从黄花蒿中分离出的倍半萜对 3T3-L1 脂肪细胞成脂分化的抑制作用

IF 4.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY International Journal of Molecular Sciences Pub Date : 2023-12-22 DOI:10.3390/ijms25010200
J. Lee, Jung Hwan Oh, F. Karadeniz, Chang-Suk Kong, Y. Seo
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引用次数: 0

摘要

肥胖症及相关并发症是现代社会的重大健康问题,主要归因于久坐不动的生活方式和富含碳水化合物的饮食。由于抗肥胖药物通常会产生严重的副作用,因此全球都在寻求预防措施,包括改变饮食习惯和食用能够抑制体重增加的功能性食品。在这种情况下,人们广泛研究了植物代谢物对肥胖症的有利生物效应。据报道,蒿属的几种植物具有抗脂肪生成的特性,可防止脂肪细胞成熟和积累脂质。本研究调查了从蒿属植物中分离出来的两种倍半萜类化合物--雷诺素和山奈素--在脂肪诱导的 3T3-L1 前脂肪细胞中的抗致脂潜力。与未经处理的成熟脂肪细胞相比,经这些分离化合物处理的分化 3T3-L1 脂肪细胞显示出较少的成脂特征。结果表明,与未经处理的对照组脂肪细胞相比,经雷诺素和金刚烷处理的细胞积累的细胞内脂质分别减少了 55.0% 和 52.5%。此外,在分化的脂肪细胞中,经 60 μM 雷诺素和金刚烷胺处理后,关键的成脂标志物--转录因子 PPARγ 的 mRNA 表达分别被抑制了 87.2% 和 91.7%。蛋白质表达也受到类似的抑制,60 μM 雷诺诺素和金刚烷胺处理后分别抑制了 92.7% 和 82.5%。同样,用样品处理的脂肪细胞在分化过程中,SERBP1c 和 C/EBPα 的基因和蛋白水平也被下调。进一步分析表明,这些化合物的抗脂肪生成作用可能是 AMPK 激活和随后抑制 MAPK 磷酸化的结果。总之,本研究表明倍半萜类化合物、雷诺素和山奈素是两种具有抗脂肪生成特性的潜在生物活性化合物。还需要进一步的研究来探索 A. scoparia 中的其他生物活性物质,并阐明 reynosin 和 santamarine 的体内作用机制。
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Inhibitory Effects of Sesquiterpenoids Isolated from Artemisia scoparia on Adipogenic Differentiation of 3T3-L1 Preadipocytes
Obesity and related complications are significant health issues in modern society, largely attributed to a sedentary lifestyle and a carbohydrate-rich diet. Since anti-obesity drugs often come with severe side effects, preventative measures are being sought globally, including dietary changes and functional foods that can counteract weight gain. In this context, plant-based metabolites are extensively studied for their advantageous biological effects against obesity. Several plants within the Artemisia genus have been reported to possess anti-adipogenic properties, preventing adipocytes from maturing and accumulating lipids. The present study investigated the anti-adipogenic potential of two sesquiterpenoids, reynosin and santamarine, isolated from A. scoparia in adipose-induced 3T3-L1 preadipocytes. Differentiating 3T3-L1 adipocytes treated with these isolated compounds displayed fewer adipogenic characteristics compared to untreated mature adipocytes. The results indicated that cells treated with reynosin and santamarine accumulated 55.0% and 52.5% fewer intracellular lipids compared to untreated control adipocytes, respectively. Additionally, the mRNA expression of the key adipogenic marker, transcription factor PPARγ, was suppressed by 87.2% and 91.7% following 60 μM reynosin and santamarine treatment, respectively, in differentiated adipocytes. Protein expression was also suppressed in a similar manner, at 92.7% and 82.5% by 60 μM reynosin and santamarine treatment, respectively. Likewise, SERBP1c and C/EBPα were also downregulated at both gene and protein levels in adipocytes treated with samples during differentiation. Further analysis suggested that the anti-adipogenic effect of the compounds might be a result of AMPK activation and the subsequent suppression of MAPK phosphorylation. Overall, the present study suggested that sesquiterpenoids, reynosin, and santamarine were two potential bioactive compounds with anti-adipogenic properties. Further research is needed to explore other bioactive agents within A. scoparia and elucidate the in vivo action mechanisms of reynosin and santamarine.
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来源期刊
International Journal of Molecular Sciences
International Journal of Molecular Sciences Chemistry-Organic Chemistry
CiteScore
8.10
自引率
10.70%
发文量
13472
审稿时长
17.49 days
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
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