培养基和人工光照类型对微繁殖欧蓬植物色素沉着的影响

IF 3.1 3区 农林科学 Q1 HORTICULTURE Horticulturae Pub Date : 2023-12-17 DOI:10.3390/horticulturae9121348
Hector Silos Espino, R. Escalera García, D. Moncada González, L. L. Valera-Montero, S. Flores Benítez, M. Ortiz Morales, H. S. Guzmán Maldonado, Nivia Escalante-García, E. Olvera-Gonzalez
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引用次数: 0

摘要

欧当归因其营养、功能和治疗特性,以及在制药、化妆品和生物能源行业的潜力,在经济上被公认为一种重要的作物。欧蓬属植物的传统繁殖方法是种子繁殖或通过生根接穗或嫁接进行无性繁殖。然而,繁殖程序不足以进行广泛传播。体外繁殖是替代技术之一。因此,我们的目标是评估不同成分的生长与光照条件的结合情况,以诱导茧、胚胎发生以及欧蓬蒂属植物中一种成分的可能合成。此外,我们还分析了不同颜色果实基因型中是否存在参与色素合成的基因。在这项研究中,我们使用了果糖(2 克/升)与刺梨汁(2 毫升/升)、甘露醇(4 克/升)、硝酸银(AgNO3:1 毫克/升)、2,4-二氯苯氧乙酸(2,4-D:2 毫克/升)和吲哚-3-乙酸(IAA:1 克/升)的不同组合,以及白色、蓝色和红色 LED(发光二极管)和激光束。这些外植体产生了足够的单酚、没食子酸当量(GAE:2283.30 ± 6.29 mg/100 g)、香豆酸(2155.0 ± 35.0 mg/100 g)和阿魏酸(2176 ± 27.9 mg/100 g)(基因型为 Tapón aguanoso)以及绿原酸(CGA:380.22 ± 22.05 mg/100 g)(基因型为 Copena V1)。在分析不同果实色素的基因型时,我们还观察到:4,5-雌二醇二氧酶可存在于所有基因型中(与色素无关);5,3-葡糖基转移酶(GTs)可在橙色至紫色果实基因型的色素合成中被诱导。GT 的引物扩增片段测序结果显示与其他物种的尿苷二磷酸(UDP)-葡萄糖高度相似。这使我们可以推断,在不久的将来,在可控条件下有可能获得具有很高治疗价值的产品。
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Interaction of Culture Medium and Artificial Light Type on Pigmentation of Micro-Propagated Opuntia Plants
Opuntia is recognized economically as a significant crop for its nutritional, functional, and therapeutic properties and its potential in the pharmaceutical, cosmetic, and bioenergy industries. Opuntia is conventionally propagated by seed or vegetative propagation via rooted scions or grafting. However, multiplication procedures are insufficient for extensive spreading. One of the alternative techniques is in vitro. For this reason, the objective was to evaluate the growth of different components combined with light conditions to induce callus, embryogenesis, and the possible synthesis of a component in the genus Opuntia. The existence of genes involved in pigment synthesis in genotypes of different fruit colors was complementarily analyzed. In this study, we used different combinations of fructose (2 g/L) with prickly pear juice (2 mL/L), mannitol (4 g/L), silver nitrate (AgNO3: 1 mg/L), 2,4-dichlorophenoxyacetic acid (2,4-D: 2 mg/L), and indole-3-acetic acid (IAA: 1 g/L), with white, blue, and red LEDs (light-emitting diodes) and laser beams. These explants yielded sufficient contents of simple phenols, gallic acid equivalents (GAE: 2283.30 ± 6.29 mg/100 g), and coumaric (2155.0 ± 35.0 mg/100 g) and ferulic (2176 ± 27.9 mg/100 g) acids for the genotype Tapón aguanoso, and chlorogenic acid (CGA: 380.22 ± 22.05 mg/100 g) for the Copena V1 genotype. Upon analyzing the genotypes of different fruit pigmentations, we also observed the following: enzyme 4,5 estradiol dioxygenases can be present in all genotypes (regardless of pigmentation); enzyme 5,3 glucosyltransferases (GTs) could be induced in pigment synthesis in the genotypes of orange to purple fruits. Sequencing primer-amplified fragments for GT showed high similarity to uridine diphospho (UDP)-glucose from other species. This allows us to infer that it is possible to obtain products of high therapeutic value in the near future under controlled conditions.
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来源期刊
Horticulturae
Horticulturae HORTICULTURE-
CiteScore
3.50
自引率
19.40%
发文量
998
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