干扰素 γ 和 NKG2D 受体基因多态性在预测艾滋病毒/HCV 合并感染垂直传播中的作用

A. A. Khamatova, I. Balmasova, T. A. Chebotareva
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摘要

目的:研究IFNγ基因变异体rs2430561和NKG2D基因变异体rs2617160的单核苷酸多态性作为HIV/HCV共感染妇女母婴病毒传播的潜在危险因素。材料和方法:研究对象为65对母婴,根据儿童感染情况分为4组(HIV/HCV感染、HIV或HCV感染、未感染)。方法:多重聚合酶链反应(PCR)、用于确定 HIV 和 HCV 病毒载量的 PCR、用于免疫学研究的流式细胞术、统计分析。结果:IFNγ 基因变异体 rs2430561 的单核苷酸多态性在确定母亲时没有预后价值。在记录孩子体内该基因单核苷酸多态性的 TT 和 AT 基因型时,根据怀孕不同孕期母亲血液中 HIV 和 HCV 病毒载量的大小,结合孩子感染 HIV/HCV、HIV 或 HCV 的概率。母亲体内存在的 NKG2D 基因变异体 rs2617160 的 AA 和 AT 基因型对儿童感染 HIV/HCV 或仅感染 HIV 有预后价值。结论:合并感染艾滋病毒/HCV 的妇女体内存在 NKG2D 基因变异体 rs2617160 的 AA 和 AT 基因型,不仅可以在孕期预测儿童感染的风险,还可以在计划阶段预测儿童感染的风险。
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Polymorphisms of interferon γ and NKG2D receptor genes in predicting vertical transmission of HIV/HCV coinfection
Objective: to investigate single nucleotide polymorphisms in IFNγ gene variant rs2430561 and in NKG2D gene variant rs2617160 as potential risk factors for mother-to-child viral transmission among HIV/HCV-coinfected women.Materials and methods: 65 mother-child pairs were examined, divided into 4 groups depending on the child's infection (HIV/ HCV infected, HIV or HCV infected, not infected). Methods: multiplex polymerase chain reaction (PCR), PCR for determining the viral load of HIV and HCV, flow cytometry for immunological studies, statistical analysis.Results: the single nucleotide polymorphism in IFNγ gene variant rs2430561 had no prognostic value when determined in the mothers. When recording the TT and AT genotypes of a single nucleotide polymorphism in this gene in a child, it was combined with the probability of the child's infection with HIV/HCV, HIV or HCV, depending on the magnitude of the viral load of HIV and HCV in the mother's blood in different trimesters of pregnancy. The presence of the AA and AT genotypes of the NKG2D gene variant rs2617160 in the mothers had a prognostic value as contributing tothe child infection with HIV/HCV or HIV alone. It was noted that the mechanisms of perinatal viral transmission included a certain level of decline in the absolute number of CD4+ lymphocytes in the woman blood in the second and third trimesters of pregnancy.Conclusions: the presence of AA and AT genotypes of the NKG2D gene variant rs2617160 in woman co-infected with HIV/HCV makes it possible to predict the risk of child infection not only during pregnancy, but also at the planning stage.
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