JMCB reflects the merging of these life science disciplines such as stem cell research, signaling, genetics, epigenetics, genomics, development, immunology, cancer biology, molecular pathogenesis, neuroscience, and systems biology. The journal will publish primary research papers with findings of unusual significance and broad scientific interest. Review articles, letters and commentary on timely issues are also welcome. JMCB features an outstanding Editorial Board, which will serve as scientific advisors to the journal and provide strategic guidance for the development of the journal. By selecting only the best papers for publication, JMCB will provide a first rate publishing forum for scientists all over the world.
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