敲除 BAX 和 BAK1 基因以及过表达 BCL2 和 BECN1 基因可提高 CHO-S 细胞培养物的寿命和最大密度

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2023-12-14 DOI:10.1134/S0003683823080057

S. V. Kovnir, L. K. Dayanova, E. A. Gaiamova, L. N. Dybovsky, I. I. Vorobiev, N. A. Orlova

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引用次数: 0

摘要

经过两次基因组改造，CHO 细胞系 4BGD 诞生了，它同源敲除了 BAK1、BAX、DHFR 和 GLUL 基因，并过表达了 BCL2 和 BECN1 基因。这种细胞系能够在不改变培养基的情况下长期生长，而且在相同的培养条件下，其完整细胞密度比亲本细胞系增加了五倍。与敲除了 BAK1、BAX、DHFR 和 GLUL 基因的细胞系相比，BCL2、BECN1 基因对的过表达导致细胞密度在培养的第 6-10 天显著增加，而整体存活细胞密度增加了 34%。

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Knockout of BAX and BAK1 Genes and Overexpression of BCL2, BECN1 Genes Increase Lifespan and the Maximum Density of a CHO-S Cell Culture

Two genome editions have resulted in the CHO cell line 4BGD with homozygous knockouts of the BAK1, BAX, DHFR and GLUL genes, and overexpression of the BCL2 and BECN1 genes. This line is capable of long-term growth without changing the culture medium and exhibits a fivefold increase in the integral cell density compared to the parental cell line under the same cultivation regime. Overexpression of the BCL2, BECN1 gene pair led to a significant increase in cell density on days 6‒10 of cultivation compared to the cell line with a knockout of the BAK1, BAX, DHFR and GLUL genes, while the integral viable cell density increases by 34%.

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.